Click here to switch to the map view.

The map label for this gene is aroA

Identifier: 49474917

GI number: 49474917

Start: 129560

End: 130888

Strand: Reverse

Name: aroA

Synonym: BH00950

Alternate gene names: 49474917

Gene position: 130888-129560 (Counterclockwise)

Preceding gene: 49474920

Following gene: 49474916

Centisome position: 6.78

GC content: 41.61

Gene sequence:

>1329_bases
ATGCAAAAAACAATACCCATGACCGCTTACAAATCTACTCGTCTTTCTGGTATAATTAAAATACCAGGAGATAAATCAAT
CTCCCATCGATCCCTTATATTGGGAGGATTAGCAAGTGGTGAAACACATATCCATGGAATACTTGAAAGCGACGATGTTT
TCAATACAGCTGCTGCCATGCAAGCTCTGGGCGCCTGTATTATTAAAAAAGATGATCTCTGGATTATTCGAGGAACCGGA
AATGGCTGCTTGTTAGCCGCACAAAAACCTTTAGATTTCGGAAATGCGGGAACAGGTGCACGCCTGGTTATGGGAATGGT
TGGACCTTACCATATGAAAACGACCTTTATTGGTGATGCCTCTCTTTCCAAACGCCCAATGGCACGTATTCTTGATCCAC
TGCAATTAATGGGTGTGGAAATTGAAGCAACCCATGGCAACTATCTCCCTTTAACACTTTATGGTCCGAAAATGACTAAC
CCAATTTGCTACCGTATTCCTGTGGCTTCTGCTCAAGTTAAATCAGCAATCTTACTTGCTGGTCTCAATACCGCCGGTAC
TACAACTGTTATCGAACCGATTCTCACACGAGATCACACGGAAAAAATGTTAAAAGCGTTCGGTGCTAAACTTGAAATAG
AAAAAAATGCAGAAGGAACGCGTTTTATTCATCTAAATGGTCACCCACACCTGACTGGACAAACAATTCATATTCCAGGT
GATCCCTCTTCTGCAGCTTTTCCTATTGTCGCAGCTCTTCTCATAGAAGACTCTGATATCACTATTGAAAATGTTCTTAT
TAACAACTCTCGAATGGGGCTTATCGAAACATTATGGGAAATGGGCGCTCAAATTGAGCTTTTGAACCAACGCCAAACAG
GTGGAGAAGATGTTGCCAACCTACGGGTTAAATCATCAGTACTAAAAGGTGTAACTGTACCCAAAGAACGTGCCCCATCA
ATGATTGATGAATATCCCGCTTTGGCAGTAGCAGCAGCCTTTGCTGAAGGCAAAACAGTTATGCTAGGAATTGAAGAATT
GCGCGTTAAAGAATCAGATCGATTGTCTGTACTTGCTCAAGGACTTAAAATTAACCACGTAGACTGTGAAGAAGGTCAAG
ATTTTCTCATTGTTCATGGAAAAGGCTCCGCCAAAGGATTGGGTGGAGGACATGTCACTACACATCTTGATCATCGAATT
GCCATGTCCTTTCTTATCTTTGGACTGGTCTCAGAAAAACCTGTTACCATTGATGATAAACGAATGATTGCTACCAGCTT
TCCAGAATTTATCCCTTTTATACAACAACTTGGGGGAAAAATCTCTTGA

Upstream 100 bases:

>100_bases
AAATACATCGTTTCATATGGATTTCCTCATGACGCCTTTCAAAGCATGTGCTAAAGAGTACTTTATTTAATAAAAAACAG
GAGAATTAAATAGAACTTCT

Downstream 100 bases:

>100_bases
AACCTTTCGTGATTGCAATTGATGGACCAGCAGCTTCAGGAAAAGGGACGTTAGCACGAAAAATTGCTACGCACTACCAT
CTTCACTATCTCGATACTGG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MQKTIPMTAYKSTRLSGIIKIPGDKSISHRSLILGGLASGETHIHGILESDDVFNTAAAMQALGACIIKKDDLWIIRGTG
NGCLLAAQKPLDFGNAGTGARLVMGMVGPYHMKTTFIGDASLSKRPMARILDPLQLMGVEIEATHGNYLPLTLYGPKMTN
PICYRIPVASAQVKSAILLAGLNTAGTTTVIEPILTRDHTEKMLKAFGAKLEIEKNAEGTRFIHLNGHPHLTGQTIHIPG
DPSSAAFPIVAALLIEDSDITIENVLINNSRMGLIETLWEMGAQIELLNQRQTGGEDVANLRVKSSVLKGVTVPKERAPS
MIDEYPALAVAAAFAEGKTVMLGIEELRVKESDRLSVLAQGLKINHVDCEEGQDFLIVHGKGSAKGLGGGHVTTHLDHRI
AMSFLIFGLVSEKPVTIDDKRMIATSFPEFIPFIQQLGGKIS

Sequences:

>Translated_442_residues
MQKTIPMTAYKSTRLSGIIKIPGDKSISHRSLILGGLASGETHIHGILESDDVFNTAAAMQALGACIIKKDDLWIIRGTG
NGCLLAAQKPLDFGNAGTGARLVMGMVGPYHMKTTFIGDASLSKRPMARILDPLQLMGVEIEATHGNYLPLTLYGPKMTN
PICYRIPVASAQVKSAILLAGLNTAGTTTVIEPILTRDHTEKMLKAFGAKLEIEKNAEGTRFIHLNGHPHLTGQTIHIPG
DPSSAAFPIVAALLIEDSDITIENVLINNSRMGLIETLWEMGAQIELLNQRQTGGEDVANLRVKSSVLKGVTVPKERAPS
MIDEYPALAVAAAFAEGKTVMLGIEELRVKESDRLSVLAQGLKINHVDCEEGQDFLIVHGKGSAKGLGGGHVTTHLDHRI
AMSFLIFGLVSEKPVTIDDKRMIATSFPEFIPFIQQLGGKIS
>Mature_442_residues
MQKTIPMTAYKSTRLSGIIKIPGDKSISHRSLILGGLASGETHIHGILESDDVFNTAAAMQALGACIIKKDDLWIIRGTG
NGCLLAAQKPLDFGNAGTGARLVMGMVGPYHMKTTFIGDASLSKRPMARILDPLQLMGVEIEATHGNYLPLTLYGPKMTN
PICYRIPVASAQVKSAILLAGLNTAGTTTVIEPILTRDHTEKMLKAFGAKLEIEKNAEGTRFIHLNGHPHLTGQTIHIPG
DPSSAAFPIVAALLIEDSDITIENVLINNSRMGLIETLWEMGAQIELLNQRQTGGEDVANLRVKSSVLKGVTVPKERAPS
MIDEYPALAVAAAFAEGKTVMLGIEELRVKESDRLSVLAQGLKINHVDCEEGQDFLIVHGKGSAKGLGGGHVTTHLDHRI
AMSFLIFGLVSEKPVTIDDKRMIATSFPEFIPFIQQLGGKIS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=427, Percent_Identity=29.5081967213115, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320332, Length=469, Percent_Identity=28.7846481876333, Blast_Score=119, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_BARHE (Q6G545)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_032958.1
- HSSP:   Q9S400
- ProteinModelPortal:   Q6G545
- SMR:   Q6G545
- GeneID:   2866077
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH00950
- NMPDR:   fig|283166.1.peg.86
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   BHEN283166:BH00950-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 47516; Mature: 47516

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTIPMTAYKSTRLSGIIKIPGDKSISHRSLILGGLASGETHIHGILESDDVFNTAAAM
CCCCCCCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
QALGACIIKKDDLWIIRGTGNGCLLAAQKPLDFGNAGTGARLVMGMVGPYHMKTTFIGDA
HHHCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCC
SLSKRPMARILDPLQLMGVEIEATHGNYLPLTLYGPKMTNPICYRIPVASAQVKSAILLA
CCCCCCHHHHCCHHHHHCEEEEECCCCEEEEEEECCCCCCCEEEEEECCHHHHCCEEEEE
GLNTAGTTTVIEPILTRDHTEKMLKAFGAKLEIEKNAEGTRFIHLNGHPHLTGQTIHIPG
ECCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCC
DPSSAAFPIVAALLIEDSDITIENVLINNSRMGLIETLWEMGAQIELLNQRQTGGEDVAN
CCCCCHHHHEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRVKSSVLKGVTVPKERAPSMIDEYPALAVAAAFAEGKTVMLGIEELRVKESDRLSVLAQ
HHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHCCCCEEEEEHHHHHCCCCCHHHHHHC
GLKINHVDCEEGQDFLIVHGKGSAKGLGGGHVTTHLDHRIAMSFLIFGLVSEKPVTIDDK
CCEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCC
RMIATSFPEFIPFIQQLGGKIS
EEEHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQKTIPMTAYKSTRLSGIIKIPGDKSISHRSLILGGLASGETHIHGILESDDVFNTAAAM
CCCCCCCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHH
QALGACIIKKDDLWIIRGTGNGCLLAAQKPLDFGNAGTGARLVMGMVGPYHMKTTFIGDA
HHHCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCC
SLSKRPMARILDPLQLMGVEIEATHGNYLPLTLYGPKMTNPICYRIPVASAQVKSAILLA
CCCCCCHHHHCCHHHHHCEEEEECCCCEEEEEEECCCCCCCEEEEEECCHHHHCCEEEEE
GLNTAGTTTVIEPILTRDHTEKMLKAFGAKLEIEKNAEGTRFIHLNGHPHLTGQTIHIPG
ECCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCEEECCC
DPSSAAFPIVAALLIEDSDITIENVLINNSRMGLIETLWEMGAQIELLNQRQTGGEDVAN
CCCCCHHHHEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHH
LRVKSSVLKGVTVPKERAPSMIDEYPALAVAAAFAEGKTVMLGIEELRVKESDRLSVLAQ
HHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHCCCCEEEEEHHHHHCCCCCHHHHHHC
GLKINHVDCEEGQDFLIVHGKGSAKGLGGGHVTTHLDHRIAMSFLIFGLVSEKPVTIDDK
CCEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCC
RMIATSFPEFIPFIQQLGGKIS
EEEHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA