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The map label for this gene is ppa [H]

Identifier: 49474982

GI number: 49474982

Start: 229952

End: 230482

Strand: Reverse

Name: ppa [H]

Synonym: BH01690

Alternate gene names: 49474982

Gene position: 230482-229952 (Counterclockwise)

Preceding gene: 49474983

Following gene: 49474961

Centisome position: 11.94

GC content: 35.97

Gene sequence:

>531_bases
ATGAATATTAAAGAGATAGCCGTTGGTAAAAATCCACCAGAAGATGTCAATGTCATCGTTGAAGTCTCTCTTGGTGGACA
ACCAATAAAATATGAAATGGACAAAAAATCGGGCGCATTATTCGTTGATCGTTTTCTTTATACCTCGATGGTTTATCCAG
GAAATTACGGTTTTGTTCCACATACTCTTTCAGAAGATGGTGATCCCATTGATGTTCTGATCTGTAATACACGTCCACTT
CTTCCTGGCTGTGTTATTAATGTTTACCCGATCGGTGCTCTGATTATGGAAGATGACGGTGGTAAAGATGAAAAAATTAT
TGCCATCCCTACTCCCAAATTAACACAACGATATAATAATATTCATGACTATACAGATCTTCCTGAAATTACACTTAAGC
AAATTGAGCATTTCTTTGAACATTATAAAGATCTGGAACCTGGAAAATGGGCAAAAATTGAAGGTTGGCGTGATAAAAGT
TTTGCACATGAATTGATTAAGCAAGCGATTGAGCGTAATAAAGAATTATAG

Upstream 100 bases:

>100_bases
TTTTGAAAAAACACGCTCATCACTTTCAAACTAATTTCAGAACTTCATTGATCAAATGTTTTATTGTATCTAGTTTAATT
ATTCCGGAAAGGATATTTAC

Downstream 100 bases:

>100_bases
CTTTTTCAGAAAAATCTAAGCCTGTTTTTGAGCAGGCTTTCATCTTTTCTTTAATATGATCAACCCATCACGCAAAATAG
AATGTCATTGTCTTGCACAT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNIKEIAVGKNPPEDVNVIVEVSLGGQPIKYEMDKKSGALFVDRFLYTSMVYPGNYGFVPHTLSEDGDPIDVLICNTRPL
LPGCVINVYPIGALIMEDDGGKDEKIIAIPTPKLTQRYNNIHDYTDLPEITLKQIEHFFEHYKDLEPGKWAKIEGWRDKS
FAHELIKQAIERNKEL

Sequences:

>Translated_176_residues
MNIKEIAVGKNPPEDVNVIVEVSLGGQPIKYEMDKKSGALFVDRFLYTSMVYPGNYGFVPHTLSEDGDPIDVLICNTRPL
LPGCVINVYPIGALIMEDDGGKDEKIIAIPTPKLTQRYNNIHDYTDLPEITLKQIEHFFEHYKDLEPGKWAKIEGWRDKS
FAHELIKQAIERNKEL
>Mature_176_residues
MNIKEIAVGKNPPEDVNVIVEVSLGGQPIKYEMDKKSGALFVDRFLYTSMVYPGNYGFVPHTLSEDGDPIDVLICNTRPL
LPGCVINVYPIGALIMEDDGGKDEKIIAIPTPKLTQRYNNIHDYTDLPEITLKQIEHFFEHYKDLEPGKWAKIEGWRDKS
FAHELIKQAIERNKEL

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=174, Percent_Identity=52.2988505747126, Blast_Score=198, Evalue=2e-52,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 20028; Mature: 20028

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKEIAVGKNPPEDVNVIVEVSLGGQPIKYEMDKKSGALFVDRFLYTSMVYPGNYGFVP
CCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
HTLSEDGDPIDVLICNTRPLLPGCVINVYPIGALIMEDDGGKDEKIIAIPTPKLTQRYNN
CCCCCCCCEEEEEEECCCCCCCCCEEEEEECEEEEEECCCCCCCEEEEECCCHHHHHHCC
IHDYTDLPEITLKQIEHFFEHYKDLEPGKWAKIEGWRDKSFAHELIKQAIERNKEL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIKEIAVGKNPPEDVNVIVEVSLGGQPIKYEMDKKSGALFVDRFLYTSMVYPGNYGFVP
CCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
HTLSEDGDPIDVLICNTRPLLPGCVINVYPIGALIMEDDGGKDEKIIAIPTPKLTQRYNN
CCCCCCCCEEEEEEECCCCCCCCCEEEEEECEEEEEECCCCCCCEEEEECCCHHHHHHCC
IHDYTDLPEITLKQIEHFFEHYKDLEPGKWAKIEGWRDKSFAHELIKQAIERNKEL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9304784 [H]