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The map label for this gene is pyrB

Identifier: 49475585

GI number: 49475585

Start: 925912

End: 926877

Strand: Reverse

Name: pyrB

Synonym: BH08200

Alternate gene names: 49475585

Gene position: 926877-925912 (Counterclockwise)

Preceding gene: 49475587

Following gene: 49475584

Centisome position: 48.0

GC content: 40.58

Gene sequence:

>966_bases
ATGACTCAAAATACTTTTTTTTCCCTTTTCCCCCACCAACATCTGTTAGGTATTAAAGATCTAAGTGTGCAAGACCTTAC
CATATTGCTTGATCGAGCAAATGCGAATGTTCCTTTCTTAAAAAAAAGTGATAAAAAACAATCCATATTGCATGGGCGTA
CGCAAATTAATCTCTTCTTTGAAGCTTCTACACGAACACAATCTTCATTTGAATTGGCCGGCAAACGGTTAGGAGCAGAC
GTGATGAGTATGGCTATTGGCAATTCATCTGTAAAAAAGGGGGAAACATTGGTTGATACAGCCACAACCCTCAATGCGAT
GAAGCCAGATATATTGGTCATTCGTCATAGTTGTGCCGGAGCAGCTGCTTTGTTAGCGCAAAAAGTTGACTGTTGTGTCA
TTAATGCAGGTGATGGTGCACATGAACATCCAACGCAGGCTTTATTAGATGCTCTGACCATTCAAAGAACAAAAGGCCGT
ATTGAAGGATTAACTGTTGCAATTTGTGGTGATATCTTGCATTCACGTGTCGCACGTTCCAACATCCTCAGTCTCAACGC
TTTAGGAGCATGTGTTCGTGTTATCGCTCCTTCAACACTTTTACCAGCAAGCATCGCAGATATGAGCGTTGAAGTTTATA
ACACCATGAAAGAAGGTCTTAAAGGTGCTGATGTCATCATGATGCTGCGCTTACAACAAGAACGTATGACAGGTTCCTTT
ATTCCCTCCATTCGTGAATATTTTCATTATTTCGGCTTACATAAAGAGAATTTAGCTTATGCTAAAAGTGATTGTATTAT
TCTACATCCTGGCCCAATAAACCGTGGTGTAGAAATCGCATCTGATATCGCAGATGGACCACAAAGCATGATTCATACAC
AAGTCGAAATGGGCATTGCTGTGCGTATGGCTGTCATGGAAGCTTTATTAGATTCACGCCTTAAGGCAAGTGGAGAAAAA
AAATGA

Upstream 100 bases:

>100_bases
TCATATCATAGACTATTTGTTTTTTTGCAGTTAAAAAATATTTATCATATCCAAACAAATGCTTGTTTAATCTCTTTAGA
GAGAGATAGAAAAGAATGCT

Downstream 100 bases:

>100_bases
CAAAGCCAATTGTTTTTCAAAACGCTCGTATTATTGATCCTTCCCGTACAATCGATGAAATTGGTACCGTTATTGTTGAA
AATGGACTGATTATAGCCGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTQNTFFSLFPHQHLLGIKDLSVQDLTILLDRANANVPFLKKSDKKQSILHGRTQINLFFEASTRTQSSFELAGKRLGAD
VMSMAIGNSSVKKGETLVDTATTLNAMKPDILVIRHSCAGAAALLAQKVDCCVINAGDGAHEHPTQALLDALTIQRTKGR
IEGLTVAICGDILHSRVARSNILSLNALGACVRVIAPSTLLPASIADMSVEVYNTMKEGLKGADVIMMLRLQQERMTGSF
IPSIREYFHYFGLHKENLAYAKSDCIILHPGPINRGVEIASDIADGPQSMIHTQVEMGIAVRMAVMEALLDSRLKASGEK
K

Sequences:

>Translated_321_residues
MTQNTFFSLFPHQHLLGIKDLSVQDLTILLDRANANVPFLKKSDKKQSILHGRTQINLFFEASTRTQSSFELAGKRLGAD
VMSMAIGNSSVKKGETLVDTATTLNAMKPDILVIRHSCAGAAALLAQKVDCCVINAGDGAHEHPTQALLDALTIQRTKGR
IEGLTVAICGDILHSRVARSNILSLNALGACVRVIAPSTLLPASIADMSVEVYNTMKEGLKGADVIMMLRLQQERMTGSF
IPSIREYFHYFGLHKENLAYAKSDCIILHPGPINRGVEIASDIADGPQSMIHTQVEMGIAVRMAVMEALLDSRLKASGEK
K
>Mature_320_residues
TQNTFFSLFPHQHLLGIKDLSVQDLTILLDRANANVPFLKKSDKKQSILHGRTQINLFFEASTRTQSSFELAGKRLGADV
MSMAIGNSSVKKGETLVDTATTLNAMKPDILVIRHSCAGAAALLAQKVDCCVINAGDGAHEHPTQALLDALTIQRTKGRI
EGLTVAICGDILHSRVARSNILSLNALGACVRVIAPSTLLPASIADMSVEVYNTMKEGLKGADVIMMLRLQQERMTGSFI
PSIREYFHYFGLHKENLAYAKSDCIILHPGPINRGVEIASDIADGPQSMIHTQVEMGIAVRMAVMEALLDSRLKASGEKK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=33.7662337662338, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=35.3503184713376, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1790703, Length=237, Percent_Identity=30.8016877637131, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=310, Percent_Identity=35.8064516129032, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=32.475884244373, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=35.2727272727273, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_BARHE (Q6G3F3)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033626.1
- ProteinModelPortal:   Q6G3F3
- SMR:   Q6G3F3
- GeneID:   2864779
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH08200
- NMPDR:   fig|283166.1.peg.754
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q6G3F3
- ProtClustDB:   PRK00856
- BioCyc:   BHEN283166:BH08200-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34849; Mature: 34718

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNTFFSLFPHQHLLGIKDLSVQDLTILLDRANANVPFLKKSDKKQSILHGRTQINLFF
CCCCCEEEECCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMSMAIGNSSVKKGETLVDTATTLNAMKPDILVIRHSCAG
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCH
AAALLAQKVDCCVINAGDGAHEHPTQALLDALTIQRTKGRIEGLTVAICGDILHSRVARS
HHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
NILSLNALGACVRVIAPSTLLPASIADMSVEVYNTMKEGLKGADVIMMLRLQQERMTGSF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
IPSIREYFHYFGLHKENLAYAKSDCIILHPGPINRGVEIASDIADGPQSMIHTQVEMGIA
HHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDSRLKASGEKK
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQNTFFSLFPHQHLLGIKDLSVQDLTILLDRANANVPFLKKSDKKQSILHGRTQINLFF
CCCCEEEECCCHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMSMAIGNSSVKKGETLVDTATTLNAMKPDILVIRHSCAG
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCH
AAALLAQKVDCCVINAGDGAHEHPTQALLDALTIQRTKGRIEGLTVAICGDILHSRVARS
HHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHH
NILSLNALGACVRVIAPSTLLPASIADMSVEVYNTMKEGLKGADVIMMLRLQQERMTGSF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
IPSIREYFHYFGLHKENLAYAKSDCIILHPGPINRGVEIASDIADGPQSMIHTQVEMGIA
HHHHHHHHHHHCCCCCCCCEECCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDSRLKASGEKK
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA