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The map label for this gene is murD

Identifier: 49475857

GI number: 49475857

Start: 1242034

End: 1243434

Strand: Reverse

Name: murD

Synonym: BH11260

Alternate gene names: 49475857

Gene position: 1243434-1242034 (Counterclockwise)

Preceding gene: 49475858

Following gene: 49475856

Centisome position: 64.39

GC content: 39.69

Gene sequence:

>1401_bases
TTGATTTCTATTGCGTTTTATAAGGGGAAAAAAGTTGCTCTGTTTGGATTAGGAAAATCCGGATTAGCTACTGCACAAGC
ATTGATAAGTGGTGGTGCAGACGTAGTCGCCTGGGATGATAATCCTTCAGGTGTACAAGCGGCTCATAGAGAAAATATTC
CAGCGAGAAATCTGCAATATGAAAATTGGTCAGAATTTGTTGCATTGATTCTTGCTCCGGGCGTTCCTTTAACTTATCCT
CAGCCGCATTGGGTTGTTGATAAAGCACGTCAAGCAAATATAGAAATTATCGGTGATATTGAGTTATTCGTGCGTGCACG
TAACCATTTTTTACAACAGTATGGTTTTTGTGATGAGGATGTTCCTTTTATCGCTATTACGGGCACGAATGGGAAATCAA
CAACAACAGCTTTGCTTGCTCACTTGTTGGAACAAATGGGTTATGATGTGCAGATGGGAGGAAATATTGGAACGGCAATA
TTAACGCTTAAACCATTTGTTAAAAAACGTATTTATGTGATTGAATGTTCGTCGTTTCAAATTGATCTCGCTCCTTCTCT
TCAGCCAACTATTGGGCTTTTATTGAATCTAACGCCTGATCATATTGATCGCCATGGGAGTTTTGCTCATTATGTGAACG
CTAAAAAGAATTTAGTGACTGGGGCTTCTCAAGCTTTTATTTCAGTTGATGATGCCGCTTGTCAAGTTTTGTATCGACAA
TTGCTTCATGAAGGTCATCACGTTGAGGCAGTTTCTAAGGAACATTTTGTTGAGAATGGTTTTTACGCTGATGGAACTCA
GCTTTTTTCTGTTTGCCAAGGTCGGCGTCATATGCTTGCAGATCTCGCGTCTATGGCTGCATTGCGCGGAAGTCATAATG
CGCAAAATGCTCTTATGGCATTAGCAACATTGCAAGCTTTAAAAATAACCGATCCCCATATGAACAAACATTTAGCAAGT
TATCAAGGGTTAGCTCATCGTATGCAGCAGGTGCGTAAAATGGGATCAGTTTTGTTTATCAATGATAGCAAGGCTACCAA
TGCAGAGGCATCAGCTCCTGCACTTGCTGCTTTTAATAATATTTTTTGGATTGTTGGAGGACAGGCGAAAGAAGCAGGAA
TTGTTTCTCTTAGAGGATTTTTTCATAAAATTCGCAAAGCTTATTTGATTGGAGCTGCGGCAGAAGAATTTGCTGGTGTT
ATTGGATCTTCTTTTCCTTTTTCGATGAGTTTAACTTTGGAAAATGCGGTGCATGAAGCCGCTGTTGATGCAATGCGTTG
TAAAGCGAAAGAAGTTGTGGTTCTCTTTTCACCTGCTTGTGCAAGTTATGATCAATTTAAAAACTATGAAGTGCGTGGCG
AAGCATTTATTTCTTTTGTTATGCAGTTAAAAGAAACGTAA

Upstream 100 bases:

>100_bases
AAAAAAGGCTGGACTGAAAGTCAAGTCGTGATACGCTTTTGGATTATTTCGATTGTTCTTGCTCTCATTGGTCTTTCAAC
ACTTAAATTGCGGTGAAATT

Downstream 100 bases:

>100_bases
ATTATAAAAAATGTTTTTACGTTAATATTCCGGAATCTTTTCTCATGTAATTCAGTTTTTGAAGTGTTTGCTAAAAAAGT
GGGGAACCATGTCACTTTGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MISIAFYKGKKVALFGLGKSGLATAQALISGGADVVAWDDNPSGVQAAHRENIPARNLQYENWSEFVALILAPGVPLTYP
QPHWVVDKARQANIEIIGDIELFVRARNHFLQQYGFCDEDVPFIAITGTNGKSTTTALLAHLLEQMGYDVQMGGNIGTAI
LTLKPFVKKRIYVIECSSFQIDLAPSLQPTIGLLLNLTPDHIDRHGSFAHYVNAKKNLVTGASQAFISVDDAACQVLYRQ
LLHEGHHVEAVSKEHFVENGFYADGTQLFSVCQGRRHMLADLASMAALRGSHNAQNALMALATLQALKITDPHMNKHLAS
YQGLAHRMQQVRKMGSVLFINDSKATNAEASAPALAAFNNIFWIVGGQAKEAGIVSLRGFFHKIRKAYLIGAAAEEFAGV
IGSSFPFSMSLTLENAVHEAAVDAMRCKAKEVVVLFSPACASYDQFKNYEVRGEAFISFVMQLKET

Sequences:

>Translated_466_residues
MISIAFYKGKKVALFGLGKSGLATAQALISGGADVVAWDDNPSGVQAAHRENIPARNLQYENWSEFVALILAPGVPLTYP
QPHWVVDKARQANIEIIGDIELFVRARNHFLQQYGFCDEDVPFIAITGTNGKSTTTALLAHLLEQMGYDVQMGGNIGTAI
LTLKPFVKKRIYVIECSSFQIDLAPSLQPTIGLLLNLTPDHIDRHGSFAHYVNAKKNLVTGASQAFISVDDAACQVLYRQ
LLHEGHHVEAVSKEHFVENGFYADGTQLFSVCQGRRHMLADLASMAALRGSHNAQNALMALATLQALKITDPHMNKHLAS
YQGLAHRMQQVRKMGSVLFINDSKATNAEASAPALAAFNNIFWIVGGQAKEAGIVSLRGFFHKIRKAYLIGAAAEEFAGV
IGSSFPFSMSLTLENAVHEAAVDAMRCKAKEVVVLFSPACASYDQFKNYEVRGEAFISFVMQLKET
>Mature_466_residues
MISIAFYKGKKVALFGLGKSGLATAQALISGGADVVAWDDNPSGVQAAHRENIPARNLQYENWSEFVALILAPGVPLTYP
QPHWVVDKARQANIEIIGDIELFVRARNHFLQQYGFCDEDVPFIAITGTNGKSTTTALLAHLLEQMGYDVQMGGNIGTAI
LTLKPFVKKRIYVIECSSFQIDLAPSLQPTIGLLLNLTPDHIDRHGSFAHYVNAKKNLVTGASQAFISVDDAACQVLYRQ
LLHEGHHVEAVSKEHFVENGFYADGTQLFSVCQGRRHMLADLASMAALRGSHNAQNALMALATLQALKITDPHMNKHLAS
YQGLAHRMQQVRKMGSVLFINDSKATNAEASAPALAAFNNIFWIVGGQAKEAGIVSLRGFFHKIRKAYLIGAAAEEFAGV
IGSSFPFSMSLTLENAVHEAAVDAMRCKAKEVVVLFSPACASYDQFKNYEVRGEAFISFVMQLKET

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=475, Percent_Identity=31.5789473684211, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_BARHE (Q6G2Q3)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033898.1
- ProteinModelPortal:   Q6G2Q3
- SMR:   Q6G2Q3
- GeneID:   2865364
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH11260
- NMPDR:   fig|283166.1.peg.1026
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q6G2Q3
- ProtClustDB:   PRK01390
- BioCyc:   BHEN283166:BH11260-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50884; Mature: 50884

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIAFYKGKKVALFGLGKSGLATAQALISGGADVVAWDDNPSGVQAAHRENIPARNLQY
CEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCC
ENWSEFVALILAPGVPLTYPQPHWVVDKARQANIEIIGDIELFVRARNHFLQQYGFCDED
CCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
VPFIAITGTNGKSTTTALLAHLLEQMGYDVQMGGNIGTAILTLKPFVKKRIYVIECSSFQ
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECHHHHCEEEEEEECCEE
IDLAPSLQPTIGLLLNLTPDHIDRHGSFAHYVNAKKNLVTGASQAFISVDDAACQVLYRQ
EEECCCCCCCCCEEEECCHHHHHCCCCCHHHHCCCHHHCCCCCCEEEEECHHHHHHHHHH
LLHEGHHVEAVSKEHFVENGFYADGTQLFSVCQGRRHMLADLASMAALRGSHNAQNALMA
HHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LATLQALKITDPHMNKHLASYQGLAHRMQQVRKMGSVLFINDSKATNAEASAPALAAFNN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCC
IFWIVGGQAKEAGIVSLRGFFHKIRKAYLIGAAAEEFAGVIGSSFPFSMSLTLENAVHEA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
AVDAMRCKAKEVVVLFSPACASYDQFKNYEVRGEAFISFVMQLKET
HHHHHHCCCCEEEEEECCCCCCHHHHCCCEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MISIAFYKGKKVALFGLGKSGLATAQALISGGADVVAWDDNPSGVQAAHRENIPARNLQY
CEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCC
ENWSEFVALILAPGVPLTYPQPHWVVDKARQANIEIIGDIELFVRARNHFLQQYGFCDED
CCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCC
VPFIAITGTNGKSTTTALLAHLLEQMGYDVQMGGNIGTAILTLKPFVKKRIYVIECSSFQ
CCEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECHHHHCEEEEEEECCEE
IDLAPSLQPTIGLLLNLTPDHIDRHGSFAHYVNAKKNLVTGASQAFISVDDAACQVLYRQ
EEECCCCCCCCCEEEECCHHHHHCCCCCHHHHCCCHHHCCCCCCEEEEECHHHHHHHHHH
LLHEGHHVEAVSKEHFVENGFYADGTQLFSVCQGRRHMLADLASMAALRGSHNAQNALMA
HHHCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LATLQALKITDPHMNKHLASYQGLAHRMQQVRKMGSVLFINDSKATNAEASAPALAAFNN
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCC
IFWIVGGQAKEAGIVSLRGFFHKIRKAYLIGAAAEEFAGVIGSSFPFSMSLTLENAVHEA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
AVDAMRCKAKEVVVLFSPACASYDQFKNYEVRGEAFISFVMQLKET
HHHHHHCCCCEEEEEECCCCCCHHHHCCCEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA