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The map label for this gene is paaF [C]

Identifier: 49475616

GI number: 49475616

Start: 957953

End: 958999

Strand: Reverse

Name: paaF [C]

Synonym: BH08520

Alternate gene names: 49475616

Gene position: 958999-957953 (Counterclockwise)

Preceding gene: 49475617

Following gene: 49475615

Centisome position: 49.66

GC content: 35.63

Gene sequence:

>1047_bases
ATGCGAATTGACTTTGGGGCAGGTGATGAGATTTCTTTTATAAAAAAAGGGCATGCTGGTGTTATAAAGCTCGCACGCTC
TTCAGCGTTAAACGCTCTTAATGGGCGAATGGTTTCAGCTTTAAAAAAAGCTCTGAGTGTATGGGAAACAGATGATGATG
TTTCTTGTGTTTTAATTGAAGGGGAGGGACGTGCTTTTTGTGCTGGTGGGGATGTTGTAGAAATCTACCATATGGAAGAA
AAGATTTCTTCTTACCAATATTTTAATGATGAATATCGCTTAAATGCTTATATTAAACATTTCCGAAAACCTTACATTTC
TTTTTTAAATGGTATTTGGATGGGGGGAGGGGTAGGTATTTCTATATATGGTTCACACCGAATTGTGACAGAAAATACAC
TCTTTGCTATGCCAGAAAGTGCCATTGGATTTTTTCCAGATGTTGGTGCAAGCTTTTTCTTACCATCTCTTCCCGATCAC
TTTGGCATTTATCTTGCATTGACCGGCGCGCGCATAAAATGGGGAGATTGCTTAAACTTAGGGTTAGCAACACATGCTGT
TCCTGAAATTGAATTAGAATGCATTAGAAAAGCTATTATTGAGCAGGGAAATCCTCAATTAGCTTTAGAAGAGCACGCCA
TCACAAAAGATTATGAAACAAGTCATGAGATACGCAGTGTTATAAATGCTTGTTTTGGTACACATACATTAGAAGAATGT
ATGGAACTCCTTTATAAAAAAAGTAATGAAGGACTTTTTTTTGCTAGAGAATGTTATGATATTTTGCAATCACGTTCTCC
GACAAGTTTAAAAGTTATTTGGAAACAAATGAAACAAAATTCATCTCAAACTTTAGAAGAGTGTATGAAAATTGAAAACC
GCATTGCACATCATATGATTAATTCACATGATTTTCGTGAAGGTGTACGTGCAATGTTAATTGATAAGGACAAAACACCA
AAATGGCAGCCAGATAAACTGTCGAATATAACAGATGAAATGATAGATGCTTACTTCCAACCTGTTGAAAAAGAATTATT
ATTTTGA

Upstream 100 bases:

>100_bases
AACAGGTATTTATATGTATTCTATTGGTGCTATCATGCAAATATTTCCTCAATTAGGAATAAAAAAACAATTAGATAGAT
AGTTTTAGGAAGAGTATGAG

Downstream 100 bases:

>100_bases
CCAATAACTCCCAAAAAAAGATTTCAAAAATCAATTTAATTTATAACTGTTATTTAAGATTTTTGGCGCTTATCTGCTTA
GGTTTAGGTGTTTTTTACTG

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRIDFGAGDEISFIKKGHAGVIKLARSSALNALNGRMVSALKKALSVWETDDDVSCVLIEGEGRAFCAGGDVVEIYHMEE
KISSYQYFNDEYRLNAYIKHFRKPYISFLNGIWMGGGVGISIYGSHRIVTENTLFAMPESAIGFFPDVGASFFLPSLPDH
FGIYLALTGARIKWGDCLNLGLATHAVPEIELECIRKAIIEQGNPQLALEEHAITKDYETSHEIRSVINACFGTHTLEEC
MELLYKKSNEGLFFARECYDILQSRSPTSLKVIWKQMKQNSSQTLEECMKIENRIAHHMINSHDFREGVRAMLIDKDKTP
KWQPDKLSNITDEMIDAYFQPVEKELLF

Sequences:

>Translated_348_residues
MRIDFGAGDEISFIKKGHAGVIKLARSSALNALNGRMVSALKKALSVWETDDDVSCVLIEGEGRAFCAGGDVVEIYHMEE
KISSYQYFNDEYRLNAYIKHFRKPYISFLNGIWMGGGVGISIYGSHRIVTENTLFAMPESAIGFFPDVGASFFLPSLPDH
FGIYLALTGARIKWGDCLNLGLATHAVPEIELECIRKAIIEQGNPQLALEEHAITKDYETSHEIRSVINACFGTHTLEEC
MELLYKKSNEGLFFARECYDILQSRSPTSLKVIWKQMKQNSSQTLEECMKIENRIAHHMINSHDFREGVRAMLIDKDKTP
KWQPDKLSNITDEMIDAYFQPVEKELLF
>Mature_348_residues
MRIDFGAGDEISFIKKGHAGVIKLARSSALNALNGRMVSALKKALSVWETDDDVSCVLIEGEGRAFCAGGDVVEIYHMEE
KISSYQYFNDEYRLNAYIKHFRKPYISFLNGIWMGGGVGISIYGSHRIVTENTLFAMPESAIGFFPDVGASFFLPSLPDH
FGIYLALTGARIKWGDCLNLGLATHAVPEIELECIRKAIIEQGNPQLALEEHAITKDYETSHEIRSVINACFGTHTLEEC
MELLYKKSNEGLFFARECYDILQSRSPTSLKVIWKQMKQNSSQTLEECMKIENRIAHHMINSHDFREGVRAMLIDKDKTP
KWQPDKLSNITDEMIDAYFQPVEKELLF

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=353, Percent_Identity=39.6600566572238, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI37594469, Length=308, Percent_Identity=38.6363636363636, Blast_Score=207, Evalue=8e-54,
Organism=Homo sapiens, GI194097323, Length=235, Percent_Identity=27.2340425531915, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1787659, Length=169, Percent_Identity=28.4023668639053, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=362, Percent_Identity=35.6353591160221, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25144157, Length=362, Percent_Identity=35.6353591160221, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=26.7489711934156, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=187, Percent_Identity=28.3422459893048, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=357, Percent_Identity=35.5742296918768, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28571729, Length=328, Percent_Identity=39.0243902439024, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI28571730, Length=328, Percent_Identity=39.0243902439024, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=27.3858921161826, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=27.3858921161826, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 39395; Mature: 39395

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDFGAGDEISFIKKGHAGVIKLARSSALNALNGRMVSALKKALSVWETDDDVSCVLIE
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GEGRAFCAGGDVVEIYHMEEKISSYQYFNDEYRLNAYIKHFRKPYISFLNGIWMGGGVGI
CCCCEEECCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SIYGSHRIVTENTLFAMPESAIGFFPDVGASFFLPSLPDHFGIYLALTGARIKWGDCLNL
EEECCEEEEECCCEEECCCHHCCCCCCCCCHHCCCCCCCCCEEEEEEECCEEECCCHHHC
GLATHAVPEIELECIRKAIIEQGNPQLALEEHAITKDYETSHEIRSVINACFGTHTLEEC
CHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHH
MELLYKKSNEGLFFARECYDILQSRSPTSLKVIWKQMKQNSSQTLEECMKIENRIAHHMI
HHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NSHDFREGVRAMLIDKDKTPKWQPDKLSNITDEMIDAYFQPVEKELLF
CCHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDFGAGDEISFIKKGHAGVIKLARSSALNALNGRMVSALKKALSVWETDDDVSCVLIE
CCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GEGRAFCAGGDVVEIYHMEEKISSYQYFNDEYRLNAYIKHFRKPYISFLNGIWMGGGVGI
CCCCEEECCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SIYGSHRIVTENTLFAMPESAIGFFPDVGASFFLPSLPDHFGIYLALTGARIKWGDCLNL
EEECCEEEEECCCEEECCCHHCCCCCCCCCHHCCCCCCCCCEEEEEEECCEEECCCHHHC
GLATHAVPEIELECIRKAIIEQGNPQLALEEHAITKDYETSHEIRSVINACFGTHTLEEC
CHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHH
MELLYKKSNEGLFFARECYDILQSRSPTSLKVIWKQMKQNSSQTLEECMKIENRIAHHMI
HHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
NSHDFREGVRAMLIDKDKTPKWQPDKLSNITDEMIDAYFQPVEKELLF
CCHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA