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The map label for this gene is groEL

Identifier: 49476034

GI number: 49476034

Start: 1547627

End: 1549270

Strand: Reverse

Name: groEL

Synonym: BH13530

Alternate gene names: 49476034

Gene position: 1549270-1547627 (Counterclockwise)

Preceding gene: 49476035

Following gene: 49476033

Centisome position: 80.23

GC content: 44.16

Gene sequence:

>1644_bases
ATGGCTGCTAAAGAAGTCAAGTTTGGCCGTGAAGCGCGTGAGCGTTTGTTGCGCGGCGTTGATATCCTTGCTAACGCTGT
TAAAGTAACACTCGGCCCTAAAGGGCGCAATGTGGTGATCGATAAATCATTTGGTGCGCCTCGCATTACAAAAGATGGTG
TATCCGTTGCAAAAGAAATCGAACTGGAAGATAAGTTCGAAAATATGGGTGCGCAAATGTTGCGTGAAGTTGCTTCTAAA
ACCAATGACATTGCTGGTGATGGAACAACAACGGCAACTGTTTTAGGACAGGCTATTGTCCAAGAAGGTGTAAAAGCCGT
TGCTGCAGGCATGAACCCAATGGATCTCAAACGTGGTATCGATGCTGCTGTTGATGAAGTGGTGGCAAATCTTTTCAAAA
AAGCGAAAAAAATCCAAACTTCAGCAGAAATTGCACAAGTGGGAACAATTTCCGCTAATGGTGCTGCTGAAATCGGCAAA
ATGATCGCTGATGCCATGGAAAAAGTTGGCAATGAAGGTGTCATTACCGTTGAAGAAGCAAAAACTGCTGAAACGGAATT
AGAAGTCGTTGAAGGAATGCAGTTTGATCGTGGATATCTTTCCCCTTACTTTGTCACCAATGCTGAGAAAATGGTGGCTG
ATCTTGATGATCCTTACATTCTTATTCACGAAAAGAAACTCTCTAATCTCCAATCCTTGCTTCCAGTACTTGAAGCTGTT
GTTCAGTCTGGTAAACCACTTCTCATTATCGCTGAAGATGTGGAAGGTGAAGCTTTGGCAACGCTCGTTGTCAACAAACT
GCGTGGTGGTTTGAAAATTGCTGCTGTGAAAGCTCCAGGATTTGGTGATCGCCGTAAAGCCATGTTAGAAGATATTGCAA
TCTTAACATCCGGACAGGTTATTTCTGAAGATGTTGGCATTAAATTGGAAAATGTCACTTTGGATATGCTTGGTCGTGCA
AAGAAAGTCAATATTTCTAAAGAAAATACCACGATTATTGATGGTGCTGGACAAAAGAGCGAAATTAATGCGCGCGTCAA
CCAAATCAAGGTACAGATCGAAGAAACAACTTCTGACTATGACCGTGAAAAATTGCAAGAAAGACTTGCTAAACTCGCTG
GAGGTGTTGCTGTTATCCGTGTTGGTGGAGCAACAGAAGTTGAAGTGAAAGAAAAGAAAGATCGTGTTGATGATGCCTTG
AACGCAACACGTGCAGCTGTGGAAGAAGGTATTGTTGCCGGTGGTGGAACCGCATTGTTGCGTGCAGCAAATGCCCTGAC
TGTTAAAGGAAGCAATCCTGATCAGGAAGCTGGTATCAATATTGTTCGTCGTGCACTCCAAGCGCCAGCACGCCAAATTG
CTACCAATGCCGGTGAAGAAGCAGCGATTATCGTTGGCAAAGTGCTTGAAAACAATGCAGATACATTTGGTTACAACACC
GCAACCGGTGAATTTGGTGATTTGATTGCTTTGGGAATCGTTGATCCTGTGAAGGTTGTGCGCTCTGCTCTTCAGAATGC
TGCCTCAATTGCTAGCCTTCTCATTACAACAGAAGCAATGGTTGCTGAAGTTCCAAAGAAAGACACACCAGTGCCTCCAA
TGCCTGGTGGCGGAATGGGTGGAATGGGCGGAATGGATTTCTAA

Upstream 100 bases:

>100_bases
ATCATGAAAGAGTCTGACATTATGGGGATTTTGGGTTAATCCAACCTCTCAATTTTTTTCATTTTCTACTATTTAGGACA
TAACTCCAAGGAGAAATTAA

Downstream 100 bases:

>100_bases
GCCCTTGATAAAGATAAAAGAAAACGGGCCTCTCTGGCCCGTTTTTTTGTAGGTTTTTCCTGTCTCTTATCAAATTTCTC
CATTTTTTACGATTTTCTGG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Heat shock protein 60; Protein Cpn60

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKEVKFGREARERLLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMLREVASK
TNDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKRGIDAAVDEVVANLFKKAKKIQTSAEIAQVGTISANGAAEIGK
MIADAMEKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNAEKMVADLDDPYILIHEKKLSNLQSLLPVLEAV
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSGQVISEDVGIKLENVTLDMLGRA
KKVNISKENTTIIDGAGQKSEINARVNQIKVQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVAGGGTALLRAANALTVKGSNPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLENNADTFGYNT
ATGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAEVPKKDTPVPPMPGGGMGGMGGMDF

Sequences:

>Translated_547_residues
MAAKEVKFGREARERLLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMLREVASK
TNDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKRGIDAAVDEVVANLFKKAKKIQTSAEIAQVGTISANGAAEIGK
MIADAMEKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNAEKMVADLDDPYILIHEKKLSNLQSLLPVLEAV
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSGQVISEDVGIKLENVTLDMLGRA
KKVNISKENTTIIDGAGQKSEINARVNQIKVQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDAL
NATRAAVEEGIVAGGGTALLRAANALTVKGSNPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLENNADTFGYNT
ATGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAEVPKKDTPVPPMPGGGMGGMGGMDF
>Mature_546_residues
AAKEVKFGREARERLLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMLREVASKT
NDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKRGIDAAVDEVVANLFKKAKKIQTSAEIAQVGTISANGAAEIGKM
IADAMEKVGNEGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNAEKMVADLDDPYILIHEKKLSNLQSLLPVLEAVV
QSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSGQVISEDVGIKLENVTLDMLGRAK
KVNISKENTTIIDGAGQKSEINARVNQIKVQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALN
ATRAAVEEGIVAGGGTALLRAANALTVKGSNPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLENNADTFGYNTA
TGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAEVPKKDTPVPPMPGGGMGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=53.219696969697, Blast_Score=559, Evalue=1e-159,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=53.219696969697, Blast_Score=559, Evalue=1e-159,
Organism=Homo sapiens, GI5453607, Length=552, Percent_Identity=22.2826086956522, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790586, Length=529, Percent_Identity=66.7296786389414, Blast_Score=691, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=52.8409090909091, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=53.8095238095238, Blast_Score=222, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17564182, Length=558, Percent_Identity=22.2222222222222, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=57.9847908745247, Blast_Score=600, Evalue=1e-172,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=56.25, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=56.25, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52.5714285714286, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52.5714285714286, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52.5714285714286, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI17864606, Length=549, Percent_Identity=43.7158469945355, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24584129, Length=537, Percent_Identity=36.8715083798883, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI19921262, Length=537, Percent_Identity=36.8715083798883, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_BARHE (O33963)

Other databases:

- EMBL:   U78514
- EMBL:   AJ749669
- EMBL:   BX897699
- EMBL:   AF014829
- EMBL:   U96734
- RefSeq:   YP_034075.1
- ProteinModelPortal:   O33963
- SMR:   O33963
- GeneID:   2865623
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH13530
- NMPDR:   fig|283166.1.peg.1203
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- PhylomeDB:   O33963
- ProtClustDB:   PRK00013
- BioCyc:   BHEN283166:BH13530-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57626; Mature: 57495

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEVKFGREARERLLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMLREVASKTNDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DAAVDEVVANLFKKAKKIQTSAEIAQVGTISANGAAEIGKMIADAMEKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNAEKMVADLDDPYILIHEKKLSNLQSLLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHH
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
ISEDVGIKLENVTLDMLGRAKKVNISKENTTIIDGAGQKSEINARVNQIKVQIEETTSDY
EHHCCCEEEEEEEHHHHCCCCEEECCCCCCEEEECCCCCHHHHHEEEEEEEEEECCCCCC
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVAGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAANALTVKGSNPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLENNADTFGYNT
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCC
ATGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAEVPKKDTPVPPMPGGGMG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC
>Mature Secondary Structure 
AAKEVKFGREARERLLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
ELEDKFENMGAQMLREVASKTNDIAGDGTTTATVLGQAIVQEGVKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DAAVDEVVANLFKKAKKIQTSAEIAQVGTISANGAAEIGKMIADAMEKVGNEGVITVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNAEKMVADLDDPYILIHEKKLSNLQSLLPVLEAV
CCHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHH
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTSGQV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCE
ISEDVGIKLENVTLDMLGRAKKVNISKENTTIIDGAGQKSEINARVNQIKVQIEETTSDY
EHHCCCEEEEEEEHHHHCCCCEEECCCCCCEEEECCCCCHHHHHEEEEEEEEEECCCCCC
DREKLQERLAKLAGGVAVIRVGGATEVEVKEKKDRVDDALNATRAAVEEGIVAGGGTALL
HHHHHHHHHHHHHCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAANALTVKGSNPDQEAGINIVRRALQAPARQIATNAGEEAAIIVGKVLENNADTFGYNT
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCC
ATGEFGDLIALGIVDPVKVVRSALQNAASIASLLITTEAMVAEVPKKDTPVPPMPGGGMG
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274034; 9230387