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The map label for this gene is ddl

Identifier: 49475852

GI number: 49475852

Start: 1236106

End: 1237026

Strand: Reverse

Name: ddl

Synonym: BH11210

Alternate gene names: 49475852

Gene position: 1237026-1236106 (Counterclockwise)

Preceding gene: 49475853

Following gene: 49475851

Centisome position: 64.06

GC content: 42.35

Gene sequence:

>921_bases
ATGAAAGATGAGCATATAGCTGTGCTGATGGGGGGATTCTCTTCTGAACGGTCTGTAAGTTTGTCTTCAGGTACTGCTTG
TGCTGATGTTCTTGAAGCGCAGGGGTATCGTGTGAGTCGTGTGGATGTTGATGGTCATATCGCTTCTGTTCTTGAACAGT
TGCAGCCTGATATTGCTTTTAATGCGTTACATGGTCCATTTGGTGAAGATGGTCGTATTCAGGGTGTTCTTGAATATTTA
AAAATTCCTTATACGCATTCTGGCGTGATGGCATCAGCACTGGCAATGGATAAAGGGCGTGCAAAAATTGTTGTTGCAAG
CGTTGGGGTTTCTATTGCTCCTTCTTGTGTCATGAGTCGCTTTGCTGTTGGGAAAGAGCATCCTATGGAGCCTCCTTACG
TGATTAAGCCTGTCTGTGAAGGGTCAAGTTTGGGTGTGATTATTGTAAAAGAAAATGAATCTGTGCCATCGCTTAATGTT
GTGGGATCTGAATGGGTTTATGCAGATACAGTAATTGTTGAAAAATATATTCCTGGTCGTGAGCTTACTTGTGCTGTTAT
GGGAAACAAAGCTCTGGATGTTTGTGAAATTCTTCCTGAAAAGCACTTTCAATTCTACGATTATGACTCAAAATATAAAT
CTGGTGGCTCTCTTCATGTTTGTCCTGCACAACTTTCATCAAATATTTACCAAAATGTGCAAAGAATGTCTTTAGCAGCA
CATCAGGCGATAGGTTGTCGTGGTGTTAGCCGTTCTGATTTTCGTTTTGATGAGGAAACAGGAGAGTTGGTTTGGCTTGA
AATTAATACACAACCCGGTATGACGCCAACATCTCTTTTCCCTGATATTGCAAAAGCGAGTGGTCGTACTTACGGCGATA
TTGTTCAGTGGATGGTGGAGGACGCGTCATGTATGCGTTGA

Upstream 100 bases:

>100_bases
AGGTTATTGATTCGCAAGGGTAAATCTTGGTCTTTCCTGTATATGGTGTTAGTGATTTCTGTGTTGTCTATATATAGTAT
ACGAAGGAGAATAAGTTGTT

Downstream 100 bases:

>100_bases
ATGTTGATAAAACAAATATCCCCATGGTGGTACTTTCAGTGCCAGTTTTGCCACGTCTTTATCGTCGCTTTCTCCGGTTT
ATGTTTGAGTTTGTATTTGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKDEHIAVLMGGFSSERSVSLSSGTACADVLEAQGYRVSRVDVDGHIASVLEQLQPDIAFNALHGPFGEDGRIQGVLEYL
KIPYTHSGVMASALAMDKGRAKIVVASVGVSIAPSCVMSRFAVGKEHPMEPPYVIKPVCEGSSLGVIIVKENESVPSLNV
VGSEWVYADTVIVEKYIPGRELTCAVMGNKALDVCEILPEKHFQFYDYDSKYKSGGSLHVCPAQLSSNIYQNVQRMSLAA
HQAIGCRGVSRSDFRFDEETGELVWLEINTQPGMTPTSLFPDIAKASGRTYGDIVQWMVEDASCMR

Sequences:

>Translated_306_residues
MKDEHIAVLMGGFSSERSVSLSSGTACADVLEAQGYRVSRVDVDGHIASVLEQLQPDIAFNALHGPFGEDGRIQGVLEYL
KIPYTHSGVMASALAMDKGRAKIVVASVGVSIAPSCVMSRFAVGKEHPMEPPYVIKPVCEGSSLGVIIVKENESVPSLNV
VGSEWVYADTVIVEKYIPGRELTCAVMGNKALDVCEILPEKHFQFYDYDSKYKSGGSLHVCPAQLSSNIYQNVQRMSLAA
HQAIGCRGVSRSDFRFDEETGELVWLEINTQPGMTPTSLFPDIAKASGRTYGDIVQWMVEDASCMR
>Mature_306_residues
MKDEHIAVLMGGFSSERSVSLSSGTACADVLEAQGYRVSRVDVDGHIASVLEQLQPDIAFNALHGPFGEDGRIQGVLEYL
KIPYTHSGVMASALAMDKGRAKIVVASVGVSIAPSCVMSRFAVGKEHPMEPPYVIKPVCEGSSLGVIIVKENESVPSLNV
VGSEWVYADTVIVEKYIPGRELTCAVMGNKALDVCEILPEKHFQFYDYDSKYKSGGSLHVCPAQLSSNIYQNVQRMSLAA
HQAIGCRGVSRSDFRFDEETGELVWLEINTQPGMTPTSLFPDIAKASGRTYGDIVQWMVEDASCMR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=37.2168284789644, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1786579, Length=354, Percent_Identity=31.3559322033898, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BARHE (Q6G2Q8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033893.1
- ProteinModelPortal:   Q6G2Q8
- SMR:   Q6G2Q8
- GeneID:   2864509
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH11210
- NMPDR:   fig|283166.1.peg.1021
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q6G2Q8
- ProtClustDB:   PRK01372
- BioCyc:   BHEN283166:BH11210-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33259; Mature: 33259

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEHIAVLMGGFSSERSVSLSSGTACADVLEAQGYRVSRVDVDGHIASVLEQLQPDIAF
CCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEE
NALHGPFGEDGRIQGVLEYLKIPYTHSGVMASALAMDKGRAKIVVASVGVSIAPSCVMSR
ECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FAVGKEHPMEPPYVIKPVCEGSSLGVIIVKENESVPSLNVVGSEWVYADTVIVEKYIPGR
HHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCEEEEEEEEEEECCCCC
ELTCAVMGNKALDVCEILPEKHFQFYDYDSKYKSGGSLHVCPAQLSSNIYQNVQRMSLAA
CEEEEEECCCHHHHHHHCHHHCCEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
HQAIGCRGVSRSDFRFDEETGELVWLEINTQPGMTPTSLFPDIAKASGRTYGDIVQWMVE
HHHHCCCCCCCCCCEECCCCCCEEEEEECCCCCCCHHHHCHHHHHHCCCCHHHHHHHHHH
DASCMR
HHHHCC
>Mature Secondary Structure
MKDEHIAVLMGGFSSERSVSLSSGTACADVLEAQGYRVSRVDVDGHIASVLEQLQPDIAF
CCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCCCEE
NALHGPFGEDGRIQGVLEYLKIPYTHSGVMASALAMDKGRAKIVVASVGVSIAPSCVMSR
ECCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
FAVGKEHPMEPPYVIKPVCEGSSLGVIIVKENESVPSLNVVGSEWVYADTVIVEKYIPGR
HHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCEEEEEEEEEEECCCCC
ELTCAVMGNKALDVCEILPEKHFQFYDYDSKYKSGGSLHVCPAQLSSNIYQNVQRMSLAA
CEEEEEECCCHHHHHHHCHHHCCEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
HQAIGCRGVSRSDFRFDEETGELVWLEINTQPGMTPTSLFPDIAKASGRTYGDIVQWMVE
HHHHCCCCCCCCCCEECCCCCCEEEEEECCCCCCCHHHHCHHHHHHCCCCHHHHHHHHHH
DASCMR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA