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The map label for this gene is rpoA

Identifier: 49475770

GI number: 49475770

Start: 1142799

End: 1143812

Strand: Reverse

Name: rpoA

Synonym: BH10270

Alternate gene names: 49475770

Gene position: 1143812-1142799 (Counterclockwise)

Preceding gene: 49475771

Following gene: 49475769

Centisome position: 59.23

GC content: 39.05

Gene sequence:

>1014_bases
ATGATCCAGAAAAACTGGCAGGAACTCATTAAACCCAATAAGGTTGAGTTTTCTAAACATGATAATCCGAATATTTCAAG
CGTGATTGTGGAGCCTCTTGAGCGGGGTTTTGGCTTAACATTAGGCAATGCGCTTCGTCGCGTATTATTGTCATCACTTC
GTGGTGCTGCAATTACTGCTGTACAGATTGATGGTGTCTTACATGAATTTTCATCAATTCCGGGTGTTCGTGAGGATGTT
ACAGATATTATTTTGAATATCAAAGAAATTGCACTTCGTATGCGAGAAGAGGGGCCTAAACGTATTGTTGTTTGTAAAGA
GGGCCCTGGTGTTCTGAGAGCTGGGGATATCAGTACCGTTGGAGATATGGAAATTTTGAATCCAGATCATGTTATCTGTA
CTCTTGATGAGGATGCTGAGATTCGTATGGAATTTATTGTTAATACAGGGAAAGGTTATGTGCCCTCTGATCGCAATTGT
ATTGATGATGCGCGAATAGGTCTTATTCCAGTTGATAGTCTTTATTCACCAATTCGTAAAGTGTCTTATAAGGTAGAGAA
TACCCGTGAAGGTCAAGTTCTGGATTATGATAAATTAACGCTTACAGTTGAAACGAATGGTGCTGTTAATGGAGAGGATG
CTCTTGCTTTTGCAGCACGTATCCTTCAGGATCAATTAGCGTTGTTTATCAATTTTAAGGAGCCAGAGAAGCCAACTGTT
GAAGAGTCTAATTCAGAGCTTGCTTTTAATCCCGCGCTTCTCAAGAAAGTGGATGAATTAGAGCTTTCAGTTCGTTCGGC
AAATTGTCTTAAGAATGATAATATTGTTTATATTGGTGATCTTATCCAAAAAACAGAATCTGAAATGCTTCGTACACCAA
ACTTTGGTCGCAAGTCGTTAAATGAAATTAAAGAAGTTTTGGCATGTATGGGATTACATCTTGGTATGGAAGTTCCTACG
TGGCCACCTGAGAACATTGATGATCTTGCTAAACGCTATGAAGATCAGTATTAA

Upstream 100 bases:

>100_bases
GTTTAATTGGGTGTTGTTTTTCGTTGTCGGAGGCTTCTCTGATGTTCACATTGTCCGTTACAATTGAATGGTAGCGGAAC
TAAAGAACGGGAATAAAAAT

Downstream 100 bases:

>100_bases
CGTTAAAATTTAAGGAGAAGGTTTATGCGCCATAGTAAGTCAGGTCGCAAGTTGAACCGGACTGCCAGTCACCGTAAAGC
AATGTTTGCAAATATGGCGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MIQKNWQELIKPNKVEFSKHDNPNISSVIVEPLERGFGLTLGNALRRVLLSSLRGAAITAVQIDGVLHEFSSIPGVREDV
TDIILNIKEIALRMREEGPKRIVVCKEGPGVLRAGDISTVGDMEILNPDHVICTLDEDAEIRMEFIVNTGKGYVPSDRNC
IDDARIGLIPVDSLYSPIRKVSYKVENTREGQVLDYDKLTLTVETNGAVNGEDALAFAARILQDQLALFINFKEPEKPTV
EESNSELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTESEMLRTPNFGRKSLNEIKEVLACMGLHLGMEVPT
WPPENIDDLAKRYEDQY

Sequences:

>Translated_337_residues
MIQKNWQELIKPNKVEFSKHDNPNISSVIVEPLERGFGLTLGNALRRVLLSSLRGAAITAVQIDGVLHEFSSIPGVREDV
TDIILNIKEIALRMREEGPKRIVVCKEGPGVLRAGDISTVGDMEILNPDHVICTLDEDAEIRMEFIVNTGKGYVPSDRNC
IDDARIGLIPVDSLYSPIRKVSYKVENTREGQVLDYDKLTLTVETNGAVNGEDALAFAARILQDQLALFINFKEPEKPTV
EESNSELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTESEMLRTPNFGRKSLNEIKEVLACMGLHLGMEVPT
WPPENIDDLAKRYEDQY
>Mature_337_residues
MIQKNWQELIKPNKVEFSKHDNPNISSVIVEPLERGFGLTLGNALRRVLLSSLRGAAITAVQIDGVLHEFSSIPGVREDV
TDIILNIKEIALRMREEGPKRIVVCKEGPGVLRAGDISTVGDMEILNPDHVICTLDEDAEIRMEFIVNTGKGYVPSDRNC
IDDARIGLIPVDSLYSPIRKVSYKVENTREGQVLDYDKLTLTVETNGAVNGEDALAFAARILQDQLALFINFKEPEKPTV
EESNSELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTESEMLRTPNFGRKSLNEIKEVLACMGLHLGMEVPT
WPPENIDDLAKRYEDQY

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=331, Percent_Identity=48.3383685800604, Blast_Score=303, Evalue=8e-84,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_BARHE (Q9FDC7)

Other databases:

- EMBL:   AF285111
- EMBL:   BX897699
- RefSeq:   YP_033811.1
- ProteinModelPortal:   Q9FDC7
- GeneID:   2864851
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH10270
- NMPDR:   fig|283166.1.peg.939
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q9FDC7
- ProtClustDB:   PRK05182
- BioCyc:   BHEN283166:BH10270-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37673; Mature: 37673

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKNWQELIKPNKVEFSKHDNPNISSVIVEPLERGFGLTLGNALRRVLLSSLRGAAITA
CCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
VQIDGVLHEFSSIPGVREDVTDIILNIKEIALRMREEGPKRIVVCKEGPGVLRAGDISTV
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCC
GDMEILNPDHVICTLDEDAEIRMEFIVNTGKGYVPSDRNCIDDARIGLIPVDSLYSPIRK
CCEEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VSYKVENTREGQVLDYDKLTLTVETNGAVNGEDALAFAARILQDQLALFINFKEPEKPTV
HHHHCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCC
EESNSELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTESEMLRTPNFGRKSL
CCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHH
NEIKEVLACMGLHLGMEVPTWPPENIDDLAKRYEDQY
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIQKNWQELIKPNKVEFSKHDNPNISSVIVEPLERGFGLTLGNALRRVLLSSLRGAAITA
CCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
VQIDGVLHEFSSIPGVREDVTDIILNIKEIALRMREEGPKRIVVCKEGPGVLRAGDISTV
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCC
GDMEILNPDHVICTLDEDAEIRMEFIVNTGKGYVPSDRNCIDDARIGLIPVDSLYSPIRK
CCEEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHH
VSYKVENTREGQVLDYDKLTLTVETNGAVNGEDALAFAARILQDQLALFINFKEPEKPTV
HHHHCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCC
EESNSELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTESEMLRTPNFGRKSL
CCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHH
NEIKEVLACMGLHLGMEVPTWPPENIDDLAKRYEDQY
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA