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The map label for this gene is nifS1 [H]

Identifier: 49475624

GI number: 49475624

Start: 964440

End: 965684

Strand: Reverse

Name: nifS1 [H]

Synonym: BH08610

Alternate gene names: 49475624

Gene position: 965684-964440 (Counterclockwise)

Preceding gene: 49475625

Following gene: 49475623

Centisome position: 50.01

GC content: 36.55

Gene sequence:

>1245_bases
ATGGAAAATAACGTACAAACATTAAGTTATAATGTAGAAGAAATTCGACGTGATTTTCCCCTTTTGCACCGTGATGTTTA
TGGAAAGCGATTAGCTTATCTTGACAGTGGTGCTTCTGCTCAAAAACCACAATCAGTGCTCAATGCAATGCATAATTGTT
ATCGACACAGTTACGCTAATGTGCATAGAGGAATATATTTTTTAGCGAATGCAGCAACACAATCTTATGAAAATGCCCGT
GAAACAGTTCGTATTTTTTTAAATGCTCAAACAGCTAAAGAAATTGTTTTTACAAAAAATGCAACAGAAGCCATTAATAC
TGTTGCTTATGGTTGGGCTATGTCCAAATTAAAGGAAGGTGATGAGATTGTTTTGACTATCATGGAACATCATTCAAATA
TTATTCCTTGGCATTTTCTTCGTGAACAAAAAGGTGTTAAGCTTGTTTTTGTCCCTGTTGATGAAAATGGTGTTCTCCAT
ATTGAAGATTTTGAAAAGGCTTTCAGCGAAAGAACGCGGCTTGTTACTCTTACTCATATGTCAAATATATTGGGAACTGT
ACCTCCTGTTAAAGAGATTATTAAACAAGCGCATCAAAATGGTATTCCTGTTCTTGTTGATGGTTCCCAAGGAGCTATAC
ATTTAACAGTTGATGTGCAAGATCTTGATTGTGATTGGTATGTGTTTACTGGTCATAAGCTTTATGGTCCTACGGGTATT
GGTGTTCTTTATGGTAAGGAACATAGGCTAGAAGAAATGCATCCTTTTCAAGGGGGAGGGGAAATGATCGAGGATGTGAC
AATTGATAAAGTTTCTTATAATGCTCCTCCTTACCGCTTTGAAGCGGGAACTCCTCCTATAGTTGAAGCTATTGGATTGG
CCGCTGCTATTGATTATATGCAAAAGAAGGATCGAAAAGCAATTCATGCCCATGAAATGGCACTTTTAACTTATGCGCAT
GAAAAACTAAAAACGGTTGATTCATTACGTATTTATGGTCATTCTCCTCATAAAGGAGCAATTATATCATTTGCAATTGA
AGGTATTCATGCACATGATATTGCGATGTTTTTGGATAGAAAAGGGGTTGCTATACGGGCAGGAACCCATTGTGCACAGC
CTTTATTACAACGCTTTGGTTTAACATCTATTTGTCGTGCATCATTAGCTATGTATAATACTTGTGAAGATATTGATCAG
TTAGTGGAAGCATTGAAAGAAACAAGGACATTTTTTAATGGCTGA

Upstream 100 bases:

>100_bases
TTCCGAACTCATTGATGATATCAAACAAGAGAATATCCAGACTATTTTGGAAAATATCACTGGCAAATGGTTAGAAAAAA
ATGTTTAAGGTAGAGAAGAA

Downstream 100 bases:

>100_bases
ACCAATTGAAGAGCGTCATTCTACAGAACCTGTTGAAACTGTAAGAGAAAATAAAAAATCTTACATATCAGCAATTCCGG
CAGATGAAATTGATCGTATG

Product: nitrogenase cofactor synthesis protein nifS

Products: NA

Alternate protein names: Selenocysteine beta-lyase; SCL; Selenocysteine lyase; Selenocysteine reductase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MENNVQTLSYNVEEIRRDFPLLHRDVYGKRLAYLDSGASAQKPQSVLNAMHNCYRHSYANVHRGIYFLANAATQSYENAR
ETVRIFLNAQTAKEIVFTKNATEAINTVAYGWAMSKLKEGDEIVLTIMEHHSNIIPWHFLREQKGVKLVFVPVDENGVLH
IEDFEKAFSERTRLVTLTHMSNILGTVPPVKEIIKQAHQNGIPVLVDGSQGAIHLTVDVQDLDCDWYVFTGHKLYGPTGI
GVLYGKEHRLEEMHPFQGGGEMIEDVTIDKVSYNAPPYRFEAGTPPIVEAIGLAAAIDYMQKKDRKAIHAHEMALLTYAH
EKLKTVDSLRIYGHSPHKGAIISFAIEGIHAHDIAMFLDRKGVAIRAGTHCAQPLLQRFGLTSICRASLAMYNTCEDIDQ
LVEALKETRTFFNG

Sequences:

>Translated_414_residues
MENNVQTLSYNVEEIRRDFPLLHRDVYGKRLAYLDSGASAQKPQSVLNAMHNCYRHSYANVHRGIYFLANAATQSYENAR
ETVRIFLNAQTAKEIVFTKNATEAINTVAYGWAMSKLKEGDEIVLTIMEHHSNIIPWHFLREQKGVKLVFVPVDENGVLH
IEDFEKAFSERTRLVTLTHMSNILGTVPPVKEIIKQAHQNGIPVLVDGSQGAIHLTVDVQDLDCDWYVFTGHKLYGPTGI
GVLYGKEHRLEEMHPFQGGGEMIEDVTIDKVSYNAPPYRFEAGTPPIVEAIGLAAAIDYMQKKDRKAIHAHEMALLTYAH
EKLKTVDSLRIYGHSPHKGAIISFAIEGIHAHDIAMFLDRKGVAIRAGTHCAQPLLQRFGLTSICRASLAMYNTCEDIDQ
LVEALKETRTFFNG
>Mature_414_residues
MENNVQTLSYNVEEIRRDFPLLHRDVYGKRLAYLDSGASAQKPQSVLNAMHNCYRHSYANVHRGIYFLANAATQSYENAR
ETVRIFLNAQTAKEIVFTKNATEAINTVAYGWAMSKLKEGDEIVLTIMEHHSNIIPWHFLREQKGVKLVFVPVDENGVLH
IEDFEKAFSERTRLVTLTHMSNILGTVPPVKEIIKQAHQNGIPVLVDGSQGAIHLTVDVQDLDCDWYVFTGHKLYGPTGI
GVLYGKEHRLEEMHPFQGGGEMIEDVTIDKVSYNAPPYRFEAGTPPIVEAIGLAAAIDYMQKKDRKAIHAHEMALLTYAH
EKLKTVDSLRIYGHSPHKGAIISFAIEGIHAHDIAMFLDRKGVAIRAGTHCAQPLLQRFGLTSICRASLAMYNTCEDIDQ
LVEALKETRTFFNG

Specific function: Cysteine desulfurases mobilize the sulfur from L- cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=25.2551020408163, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI156713448, Length=432, Percent_Identity=25.6944444444444, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=47.9899497487437, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=40.6565656565657, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI48994898, Length=354, Percent_Identity=26.5536723163842, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=350, Percent_Identity=24.8571428571429, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI193211090, Length=377, Percent_Identity=24.6684350132626, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=25.9351620947631, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20129463, Length=351, Percent_Identity=26.4957264957265, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7; =4.4.1.16 [H]

Molecular weight: Translated: 46540; Mature: 46540

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNVQTLSYNVEEIRRDFPLLHRDVYGKRLAYLDSGASAQKPQSVLNAMHNCYRHSYAN
CCCCCEEEEECHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VHRGIYFLANAATQSYENARETVRIFLNAQTAKEIVFTKNATEAINTVAYGWAMSKLKEG
HHCCEEEEEHHHHHHHHHHHHHEEEEEECCCHHHEEEECCHHHHHHHHHHHHHHHHHCCC
DEIVLTIMEHHSNIIPWHFLREQKGVKLVFVPVDENGVLHIEDFEKAFSERTRLVTLTHM
CCEEEEEEHHCCCCCCHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCEEEEEHHH
SNILGTVPPVKEIIKQAHQNGIPVLVDGSQGAIHLTVDVQDLDCDWYVFTGHKLYGPTGI
HHHHCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEECCCCEEEEECCEEECCCCC
GVLYGKEHRLEEMHPFQGGGEMIEDVTIDKVSYNAPPYRFEAGTPPIVEAIGLAAAIDYM
EEEECCCHHHHHCCCCCCCCHHHHHEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHH
QKKDRKAIHAHEMALLTYAHEKLKTVDSLRIYGHSPHKGAIISFAIEGIHAHDIAMFLDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCHHHHHHHHCC
KGVAIRAGTHCAQPLLQRFGLTSICRASLAMYNTCEDIDQLVEALKETRTFFNG
CCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENNVQTLSYNVEEIRRDFPLLHRDVYGKRLAYLDSGASAQKPQSVLNAMHNCYRHSYAN
CCCCCEEEEECHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VHRGIYFLANAATQSYENARETVRIFLNAQTAKEIVFTKNATEAINTVAYGWAMSKLKEG
HHCCEEEEEHHHHHHHHHHHHHEEEEEECCCHHHEEEECCHHHHHHHHHHHHHHHHHCCC
DEIVLTIMEHHSNIIPWHFLREQKGVKLVFVPVDENGVLHIEDFEKAFSERTRLVTLTHM
CCEEEEEEHHCCCCCCHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHCCCEEEEEHHH
SNILGTVPPVKEIIKQAHQNGIPVLVDGSQGAIHLTVDVQDLDCDWYVFTGHKLYGPTGI
HHHHCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEECCCCEEEEECCEEECCCCC
GVLYGKEHRLEEMHPFQGGGEMIEDVTIDKVSYNAPPYRFEAGTPPIVEAIGLAAAIDYM
EEEECCCHHHHHCCCCCCCCHHHHHEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHH
QKKDRKAIHAHEMALLTYAHEKLKTVDSLRIYGHSPHKGAIISFAIEGIHAHDIAMFLDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEECCCCHHHHHHHHCC
KGVAIRAGTHCAQPLLQRFGLTSICRASLAMYNTCEDIDQLVEALKETRTFFNG
CCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA