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The map label for this gene is ispDF [H]

Identifier: 49475375

GI number: 49475375

Start: 682332

End: 683411

Strand: Reverse

Name: ispDF [H]

Synonym: BH05820

Alternate gene names: 49475375

Gene position: 683411-682332 (Counterclockwise)

Preceding gene: 49475376

Following gene: 49475374

Centisome position: 35.39

GC content: 40.93

Gene sequence:

>1080_bases
GTGCGTTGTTTTTGCCAAAATCCAGCCATAACAACGATTATCCTCGTTATTCATCCAGAGGACCGTCAAATCTGTGAACA
AGCTATTGCCGACTTTAAAGAGCAGCTCATCATCGTTGAAGGAGGAAATACTCGCCAAAAATCTACCTTACGTGGACTTC
AAGCACTTAGAAAGTTTAAGCCTAAATATGTACATATTCATGATGGTGCACGTCCCTTTGTCGAAAACAAGCTCCTCGAA
CAAATACACACCACCGTTACTCCTCAAGAAGGCGTTCTTCCCGTTCTCGCCGTCTGTGATACTCTCAAACGTATCAATAG
CACACACCATGTTTTAGAAACAATTCCACGCACACATCTTTATAGCGCACAAACTCCACAGTGTTTTCCTTTTGAACGCA
TCTTAGCAGCCCATGAAAAAGCAATAAAAACTTGCAAAAAAGAATTCACCGACGATTCTGCAATTGCTGAATGGTTTGGA
ATTTCTATGCGTACCATTCCTGGAAGCCCTCACAACATAAAGATTACATGGCATGAAGATTTGAATACCGCACATTTATA
TCTCCAGAAAAAAATGCAAATGTTTCCTGATATCCGTACCGGCAATGGTTATGATGTTCATTCTTTCGAAGAAGGAAATT
CTCTTATATTATGTGGCATAAAAATCCCTTTTCATAAAAAATTAAATGGACACTCTGATGCTGATGTCGCTCTTCATGCA
CTTACAGATGCTCTTCTCGCTACCCGGGGAGCAGGAGATATCGGCACACATTTTCCCCCCTCTGATCCTCAATGGCAAAA
TGTATCTTCAGAAATTTTTCTACGTCACGCTCTTGATATTGTTAAACAAGCAGGTGGTCGTATTGCCAATGTTGATATCA
CACTCATCGCTGAAGAACCTAAAATCGGCCCCTATCGTCATGCAATGGTAGGAAATCTTATGAACATGCTCACAATTTTA
CCCGATCGAATCTCTATAAAAGCCACAACAAATGAAAAACTTGGATTTATTGGTCGTGGCGAAGGCATTGCGGCTTTTGC
AACAGCAAACGTCCTTTATCCAGGAGAAATTCCCAAATGA

Upstream 100 bases:

>100_bases
AGTCATATTAGCCGCTGGACGCGGTAAAAGAGCAGGATCTCTTCCAAAAAAACCAAAACAATACCGACTCTTAGGACAAG
AGCCGGTTATTTGCCATACT

Downstream 100 bases:

>100_bases
CATGTTTTTGTGAAAAACAAGCACGTGAAGTCCTTACAGCTTGTCGCCAAAAAAGTTTGCTTTTAACAACTGTCGAGTCT
TGCACAGGAGGGCTCATTGC

Product: hypothetical protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MRCFCQNPAITTIILVIHPEDRQICEQAIADFKEQLIIVEGGNTRQKSTLRGLQALRKFKPKYVHIHDGARPFVENKLLE
QIHTTVTPQEGVLPVLAVCDTLKRINSTHHVLETIPRTHLYSAQTPQCFPFERILAAHEKAIKTCKKEFTDDSAIAEWFG
ISMRTIPGSPHNIKITWHEDLNTAHLYLQKKMQMFPDIRTGNGYDVHSFEEGNSLILCGIKIPFHKKLNGHSDADVALHA
LTDALLATRGAGDIGTHFPPSDPQWQNVSSEIFLRHALDIVKQAGGRIANVDITLIAEEPKIGPYRHAMVGNLMNMLTIL
PDRISIKATTNEKLGFIGRGEGIAAFATANVLYPGEIPK

Sequences:

>Translated_359_residues
MRCFCQNPAITTIILVIHPEDRQICEQAIADFKEQLIIVEGGNTRQKSTLRGLQALRKFKPKYVHIHDGARPFVENKLLE
QIHTTVTPQEGVLPVLAVCDTLKRINSTHHVLETIPRTHLYSAQTPQCFPFERILAAHEKAIKTCKKEFTDDSAIAEWFG
ISMRTIPGSPHNIKITWHEDLNTAHLYLQKKMQMFPDIRTGNGYDVHSFEEGNSLILCGIKIPFHKKLNGHSDADVALHA
LTDALLATRGAGDIGTHFPPSDPQWQNVSSEIFLRHALDIVKQAGGRIANVDITLIAEEPKIGPYRHAMVGNLMNMLTIL
PDRISIKATTNEKLGFIGRGEGIAAFATANVLYPGEIPK
>Mature_359_residues
MRCFCQNPAITTIILVIHPEDRQICEQAIADFKEQLIIVEGGNTRQKSTLRGLQALRKFKPKYVHIHDGARPFVENKLLE
QIHTTVTPQEGVLPVLAVCDTLKRINSTHHVLETIPRTHLYSAQTPQCFPFERILAAHEKAIKTCKKEFTDDSAIAEWFG
ISMRTIPGSPHNIKITWHEDLNTAHLYLQKKMQMFPDIRTGNGYDVHSFEEGNSLILCGIKIPFHKKLNGHSDADVALHA
LTDALLATRGAGDIGTHFPPSDPQWQNVSSEIFLRHALDIVKQAGGRIANVDITLIAEEPKIGPYRHAMVGNLMNMLTIL
PDRISIKATTNEKLGFIGRGEGIAAFATANVLYPGEIPK

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=152, Percent_Identity=46.7105263157895, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789104, Length=188, Percent_Identity=29.2553191489362, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 40048; Mature: 40048

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFCQNPAITTIILVIHPEDRQICEQAIADFKEQLIIVEGGNTRQKSTLRGLQALRKFK
CCEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCC
PKYVHIHDGARPFVENKLLEQIHTTVTPQEGVLPVLAVCDTLKRINSTHHVLETIPRTHL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCHHHC
YSAQTPQCFPFERILAAHEKAIKTCKKEFTDDSAIAEWFGISMRTIPGSPHNIKITWHED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCCCEEEEEECCC
LNTAHLYLQKKMQMFPDIRTGNGYDVHSFEEGNSLILCGIKIPFHKKLNGHSDADVALHA
CCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
LTDALLATRGAGDIGTHFPPSDPQWQNVSSEIFLRHALDIVKQAGGRIANVDITLIAEEP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
KIGPYRHAMVGNLMNMLTILPDRISIKATTNEKLGFIGRGEGIAAFATANVLYPGEIPK
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCEEEEEEECEECCCCCCC
>Mature Secondary Structure
MRCFCQNPAITTIILVIHPEDRQICEQAIADFKEQLIIVEGGNTRQKSTLRGLQALRKFK
CCEEECCCCEEEEEEEEECCHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHCC
PKYVHIHDGARPFVENKLLEQIHTTVTPQEGVLPVLAVCDTLKRINSTHHVLETIPRTHL
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCHHHC
YSAQTPQCFPFERILAAHEKAIKTCKKEFTDDSAIAEWFGISMRTIPGSPHNIKITWHED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCCCEEEEEECCC
LNTAHLYLQKKMQMFPDIRTGNGYDVHSFEEGNSLILCGIKIPFHKKLNGHSDADVALHA
CCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
LTDALLATRGAGDIGTHFPPSDPQWQNVSSEIFLRHALDIVKQAGGRIANVDITLIAEEP
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
KIGPYRHAMVGNLMNMLTILPDRISIKATTNEKLGFIGRGEGIAAFATANVLYPGEIPK
CCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCEEEEEEECEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA