Click here to switch to the map view.

The map label for this gene is glyS [H]

Identifier: 49475213

GI number: 49475213

Start: 476176

End: 478398

Strand: Reverse

Name: glyS [H]

Synonym: BH04150

Alternate gene names: 49475213

Gene position: 478398-476176 (Counterclockwise)

Preceding gene: 49475214

Following gene: 49475204

Centisome position: 24.77

GC content: 38.46

Gene sequence:

>2223_bases
ATGTCTGATCTTCTTCTTGAACTTTTCAGCGAAGAGATCCCTGCTCGTATGCAACGAAAAGCTGCTGCAGACCTCAAAAA
ATCTGTGACAGATCAACTTGTGAATGCAGGTTTAACCTATAAAGCTGCCCATGAATATTGGACACCTCGGCGGCTGACAT
TAAACATACGCGGTCTTTCTATACGTTCAAAAGATACCCATGAAGAACGCAAAGGACCCAGTACAAAATCTCCACAGCAC
GTAATTGATGGCTTTCTTCGTGCAACAGGATTGAGCGACATTTCTAAAGCACACATTGCACATGATCATAAAAAGGGTGA
TTTTTATATCGCTAAAATCGTCAAAAAAGGACGAAGTGCAGAAGAAATTATTGCTGATATTCTACCAGATATTATTCGTA
ACTTTCCATGGCCAAAATCTATGCGTTGGGGCAAAGATTCAGAAAAAAGTGGAGCTCTGAAGTGGATTCGATCTTTACAA
AACATTCTTTGCGTCTTTGGACCAGAAATTGGTGAAACACATGTTATCCCTTTTACAATTGGTTCTCTCAAAAGCAATAA
TTTAACCTATGGTCATCGCTTTTTAAGTGACGGAAAACCCCTCCAAGTTCGCCGCTTTGATGACTATGTTACCCAACTTG
AAATTCATAAAGTCATTTTGGATGCCGAAAGACGAAAGAATATTATTTTGGCTGATGCGCAAAATCTTTGTTTTGCAAAC
GGTTTAGAATTGGTTAAAGACAATGCTCTCTTAGAAGAAGTTGTAGGACTCATTGAGTGGCCTGTTGTACTTATGGGAAA
TTTTGACAAATCTTTCCTTAATATTCCTCCAGAAATTATTCGCCTAACCATTCGAGCCAATCAAAAGTGTTTCGTAACCC
GCAAACAAGGCGAAAAAATTAAACTTTCTCATCATTTTATTCTCGTTTCTAATATTCTTGCAAGTGACGAAGGCAAAGAA
ATTTCTAAGGGAAATAGTAAAGTTGTCTGCGCTCGTCTTTCCGATGCACTCTATTTTTGGCAAACAGACCAACATGACTT
GCCAGATATCAAATATTTGCAATCTTCTGCTAAAAAACTTGATCTCGATTTAAAAAAACCTCTCGATCAAAGAATGGCAC
AACTTGATCATTTAAATGTGACTTTTCATGCAAAATTAGGCACGCAAGGTGCAAGAGTAGAGCGAATCACTATTCTTGCA
CAACAAATTGCGCCTTTGGTGCACGCAGATCCTTTCTTAGCTAAGCGTGCAGCAGTGCTTGCAAAAGCTGATTTACAAAC
AGAAATTGTTGGAGAATTTCCGGAACTACAGGGGCTCATGGGACGAAAATATGCCCTTCTTCAAGGAGAAGATCCTCGCG
TAGCTGAAGCAATCGAAGACCATTATAAGCCACAAGGACCAGCAGATCGTATTCCGCAGGAGCCTCTTGCTATAGCAGTT
GCACTAGCAGATAAAATTGACATGCTCGTCGGTTTTTGGCTCATTAATGAAAAACCAAGCAGTTCAAAAGATCCCTATGC
ATTAAGACGAGCAGTATTAGGAGTTATCAGGCTTGTGCTTTTGCGTGACTGGAAAATCAATCTTATGCCATTATTTAATC
TGACAGCGAATCTTTTCTTACAACAAAAAACTCCATATGGGACATCTCGAAGAGAAGCTACGCAATCGCAGAATGTACTT
CCTGAAAAAACAGAACATATTTTATCGGATCTGTTATCTTTTTTTCATGAACGTTTGAAAATCTATTTAAAAGAAGAAGG
CGCTCGTTATGATGTTCTTGAAGCAGTCTTAAAAAAAGATGCTGACGATCTGTTGCTGATTGCACGTCATGTCGAAGCAC
TTATCGCCTTTATCAATACAAGTGATGGAGATAGCTTTTTAGCTGCTGTAAAACGTACCGTAAATATTCTTGAGGCTGAA
TCCCAAAAAGATTCAACAATAGAAGATGAAATTAACCCTGAACTCTTTATTGAAACAGAAGAAAAACAACTCTATCAAGC
AATCACTGAGATAGAGAAAACAGTTCATCACTCTATCAATGCAACAAATTTACCACACCTACTCAATACGCTAACACCAC
TGGGAAAATTTATAGACACATTTTTTGAGAAAGTATTGGTAAACGATAAAAATCCCCATGTTCGGACGAATCGTCTTGCT
CTTCTCAAACGTATTCATACTCTTACACAAAAAGCTGCGGATTTCTCAAAACTTGTAGTCTAA

Upstream 100 bases:

>100_bases
CAGAGTTATATTCTTCGCGTTCGTGATCTTGCACGCCGCTGTGGGGAAGCCTTCTTACTCACTGAAGCTGGGAAAATACA
CACTAACGGAGAAAAAACTA

Downstream 100 bases:

>100_bases
ATTTAGACTCTGTCTACCCCTTAAAATTTGAGAGTAAAGATAGATAAATGAAAGAATTCTATTTGTAAAAAATAGGAATG
TTATCATAGGGAAATAGAAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MSDLLLELFSEEIPARMQRKAAADLKKSVTDQLVNAGLTYKAAHEYWTPRRLTLNIRGLSIRSKDTHEERKGPSTKSPQH
VIDGFLRATGLSDISKAHIAHDHKKGDFYIAKIVKKGRSAEEIIADILPDIIRNFPWPKSMRWGKDSEKSGALKWIRSLQ
NILCVFGPEIGETHVIPFTIGSLKSNNLTYGHRFLSDGKPLQVRRFDDYVTQLEIHKVILDAERRKNIILADAQNLCFAN
GLELVKDNALLEEVVGLIEWPVVLMGNFDKSFLNIPPEIIRLTIRANQKCFVTRKQGEKIKLSHHFILVSNILASDEGKE
ISKGNSKVVCARLSDALYFWQTDQHDLPDIKYLQSSAKKLDLDLKKPLDQRMAQLDHLNVTFHAKLGTQGARVERITILA
QQIAPLVHADPFLAKRAAVLAKADLQTEIVGEFPELQGLMGRKYALLQGEDPRVAEAIEDHYKPQGPADRIPQEPLAIAV
ALADKIDMLVGFWLINEKPSSSKDPYALRRAVLGVIRLVLLRDWKINLMPLFNLTANLFLQQKTPYGTSRREATQSQNVL
PEKTEHILSDLLSFFHERLKIYLKEEGARYDVLEAVLKKDADDLLLIARHVEALIAFINTSDGDSFLAAVKRTVNILEAE
SQKDSTIEDEINPELFIETEEKQLYQAITEIEKTVHHSINATNLPHLLNTLTPLGKFIDTFFEKVLVNDKNPHVRTNRLA
LLKRIHTLTQKAADFSKLVV

Sequences:

>Translated_740_residues
MSDLLLELFSEEIPARMQRKAAADLKKSVTDQLVNAGLTYKAAHEYWTPRRLTLNIRGLSIRSKDTHEERKGPSTKSPQH
VIDGFLRATGLSDISKAHIAHDHKKGDFYIAKIVKKGRSAEEIIADILPDIIRNFPWPKSMRWGKDSEKSGALKWIRSLQ
NILCVFGPEIGETHVIPFTIGSLKSNNLTYGHRFLSDGKPLQVRRFDDYVTQLEIHKVILDAERRKNIILADAQNLCFAN
GLELVKDNALLEEVVGLIEWPVVLMGNFDKSFLNIPPEIIRLTIRANQKCFVTRKQGEKIKLSHHFILVSNILASDEGKE
ISKGNSKVVCARLSDALYFWQTDQHDLPDIKYLQSSAKKLDLDLKKPLDQRMAQLDHLNVTFHAKLGTQGARVERITILA
QQIAPLVHADPFLAKRAAVLAKADLQTEIVGEFPELQGLMGRKYALLQGEDPRVAEAIEDHYKPQGPADRIPQEPLAIAV
ALADKIDMLVGFWLINEKPSSSKDPYALRRAVLGVIRLVLLRDWKINLMPLFNLTANLFLQQKTPYGTSRREATQSQNVL
PEKTEHILSDLLSFFHERLKIYLKEEGARYDVLEAVLKKDADDLLLIARHVEALIAFINTSDGDSFLAAVKRTVNILEAE
SQKDSTIEDEINPELFIETEEKQLYQAITEIEKTVHHSINATNLPHLLNTLTPLGKFIDTFFEKVLVNDKNPHVRTNRLA
LLKRIHTLTQKAADFSKLVV
>Mature_739_residues
SDLLLELFSEEIPARMQRKAAADLKKSVTDQLVNAGLTYKAAHEYWTPRRLTLNIRGLSIRSKDTHEERKGPSTKSPQHV
IDGFLRATGLSDISKAHIAHDHKKGDFYIAKIVKKGRSAEEIIADILPDIIRNFPWPKSMRWGKDSEKSGALKWIRSLQN
ILCVFGPEIGETHVIPFTIGSLKSNNLTYGHRFLSDGKPLQVRRFDDYVTQLEIHKVILDAERRKNIILADAQNLCFANG
LELVKDNALLEEVVGLIEWPVVLMGNFDKSFLNIPPEIIRLTIRANQKCFVTRKQGEKIKLSHHFILVSNILASDEGKEI
SKGNSKVVCARLSDALYFWQTDQHDLPDIKYLQSSAKKLDLDLKKPLDQRMAQLDHLNVTFHAKLGTQGARVERITILAQ
QIAPLVHADPFLAKRAAVLAKADLQTEIVGEFPELQGLMGRKYALLQGEDPRVAEAIEDHYKPQGPADRIPQEPLAIAVA
LADKIDMLVGFWLINEKPSSSKDPYALRRAVLGVIRLVLLRDWKINLMPLFNLTANLFLQQKTPYGTSRREATQSQNVLP
EKTEHILSDLLSFFHERLKIYLKEEGARYDVLEAVLKKDADDLLLIARHVEALIAFINTSDGDSFLAAVKRTVNILEAES
QKDSTIEDEINPELFIETEEKQLYQAITEIEKTVHHSINATNLPHLLNTLTPLGKFIDTFFEKVLVNDKNPHVRTNRLAL
LKRIHTLTQKAADFSKLVV

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=745, Percent_Identity=30.8724832214765, Blast_Score=316, Evalue=4e-87,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 83767; Mature: 83636

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLLELFSEEIPARMQRKAAADLKKSVTDQLVNAGLTYKAAHEYWTPRRLTLNIRGLS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEEEEEEE
IRSKDTHEERKGPSTKSPQHVIDGFLRATGLSDISKAHIAHDHKKGDFYIAKIVKKGRSA
ECCCCCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCH
EEIIADILPDIIRNFPWPKSMRWGKDSEKSGALKWIRSLQNILCVFGPEIGETHVIPFTI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
GSLKSNNLTYGHRFLSDGKPLQVRRFDDYVTQLEIHKVILDAERRKNIILADAQNLCFAN
CCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHC
GLELVKDNALLEEVVGLIEWPVVLMGNFDKSFLNIPPEIIRLTIRANQKCFVTRKQGEKI
CHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHCCCHHHEEEEEECCCEEEEEECCCCEE
KLSHHFILVSNILASDEGKEISKGNSKVVCARLSDALYFWQTDQHDLPDIKYLQSSAKKL
EEEEHEEEEHHHHHCCCCCHHHCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHC
DLDLKKPLDQRMAQLDHLNVTFHAKLGTQGARVERITILAQQIAPLVHADPFLAKRAAVL
CCHHHCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AKADLQTEIVGEFPELQGLMGRKYALLQGEDPRVAEAIEDHYKPQGPADRIPQEPLAIAV
HHHCCHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHH
ALADKIDMLVGFWLINEKPSSSKDPYALRRAVLGVIRLVLLRDWKINLMPLFNLTANLFL
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEE
QQKTPYGTSRREATQSQNVLPEKTEHILSDLLSFFHERLKIYLKEEGARYDVLEAVLKKD
EECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
ADDLLLIARHVEALIAFINTSDGDSFLAAVKRTVNILEAESQKDSTIEDEINPELFIETE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEECH
EKQLYQAITEIEKTVHHSINATNLPHLLNTLTPLGKFIDTFFEKVLVNDKNPHVRTNRLA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LLKRIHTLTQKAADFSKLVV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLLLELFSEEIPARMQRKAAADLKKSVTDQLVNAGLTYKAAHEYWTPRRLTLNIRGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEEEEEEEEE
IRSKDTHEERKGPSTKSPQHVIDGFLRATGLSDISKAHIAHDHKKGDFYIAKIVKKGRSA
ECCCCCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCH
EEIIADILPDIIRNFPWPKSMRWGKDSEKSGALKWIRSLQNILCVFGPEIGETHVIPFTI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
GSLKSNNLTYGHRFLSDGKPLQVRRFDDYVTQLEIHKVILDAERRKNIILADAQNLCFAN
CCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHC
GLELVKDNALLEEVVGLIEWPVVLMGNFDKSFLNIPPEIIRLTIRANQKCFVTRKQGEKI
CHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHCCCHHHEEEEEECCCEEEEEECCCCEE
KLSHHFILVSNILASDEGKEISKGNSKVVCARLSDALYFWQTDQHDLPDIKYLQSSAKKL
EEEEHEEEEHHHHHCCCCCHHHCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHC
DLDLKKPLDQRMAQLDHLNVTFHAKLGTQGARVERITILAQQIAPLVHADPFLAKRAAVL
CCHHHCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AKADLQTEIVGEFPELQGLMGRKYALLQGEDPRVAEAIEDHYKPQGPADRIPQEPLAIAV
HHHCCHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCHHHHHH
ALADKIDMLVGFWLINEKPSSSKDPYALRRAVLGVIRLVLLRDWKINLMPLFNLTANLFL
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEE
QQKTPYGTSRREATQSQNVLPEKTEHILSDLLSFFHERLKIYLKEEGARYDVLEAVLKKD
EECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
ADDLLLIARHVEALIAFINTSDGDSFLAAVKRTVNILEAESQKDSTIEDEINPELFIETE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEECH
EKQLYQAITEIEKTVHHSINATNLPHLLNTLTPLGKFIDTFFEKVLVNDKNPHVRTNRLA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LLKRIHTLTQKAADFSKLVV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]