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The map label for this gene is mraW

Identifier: 49475863

GI number: 49475863

Start: 1249566

End: 1250606

Strand: Reverse

Name: mraW

Synonym: BH11320

Alternate gene names: 49475863

Gene position: 1250606-1249566 (Counterclockwise)

Preceding gene: 49475864

Following gene: 49475862

Centisome position: 64.76

GC content: 42.75

Gene sequence:

>1041_bases
ATGGCTATTGATTTAAAAAAGCATAAATCAAGACGGATTATTTTGACAAAACAGGGTAAGAGAACTGAGCGCCATATTCC
AGTGTTGTTACAGCCAGTTTTAGCTGGGCTTATACCATTGGTTGGGGCAAAAGTGATTGATGGCACCTTTGGTGCTGGTG
GTTATACACGTGCTTTGTTAAATGCGGGTGCACAGGTTATTGCTCTTGATCGTGATCCTCACGCTATTCGTGAAGGACAA
TCGCTTGTTGATGAATTTTTTCCACGACTTCGTTTGGTGCAAGGGAATTTTTCACAGTTGGATTGTGTGGTTGAAGAGAA
GGTAGATGCCGTTATTTTGGATATTGGTGTCTCTTCAATGCAGCTTGATGAAGCTGAAAGAGGATTTTCTTTTCAAAAAG
ATGGTCCATTAGATATGCGAATGGCTCAGACTGGTTTTACTGCTGCTGATGTTGTGAATCGTTTAAAAAGTGATGAGTTA
GCGCGAATCTTTAAAATATTAGGAGAAGAACGCTATGCAAGGCGAATTGCACGGATGATTGAAAAGCGCCGCTGTGTTCA
ACCGTTTTTGCGTACAGGTGATCTTGCTCATGCCATTGAGGTGTTGGTGGGGCGTAAAAGCGGAGAGCGCATTCATCCTG
CAACGCGTGTATTTCAGGCTCTTCGCATTTATGTGAACGATGAAATTGGTGAACTTGCGCGTGGCTTATTTGCTGCTGAA
CGCGTTTTGAAACCAGGAGGTCGTTTGGGCGTCGTGAGTTTTCATTCTCTTGAAGATCGTATGGTTAAAAGATTTTTTTC
TGCTCGTTCAGGAGAAAGTGTGAGATCTCGCTATCTTCCTGAAATAGAGATGGCTCCAGCAACATTTTTTCCTTTATTTA
AAGGGGGGATAACTGCAAATAAAGAGGAGTTGGAGCGAAATCCTCGTTCACGTTCTGCGAGGTTGCGTATTGGTGTACGT
ACTGAGGCTGAGTGTCTTTTTGCAGATATGAAATTGTTTGGGTTAGCAAAGATTGCCAGTTTTGAAGGTGGCAAGAAATG
A

Upstream 100 bases:

>100_bases
TGTGTTAGGATGTGTATCCGGTTAACTTTAGCTTCATTTCCTTTCATTATTATACAGAGGAAAGTTGAAGTTTTAGTGAT
CCTGACATGAGTTGCCATTG

Downstream 100 bases:

>100_bases
CAGTTTTTCGTACATTTGATATGATTTTAGTCATGATTATGATTTGTATGGCAGGTCTTACTTATAAAGTCAAATATGAT
GTCCAAAAACGAATGAGTGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALLNAGAQVIALDRDPHAIREGQ
SLVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSMQLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDEL
ARIFKILGEERYARRIARMIEKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAE
RVLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITANKEELERNPRSRSARLRIGVR
TEAECLFADMKLFGLAKIASFEGGKK

Sequences:

>Translated_346_residues
MAIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALLNAGAQVIALDRDPHAIREGQ
SLVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSMQLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDEL
ARIFKILGEERYARRIARMIEKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAE
RVLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITANKEELERNPRSRSARLRIGVR
TEAECLFADMKLFGLAKIASFEGGKK
>Mature_345_residues
AIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALLNAGAQVIALDRDPHAIREGQS
LVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSMQLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDELA
RIFKILGEERYARRIARMIEKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAER
VLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITANKEELERNPRSRSARLRIGVRT
EAECLFADMKLFGLAKIASFEGGKK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=39.4029850746269, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=41.7112299465241, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=43.0817610062893, Blast_Score=219, Evalue=3e-58,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=39.039039039039, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=39.039039039039, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BARHE (Q6G2P7)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033904.1
- ProteinModelPortal:   Q6G2P7
- SMR:   Q6G2P7
- GeneID:   2865755
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH11320
- NMPDR:   fig|283166.1.peg.1032
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q6G2P7
- ProtClustDB:   PRK00050
- BioCyc:   BHEN283166:BH11320-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 38657; Mature: 38525

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: NA

Important sites: BINDING 70-70 BINDING 97-97 BINDING 114-114 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALL
CCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
NAGAQVIALDRDPHAIREGQSLVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSM
HCCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCEEEEECCCCHH
QLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDELARIFKILGEERYARRIARMI
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHH
EKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAE
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RVLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITAN
HHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCC
KEELERNPRSRSARLRIGVRTEAECLFADMKLFGLAKIASFEGGKK
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AIDLKKHKSRRIILTKQGKRTERHIPVLLQPVLAGLIPLVGAKVIDGTFGAGGYTRALL
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
NAGAQVIALDRDPHAIREGQSLVDEFFPRLRLVQGNFSQLDCVVEEKVDAVILDIGVSSM
HCCCEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCEEEEECCCCHH
QLDEAERGFSFQKDGPLDMRMAQTGFTAADVVNRLKSDELARIFKILGEERYARRIARMI
HHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHHHH
EKRRCVQPFLRTGDLAHAIEVLVGRKSGERIHPATRVFQALRIYVNDEIGELARGLFAAE
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RVLKPGGRLGVVSFHSLEDRMVKRFFSARSGESVRSRYLPEIEMAPATFFPLFKGGITAN
HHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCC
KEELERNPRSRSARLRIGVRTEAECLFADMKLFGLAKIASFEGGKK
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA