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The map label for this gene is rpoH2 [H]

Identifier: 49475181

GI number: 49475181

Start: 431186

End: 432070

Strand: Reverse

Name: rpoH2 [H]

Synonym: BH03810

Alternate gene names: 49475181

Gene position: 432070-431186 (Counterclockwise)

Preceding gene: 49475182

Following gene: 49475173

Centisome position: 22.37

GC content: 40.68

Gene sequence:

>885_bases
ATGGCCCATATGAATCTGCTATCAGTAACAACAGGTGATGGAGGTTTGAGTCGTTACCTGGAAGAAGTACGTCGTTTTCC
AATGCTTGAGCCGCAAGAAGAGTACATGTTGGCTCAGCGTTATCGTAAACATAACGACCTAAAAGCTGCGCATAAATTGG
TAACCAGTCACTTGCGCCTCGTAGCCAAAATCGCAGTAGGATATCGGGGCTACGGACTGCCCATTGGGGAAGTCATCTCA
GAAGGGAATGTTGGACTTATGAAGGCCGTTAAGCGTTTCGAACCAGAACGGGGTTTCCGCCTTGCAACCTATGCAATATG
GTGGATTAAAGCATCTATCCAGGAATATGTATTGCGGTCTTGGAGTTTGGTAAAAATGGGAACAACTGCTAATCAAAAGC
GCTTGTTTTTCAACCTTCGTAAGTTAAAAAGTAGACTTCAAGCACTTGACAATGGTGATCTTAATGCAGAGCAAGTAAAA
GAAATTGCAATCCAATTAAATGTCACCGAAGATGAAGTTGTCTCAATGAATCGTCGACTTGGTGGTGATACTTCACTCAA
TGCACCACTTCGAACAAAAGAAGGTGAAAATAGTGAATGGCAGGATTGGCTGGTCGATGACTCCAATAATCAGGAACAAA
CTTTAATCGAACAAAATGAATTGGAAAATCGTCGTTCCATGCTAACAAATGCCTTGGATAATTTAAATGAACGTGAAAAA
CGTATTTTTAAAGCACGTCGTTTAAGCGATATACCACTGACACTAGAAGAACTCTCAAGTGAATTCAACATCAGTCGGGA
ACGTGTAAGACAAATTGAAATGCGTGCATTTGAAAAAATACAAAATTCTATCAAAGCTTCTGCTTTATCCCAAAATCAAG
TGTAA

Upstream 100 bases:

>100_bases
AATTATGATACGATATAGGCTTGTAGAGATGTTTATTGTTTATAATGTCTAGAAATCGAATTTGCCTTATTAGGGAATTC
ATGCAAAAGGAGGGTGCTAT

Downstream 100 bases:

>100_bases
AACTTCCAAAGTTCAGTGTAGAGTCAATTATTTGTGTATTTTAAAATGGCAAAGAGCCAGCAGAAGGTTTGCTCTTTGTC
TTTGTATTAGGTTGTTGAGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAHMNLLSVTTGDGGLSRYLEEVRRFPMLEPQEEYMLAQRYRKHNDLKAAHKLVTSHLRLVAKIAVGYRGYGLPIGEVIS
EGNVGLMKAVKRFEPERGFRLATYAIWWIKASIQEYVLRSWSLVKMGTTANQKRLFFNLRKLKSRLQALDNGDLNAEQVK
EIAIQLNVTEDEVVSMNRRLGGDTSLNAPLRTKEGENSEWQDWLVDDSNNQEQTLIEQNELENRRSMLTNALDNLNEREK
RIFKARRLSDIPLTLEELSSEFNISRERVRQIEMRAFEKIQNSIKASALSQNQV

Sequences:

>Translated_294_residues
MAHMNLLSVTTGDGGLSRYLEEVRRFPMLEPQEEYMLAQRYRKHNDLKAAHKLVTSHLRLVAKIAVGYRGYGLPIGEVIS
EGNVGLMKAVKRFEPERGFRLATYAIWWIKASIQEYVLRSWSLVKMGTTANQKRLFFNLRKLKSRLQALDNGDLNAEQVK
EIAIQLNVTEDEVVSMNRRLGGDTSLNAPLRTKEGENSEWQDWLVDDSNNQEQTLIEQNELENRRSMLTNALDNLNEREK
RIFKARRLSDIPLTLEELSSEFNISRERVRQIEMRAFEKIQNSIKASALSQNQV
>Mature_293_residues
AHMNLLSVTTGDGGLSRYLEEVRRFPMLEPQEEYMLAQRYRKHNDLKAAHKLVTSHLRLVAKIAVGYRGYGLPIGEVISE
GNVGLMKAVKRFEPERGFRLATYAIWWIKASIQEYVLRSWSLVKMGTTANQKRLFFNLRKLKSRLQALDNGDLNAEQVKE
IAIQLNVTEDEVVSMNRRLGGDTSLNAPLRTKEGENSEWQDWLVDDSNNQEQTLIEQNELENRRSMLTNALDNLNEREKR
IFKARRLSDIPLTLEELSSEFNISRERVRQIEMRAFEKIQNSIKASALSQNQV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=42.1602787456446, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=31.4079422382672, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789448, Length=246, Percent_Identity=28.4552845528455, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33994; Mature: 33863

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHMNLLSVTTGDGGLSRYLEEVRRFPMLEPQEEYMLAQRYRKHNDLKAAHKLVTSHLRL
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIAVGYRGYGLPIGEVISEGNVGLMKAVKRFEPERGFRLATYAIWWIKASIQEYVLRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRKLKSRLQALDNGDLNAEQVKEIAIQLNVTEDEVVSMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
GGDTSLNAPLRTKEGENSEWQDWLVDDSNNQEQTLIEQNELENRRSMLTNALDNLNEREK
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIFKARRLSDIPLTLEELSSEFNISRERVRQIEMRAFEKIQNSIKASALSQNQV
HHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHMNLLSVTTGDGGLSRYLEEVRRFPMLEPQEEYMLAQRYRKHNDLKAAHKLVTSHLRL
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIAVGYRGYGLPIGEVISEGNVGLMKAVKRFEPERGFRLATYAIWWIKASIQEYVLRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRKLKSRLQALDNGDLNAEQVKEIAIQLNVTEDEVVSMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
GGDTSLNAPLRTKEGENSEWQDWLVDDSNNQEQTLIEQNELENRRSMLTNALDNLNEREK
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIFKARRLSDIPLTLEELSSEFNISRERVRQIEMRAFEKIQNSIKASALSQNQV
HHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]