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The map label for this gene is ppnK [H]

Identifier: 49475436

GI number: 49475436

Start: 761433

End: 762206

Strand: Reverse

Name: ppnK [H]

Synonym: BH06480

Alternate gene names: 49475436

Gene position: 762206-761433 (Counterclockwise)

Preceding gene: 49475443

Following gene: 49475435

Centisome position: 39.47

GC content: 40.57

Gene sequence:

>774_bases
ATGACTCAATTACCAAGCCGCTTTCATTTTATTTCTGCGGAAACTGAGAACGCTATTAAAGCGACCAATAAATTAATTTC
CGTTTACGGTCACTATTCTTTGGAAGAAACTGATGTCGTTGTTGCAATTGGTGGCGATGGAACAATGTTACAAACAGTAC
ACAATGTTATGAACACTGGAAAACCTATTTATGGCATGAATCAAGGCGCTGTAGGATTTCTTATGAATGAATTTCATGAA
GAAAAATTGCCAAATCGCATTGCTGTCGCACATAAAAAGGAGATTCATCCCCTGCGTATGATTGCAAAAGCTGAGTGTCA
AGGAACTATTGAAGCACTCGCCATCAATGAAGTATCTCTCTTTCGTCAATCTTACCAGGCAGCAAAAATTCGTATCAGCA
TTGATAACAACGTGCGTATGGAACAATTAAGTTGCGATGGTATCCTCGTTGCCACACCAGCAGGATCTACCGCATATAAT
TTATCAGCGCAAGGACCTATTTTGCCTCTCATGGCTCCCCTTATGGCGCTTACTCCCGTTAGTCCTTTTCGTCCTCGTCG
CTGGCATGGAGCATTGCTTCCTAACACGGCAATAGTACGCTTTGATATGCTCGAATCTGACAAACGTCCTGTAAATGCAG
CAGCCGATAATGTTGAAGTTAAATCTGTCTATTCAGTTACTATTTCAACGGCAAAAGAAGTAACAGTCTCAATCCTTTTT
GACTCTAACCATTCATGGGATGAACGTATTTTATCAGAACAATTCCTCTATTAA

Upstream 100 bases:

>100_bases
ACAAAATTCTCTTCTTTTTATAAAAACAGCTTTTATTACAACTTCAAAACTCTCAAAAAGTAAGCTACAAAATAAATTGC
AAATATCAGGATAACTTTTA

Downstream 100 bases:

>100_bases
TTATTGTATTATGCTCTCTATGCGTGTATGTCCGTTATAGTTGACTTTTTTATGAAGGCATCTTACCCCTCTCTATAATG
TAGGATACTTAATTTACTAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTQLPSRFHFISAETENAIKATNKLISVYGHYSLEETDVVVAIGGDGTMLQTVHNVMNTGKPIYGMNQGAVGFLMNEFHE
EKLPNRIAVAHKKEIHPLRMIAKAECQGTIEALAINEVSLFRQSYQAAKIRISIDNNVRMEQLSCDGILVATPAGSTAYN
LSAQGPILPLMAPLMALTPVSPFRPRRWHGALLPNTAIVRFDMLESDKRPVNAAADNVEVKSVYSVTISTAKEVTVSILF
DSNHSWDERILSEQFLY

Sequences:

>Translated_257_residues
MTQLPSRFHFISAETENAIKATNKLISVYGHYSLEETDVVVAIGGDGTMLQTVHNVMNTGKPIYGMNQGAVGFLMNEFHE
EKLPNRIAVAHKKEIHPLRMIAKAECQGTIEALAINEVSLFRQSYQAAKIRISIDNNVRMEQLSCDGILVATPAGSTAYN
LSAQGPILPLMAPLMALTPVSPFRPRRWHGALLPNTAIVRFDMLESDKRPVNAAADNVEVKSVYSVTISTAKEVTVSILF
DSNHSWDERILSEQFLY
>Mature_256_residues
TQLPSRFHFISAETENAIKATNKLISVYGHYSLEETDVVVAIGGDGTMLQTVHNVMNTGKPIYGMNQGAVGFLMNEFHEE
KLPNRIAVAHKKEIHPLRMIAKAECQGTIEALAINEVSLFRQSYQAAKIRISIDNNVRMEQLSCDGILVATPAGSTAYNL
SAQGPILPLMAPLMALTPVSPFRPRRWHGALLPNTAIVRFDMLESDKRPVNAAADNVEVKSVYSVTISTAKEVTVSILFD
SNHSWDERILSEQFLY

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=151, Percent_Identity=32.4503311258278, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325068, Length=192, Percent_Identity=31.7708333333333, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322509, Length=224, Percent_Identity=28.125, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 28427; Mature: 28296

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLPSRFHFISAETENAIKATNKLISVYGHYSLEETDVVVAIGGDGTMLQTVHNVMNTG
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KPIYGMNQGAVGFLMNEFHEEKLPNRIAVAHKKEIHPLRMIAKAECQGTIEALAINEVSL
CCEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHH
FRQSYQAAKIRISIDNNVRMEQLSCDGILVATPAGSTAYNLSAQGPILPLMAPLMALTPV
HHHCCCEEEEEEEECCCCEEEEECCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCC
SPFRPRRWHGALLPNTAIVRFDMLESDKRPVNAAADNVEVKSVYSVTISTAKEVTVSILF
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEE
DSNHSWDERILSEQFLY
CCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TQLPSRFHFISAETENAIKATNKLISVYGHYSLEETDVVVAIGGDGTMLQTVHNVMNTG
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHCC
KPIYGMNQGAVGFLMNEFHEEKLPNRIAVAHKKEIHPLRMIAKAECQGTIEALAINEVSL
CCEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHH
FRQSYQAAKIRISIDNNVRMEQLSCDGILVATPAGSTAYNLSAQGPILPLMAPLMALTPV
HHHCCCEEEEEEEECCCCEEEEECCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCC
SPFRPRRWHGALLPNTAIVRFDMLESDKRPVNAAADNVEVKSVYSVTISTAKEVTVSILF
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEEE
DSNHSWDERILSEQFLY
CCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]