Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 49475038

GI number: 49475038

Start: 302401

End: 303777

Strand: Reverse

Name: miaB

Synonym: BH02260

Alternate gene names: 49475038

Gene position: 303777-302401 (Counterclockwise)

Preceding gene: 49475039

Following gene: 49475037

Centisome position: 15.73

GC content: 41.1

Gene sequence:

>1377_bases
ATGAATAAAGCAAATCCTAAAAATATTCCTCCTTTGGCTCCTAAAAAGGTTTTTATCAAAACCTATGGATGCCAAATGAA
TGTTTATGACAGCCAACGGATGACTGATAGTCTTAGTTCAAAGGGTTATGTCGCTACACAAACACCAAACGACGCTGACC
TTATTCTTGTTAATACCTGTCATATCCGTGAAAAAGCAGCAGAAAAACTCTATTCAGATCTTGGTCGCTTGCGTGTCATG
CGTCAAGAACGTACACCTGACAAACCCTTGATGGTTGGTGTGACTGGTTGTGTTGCACAAGCAGAGGGAAGTGAAATTTT
GCGCCGTGCACCAATAGTGGATCTTGTTATCGGACCGCAAATGTATCACCGTTTGCCAGACTTGTTAGAACAAACCAAAC
AGGGCAAAAAAATTGTTGCAACAGAGTATGCTGTTGAGGACAAATTTGCTCATTTACCACCTCATAATAAACGTGCTGTA
AGAAAACGAGGCGTTAGTGCATTTTTAACAGTACAGGAAGGTTGTGATAAATTTTGTACTTTCTGTGTCGTTCCCTACAC
ACGTGGTGCCGAAATATCCCGATCTGTTGAGCAAATTACCGAGGAAGCCCGTCAACTCATTGAAGCTGGTGTTAAGGAAA
TTACCCTGCTTGGACAAAATGTGAATGGTTGGCATGGACAAAATGTGAATGGTAAAACTTGGCGGCTTGGTGATCTTCTT
TATCATCTTGCCAAACTAGATGGTTTAAAGCGTTTGCGTTATACGACCAGCCACCCACGAGATATGGATGACAGTCTTAT
TGCTGCACACCGAGATCTTGACATCTTGATGCCTTACCTGCATTTGCCTGTTCAATCGGGTTCAGATCGTATCTTAAAAG
CGATGAACCGCCAACACAAAAGTATTCATTATCTTCAGCTTATCGAAAAAATTCGCAATGCACGACCCGATATTGCTTTT
TCAGGCGATTTTATTGTAGGATTTCCAGGAGAGACAGATGAAGACTTTGAAGAAACCATTAAACTTATTAAACAAGTGGA
ATACAGTTCAGCCTACTCCTTTAAATATTCACCTCGACCTGGAACAGTTGGTGCAACAATGAAGAATCATGTTGAGGAAG
CTGTAAAAGATGCCAGGCTCCAGCACTTACAAGTTCTTCTCTTGGAGCAACAAAATGCATTTTTGCGTTCTAAAATTGGA
CAAACAACAGATGTTCTTATCGAAAAAGCTGGTCGTCACTCGGGACAAATGGTAGGTCGTTCACCTTGGCTTTTACCTGT
TGTCGTGGATACACAAGCCTCTACAGGCACCGTAATCCCAATTCATATTAAAAATACAAGCTCAAACAGTTTTGTCGGTG
AAATGACAAATATGTAA

Upstream 100 bases:

>100_bases
TACTACCAATCAAAAAATAGCCGCACAGATGCAATCATTATGCAAAAAACTTTTAAGCAAATAGATTGAGATTTGCGTAA
AAAACATTTACAAATGCGCT

Downstream 100 bases:

>100_bases
GCATATCTCTCATGCCTTATTTACAACAGAGATTTTAGGAGAACCCTTTTATTTTTTGAAGAAAATGATTACATTTAAAG
CTCGCACTCTATGAAAAAGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNKANPKNIPPLAPKKVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTCHIREKAAEKLYSDLGRLRVM
RQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQMYHRLPDLLEQTKQGKKIVATEYAVEDKFAHLPPHNKRAV
RKRGVSAFLTVQEGCDKFCTFCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLL
YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHKSIHYLQLIEKIRNARPDIAF
SGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRPGTVGATMKNHVEEAVKDARLQHLQVLLLEQQNAFLRSKIG
QTTDVLIEKAGRHSGQMVGRSPWLLPVVVDTQASTGTVIPIHIKNTSSNSFVGEMTNM

Sequences:

>Translated_458_residues
MNKANPKNIPPLAPKKVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTCHIREKAAEKLYSDLGRLRVM
RQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQMYHRLPDLLEQTKQGKKIVATEYAVEDKFAHLPPHNKRAV
RKRGVSAFLTVQEGCDKFCTFCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLL
YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHKSIHYLQLIEKIRNARPDIAF
SGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRPGTVGATMKNHVEEAVKDARLQHLQVLLLEQQNAFLRSKIG
QTTDVLIEKAGRHSGQMVGRSPWLLPVVVDTQASTGTVIPIHIKNTSSNSFVGEMTNM
>Mature_458_residues
MNKANPKNIPPLAPKKVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTCHIREKAAEKLYSDLGRLRVM
RQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQMYHRLPDLLEQTKQGKKIVATEYAVEDKFAHLPPHNKRAV
RKRGVSAFLTVQEGCDKFCTFCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLL
YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHKSIHYLQLIEKIRNARPDIAF
SGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRPGTVGATMKNHVEEAVKDARLQHLQVLLLEQQNAFLRSKIG
QTTDVLIEKAGRHSGQMVGRSPWLLPVVVDTQASTGTVIPIHIKNTSSNSFVGEMTNM

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=34.0336134453782, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=34.0336134453782, Blast_Score=285, Evalue=7e-77,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=25.9423503325942, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=45.3932584269663, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=432, Percent_Identity=29.8611111111111, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=444, Percent_Identity=31.5315315315315, Blast_Score=225, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI71996771, Length=336, Percent_Identity=29.1666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356207, Length=467, Percent_Identity=33.4047109207709, Blast_Score=264, Evalue=8e-71,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=27.1929824561404, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BARHE (Q6G4V6)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033079.1
- ProteinModelPortal:   Q6G4V6
- SMR:   Q6G4V6
- GeneID:   2864625
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH02260
- NMPDR:   fig|283166.1.peg.207
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q6G4V6
- ProtClustDB:   PRK14325
- BioCyc:   BHEN283166:BH02260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51439; Mature: 51439

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKANPKNIPPLAPKKVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTC
CCCCCCCCCCCCCCCEEEEEEECCEEEECCCHHHHHHHCCCCEEEECCCCCCCEEEEEEH
HIREKAAEKLYSDLGRLRVMRQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCHHHHHHHCCEEEEEECHH
MYHRLPDLLEQTKQGKKIVATEYAVEDKFAHLPPHNKRAVRKRGVSAFLTVQEGCDKFCT
HHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHH
FCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCCCCCEEEHHHHH
YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SIHYLQLIEKIRNARPDIAFSGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRP
HHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
GTVGATMKNHVEEAVKDARLQHLQVLLLEQQNAFLRSKIGQTTDVLIEKAGRHSGQMVGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCC
SPWLLPVVVDTQASTGTVIPIHIKNTSSNSFVGEMTNM
CCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHCCC
>Mature Secondary Structure
MNKANPKNIPPLAPKKVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTC
CCCCCCCCCCCCCCCEEEEEEECCEEEECCCHHHHHHHCCCCEEEECCCCCCCEEEEEEH
HIREKAAEKLYSDLGRLRVMRQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCHHHHHHHCCEEEEEECHH
MYHRLPDLLEQTKQGKKIVATEYAVEDKFAHLPPHNKRAVRKRGVSAFLTVQEGCDKFCT
HHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHCCCCEEEEHHHHHHHHHH
FCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCCCCCEEEHHHHH
YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHK
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SIHYLQLIEKIRNARPDIAFSGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRP
HHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCC
GTVGATMKNHVEEAVKDARLQHLQVLLLEQQNAFLRSKIGQTTDVLIEKAGRHSGQMVGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCCCCCC
SPWLLPVVVDTQASTGTVIPIHIKNTSSNSFVGEMTNM
CCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA