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The map label for this gene is dacA1 [H]

Identifier: 49475404

GI number: 49475404

Start: 722725

End: 724074

Strand: Reverse

Name: dacA1 [H]

Synonym: BH06160

Alternate gene names: 49475404

Gene position: 724074-722725 (Counterclockwise)

Preceding gene: 49475407

Following gene: 49475403

Centisome position: 37.5

GC content: 38.44

Gene sequence:

>1350_bases
GTGTATATTGACTGCCAAAAAATTGTGCGTTGCTATAAAAAAATAGCAATTGCGTTTGTTGTTTTAGCTCTTTCTTACTC
TTGGGCATTCGCTACTCCTCAAGAAGCCTATCCTGATAAGTATGCTGCTATCGTTATAGATGCAAATACAGGAAAAACTC
TATTTCAAGCCAATGCAACATTAAAACGTTATCCCGCTTCTTTGACAAAAATGATGACGCTTTATCTGCTTTTTGAAGCT
ATGCATATGCGTCGTGTAACACCCAATACCCCAATCCTTGTCTCACGGAATGCAGCAACACGCCCACCCACAAAACTTGG
ACTTAAAACAGGACAAACAATTTCTGCACAAGAAGCCGCTAAAGCACTCATTACACGATCAGCAAATGATGTTGCTACTG
CGATTGCCGAATATCTTGGTGGCAATGAAAAAAAATTCGCACGAATGATGACAGCTAAAGCTCGCAAACTTGGTATGATA
AATACTCATTTCGCAAATGCTTCAGGACTTCCGGATGTGCGCAATTATTCTACAGCACGAGATATGGCTACTTTGTCATT
GGCCTTACGCAAACATTTTCCACAACAGTATAAATTGTTCAAAATAAAAAGTTTTATTTTCCATGGACATACCGTAAACA
CTCACAATAAATTAGTCAAAACAATGAAAGGCGTCGATGGAATTAAGACAGGATACACACAAATGTCAGGTTCTAATTTA
GCGACTTCTATGCGTATTGAAGGGCGCTCTATCGTTGCTGTCGTTATGGGAGGTAAATCCTCCACCGCACGCGATAAGCA
TATGGCCAGCTTATTGAGCCAATATTTACCCAAAGCAAGCCGCATGAAAAATGATGGACGTTTAATGGCTTCTGCACACT
ATAACTTACCCAATGGCGCTCAAACTCCTATACCACCTGTTAAAGCTGATCCAACCGATTCCGATGATGAAATTTCTACT
CTATTGACAGCATTTGCAGAACAATCTAGAAATTCCAATACAGCAATAGCCGCTGTAAACCAGGCAATTATACCAATCCC
TAACCCTCAAAAAGCTATATCTGTTAAAATTAATGAAATTGTTACGGCCTCTTTGTCCACAAACGCTGGATGGGCTATCC
AAATTGGTTCTCTTCCTAGTAAAAAACAAGCAGAAACTTTACTTTTAAAAGCAAAAAATAAAGCCTACTCCGCTTTAAAG
AACGCATCTTCACATATGCAACTTTTTGAAAAAAACAAACGTCGTTATTATCGTGCACGGTTTGTAGGATTTCAATCAAA
AAAAGCTGCATTTAATGCTTGTTCTCTATTAAAGAAAGCAAATTTTAACTGCTATACTGTAGCTTATTAA

Upstream 100 bases:

>100_bases
ACAAATTAGTAATATCATTCATATAAATTGTATCAACAAACTGCTGCGCAAAAAAGTTTTACAGATTTTTTAGTGTAAAT
GGAGAAATAGGATACTAAAA

Downstream 100 bases:

>100_bases
AGGTTTTTGAATTCTGCGTTAAGGATAGTACCATGACACATAATGCTCTTGCTTCATCCACTGAAAAAGAAAAATTATTG
AAGCAATCATTTCTTCTTTC

Product: penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MYIDCQKIVRCYKKIAIAFVVLALSYSWAFATPQEAYPDKYAAIVIDANTGKTLFQANATLKRYPASLTKMMTLYLLFEA
MHMRRVTPNTPILVSRNAATRPPTKLGLKTGQTISAQEAAKALITRSANDVATAIAEYLGGNEKKFARMMTAKARKLGMI
NTHFANASGLPDVRNYSTARDMATLSLALRKHFPQQYKLFKIKSFIFHGHTVNTHNKLVKTMKGVDGIKTGYTQMSGSNL
ATSMRIEGRSIVAVVMGGKSSTARDKHMASLLSQYLPKASRMKNDGRLMASAHYNLPNGAQTPIPPVKADPTDSDDEIST
LLTAFAEQSRNSNTAIAAVNQAIIPIPNPQKAISVKINEIVTASLSTNAGWAIQIGSLPSKKQAETLLLKAKNKAYSALK
NASSHMQLFEKNKRRYYRARFVGFQSKKAAFNACSLLKKANFNCYTVAY

Sequences:

>Translated_449_residues
MYIDCQKIVRCYKKIAIAFVVLALSYSWAFATPQEAYPDKYAAIVIDANTGKTLFQANATLKRYPASLTKMMTLYLLFEA
MHMRRVTPNTPILVSRNAATRPPTKLGLKTGQTISAQEAAKALITRSANDVATAIAEYLGGNEKKFARMMTAKARKLGMI
NTHFANASGLPDVRNYSTARDMATLSLALRKHFPQQYKLFKIKSFIFHGHTVNTHNKLVKTMKGVDGIKTGYTQMSGSNL
ATSMRIEGRSIVAVVMGGKSSTARDKHMASLLSQYLPKASRMKNDGRLMASAHYNLPNGAQTPIPPVKADPTDSDDEIST
LLTAFAEQSRNSNTAIAAVNQAIIPIPNPQKAISVKINEIVTASLSTNAGWAIQIGSLPSKKQAETLLLKAKNKAYSALK
NASSHMQLFEKNKRRYYRARFVGFQSKKAAFNACSLLKKANFNCYTVAY
>Mature_449_residues
MYIDCQKIVRCYKKIAIAFVVLALSYSWAFATPQEAYPDKYAAIVIDANTGKTLFQANATLKRYPASLTKMMTLYLLFEA
MHMRRVTPNTPILVSRNAATRPPTKLGLKTGQTISAQEAAKALITRSANDVATAIAEYLGGNEKKFARMMTAKARKLGMI
NTHFANASGLPDVRNYSTARDMATLSLALRKHFPQQYKLFKIKSFIFHGHTVNTHNKLVKTMKGVDGIKTGYTQMSGSNL
ATSMRIEGRSIVAVVMGGKSSTARDKHMASLLSQYLPKASRMKNDGRLMASAHYNLPNGAQTPIPPVKADPTDSDDEIST
LLTAFAEQSRNSNTAIAAVNQAIIPIPNPQKAISVKINEIVTASLSTNAGWAIQIGSLPSKKQAETLLLKAKNKAYSALK
NASSHMQLFEKNKRRYYRARFVGFQSKKAAFNACSLLKKANFNCYTVAY

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=274, Percent_Identity=34.3065693430657, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786851, Length=242, Percent_Identity=34.7107438016529, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1787062, Length=242, Percent_Identity=34.7107438016529, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082054, Length=226, Percent_Identity=28.3185840707965, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 49372; Mature: 49372

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIDCQKIVRCYKKIAIAFVVLALSYSWAFATPQEAYPDKYAAIVIDANTGKTLFQANAT
CEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEECCCCCEEEECCCH
LKRYPASLTKMMTLYLLFEAMHMRRVTPNTPILVSRNAATRPPTKLGLKTGQTISAQEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHH
KALITRSANDVATAIAEYLGGNEKKFARMMTAKARKLGMINTHFANASGLPDVRNYSTAR
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
DMATLSLALRKHFPQQYKLFKIKSFIFHGHTVNTHNKLVKTMKGVDGIKTGYTQMSGSNL
HHHHHHHHHHHHCCHHHHHHHHHHHHEECCEECHHHHHHHHHHCCCCHHCCCCCCCCCCC
ATSMRIEGRSIVAVVMGGKSSTARDKHMASLLSQYLPKASRMKNDGRLMASAHYNLPNGA
EEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCC
QTPIPPVKADPTDSDDEISTLLTAFAEQSRNSNTAIAAVNQAIIPIPNPQKAISVKINEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCEEEEEEHHE
VTASLSTNAGWAIQIGSLPSKKQAETLLLKAKNKAYSALKNASSHMQLFEKNKRRYYRAR
EEEEECCCCCEEEEECCCCCHHHHHHHHEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FVGFQSKKAAFNACSLLKKANFNCYTVAY
HHCCCCHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MYIDCQKIVRCYKKIAIAFVVLALSYSWAFATPQEAYPDKYAAIVIDANTGKTLFQANAT
CEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEECCCCCEEEECCCH
LKRYPASLTKMMTLYLLFEAMHMRRVTPNTPILVSRNAATRPPTKLGLKTGQTISAQEAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHH
KALITRSANDVATAIAEYLGGNEKKFARMMTAKARKLGMINTHFANASGLPDVRNYSTAR
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHH
DMATLSLALRKHFPQQYKLFKIKSFIFHGHTVNTHNKLVKTMKGVDGIKTGYTQMSGSNL
HHHHHHHHHHHHCCHHHHHHHHHHHHEECCEECHHHHHHHHHHCCCCHHCCCCCCCCCCC
ATSMRIEGRSIVAVVMGGKSSTARDKHMASLLSQYLPKASRMKNDGRLMASAHYNLPNGA
EEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCC
QTPIPPVKADPTDSDDEISTLLTAFAEQSRNSNTAIAAVNQAIIPIPNPQKAISVKINEI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCEEEEEEHHE
VTASLSTNAGWAIQIGSLPSKKQAETLLLKAKNKAYSALKNASSHMQLFEKNKRRYYRAR
EEEEECCCCCEEEEECCCCCHHHHHHHHEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FVGFQSKKAAFNACSLLKKANFNCYTVAY
HHCCCCHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]