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The map label for this gene is pyrP [H]

Identifier: 49186740

GI number: 49186740

Start: 3709824

End: 3711140

Strand: Reverse

Name: pyrP [H]

Synonym: BAS3741

Alternate gene names: 49186740

Gene position: 3711140-3709824 (Counterclockwise)

Preceding gene: 49186741

Following gene: 49186739

Centisome position: 70.98

GC content: 39.79

Gene sequence:

>1317_bases
TTGCAAAGATTTAAACTGAAGGAGGATGTAACAATGGAACAAAAGCCAGTGTTAGACGTTAATGAAGTACCGAAACCGGG
AAAATGGTTATTTTTAAGTATACAACATTTGTTCGCGATGTTTGGATCAACAGTGCTTGTTCCGTTTTTAACAGGATTGA
ATCCGTCAGTAGCATTAATATCAAGTGGATTAGGAACGTTAGCGTTTCTTCTTATAACGAAAGGTCAAGTACCTGCCTAT
CTTGGATCATCATTCGCCTTTATCGCACCGATTATAACAGCGAAAACGGCAGGGGGACCCGGAGCAGCAATGCTTGGTGG
TTTGCTAGCAGGACTTGTATACATCTTAATCTCACTCGGAATTAAGAAATCAGGATCAAAGTGGATTATGAAATTACTAC
CACCAATCGTAGTTGGTCCAGTAGTAATGGTAATCGGGCTAGCTTTAGCACATACAGCAGTTAACATGGCGATGAATGGT
GCGGATGGCAAATATAGCTTTACACACTTTTCAGTAGCATTAGTAACATTAGCAATTACAATCATTTGCTCCATATTCGG
GAGAGGGTTCTTCAGTATCATACCAGTTTTACTTGGAATCATCGGAGGTTACATCTTCGCTTACTTCCAAGGGCTAGTAG
ACTTAAAGCCGGTAGCTGAGGCAAAATGGTTTGTTGTACCAGATTTCACAGTACCATTTGTAACATACACACCAGAGTTT
TCATGGAAGATTGTACTCTTGATGGTACCAGTTGCACTAGTAACAATTTCAGAACATATCGGACATCAAATTGTATTAGG
GAATGTTATTAAAAGAGATTTAATTGAAAAACCAGGTTTACACCGTTCGATCTTCGGTGATGGAGTAGCAACATTAATCG
CATCACTAATCGGTGGACCACCGAATACAACGTATGGTGAAAACATCGGCGTGCTCGCAATTACAAGAGCGTACAGTGTA
TACTTATTCATCGGTTCAGCGGTATTCGCAATCATGTTCGGATTTATTGGTAAGATTTCTGCACTGATTCATTCGATCCC
AACACCAGTTATGGGTGGTGTATCAATCTTACTATTCGGTGTAATCGCATCAAGCGGATTACGCATGATGGTAGACGATA
AAACAGATTTAAGTGACAAACGAAACTTAATGATTGCATCAGTAATACTAGTAATCGGTATTGGTGGAGCGGTACTTCAC
GTAGGAGAATCATTCCAAGTAGAAGGAATGGCACTAGCAGCAATTGTAGGTGTACTGTTAAATCTACTACTACCGGAAAC
GAAACAAATAAAACAATCTAAGCAGATTGCTTCATAA

Upstream 100 bases:

>100_bases
GTAGTCCTGTGAGGCTGCCAAAGGGTCTTGGCTTTCATATGTCAAATTTGGCATACGAAAGTGTAGGACTCTTTGTGCAC
AGGCACAAAGAGTTTTTTTA

Downstream 100 bases:

>100_bases
TAATAACCTTTTAATTCAGTCCTGTGAGACCGGAAAGGGTGGCTTCTTTCTTGCACCCTAGTCTCACATGACTAGGGTAT
TTTTATGCGAAGGTTGTTAT

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQRFKLKEDVTMEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAY
LGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNG
ADGKYSFTHFSVALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEF
SWKIVLLMVPVALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSV
YLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLH
VGESFQVEGMALAAIVGVLLNLLLPETKQIKQSKQIAS

Sequences:

>Translated_438_residues
MQRFKLKEDVTMEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAY
LGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNG
ADGKYSFTHFSVALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEF
SWKIVLLMVPVALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSV
YLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLH
VGESFQVEGMALAAIVGVLLNLLLPETKQIKQSKQIAS
>Mature_438_residues
MQRFKLKEDVTMEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALISSGLGTLAFLLITKGQVPAY
LGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLGIKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNG
ADGKYSFTHFSVALVTLAITIICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEF
SWKIVLLMVPVALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGPPNTTYGENIGVLAITRAYSV
YLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFGVIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLH
VGESFQVEGMALAAIVGVLLNLLLPETKQIKQSKQIAS

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=459, Percent_Identity=24.1830065359477, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI40316845, Length=459, Percent_Identity=24.1830065359477, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI44680145, Length=445, Percent_Identity=24.0449438202247, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI44680143, Length=446, Percent_Identity=23.9910313901345, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1788843, Length=412, Percent_Identity=45.6310679611651, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI87081818, Length=437, Percent_Identity=37.9862700228833, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082181, Length=440, Percent_Identity=25.9090909090909, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790087, Length=457, Percent_Identity=24.507658643326, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082178, Length=316, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=464, Percent_Identity=23.4913793103448, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=429, Percent_Identity=24.4755244755245, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21356175, Length=448, Percent_Identity=25.4464285714286, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 46463; Mature: 46463

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFKLKEDVTMEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SSGLGTLAFLLITKGQVPAYLGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLG
HCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
IKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFSVALVTLAIT
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
IICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEF
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCC
SWKIVLLMVPVALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGP
CHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCC
PNTTYGENIGVLAITRAYSVYLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFG
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMALAAIVGVLL
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHH
NLLLPETKQIKQSKQIAS
HHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQRFKLKEDVTMEQKPVLDVNEVPKPGKWLFLSIQHLFAMFGSTVLVPFLTGLNPSVALI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SSGLGTLAFLLITKGQVPAYLGSSFAFIAPIITAKTAGGPGAAMLGGLLAGLVYILISLG
HCCHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
IKKSGSKWIMKLLPPIVVGPVVMVIGLALAHTAVNMAMNGADGKYSFTHFSVALVTLAIT
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
IICSIFGRGFFSIIPVLLGIIGGYIFAYFQGLVDLKPVAEAKWFVVPDFTVPFVTYTPEF
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCC
SWKIVLLMVPVALVTISEHIGHQIVLGNVIKRDLIEKPGLHRSIFGDGVATLIASLIGGP
CHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCC
PNTTYGENIGVLAITRAYSVYLFIGSAVFAIMFGFIGKISALIHSIPTPVMGGVSILLFG
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VIASSGLRMMVDDKTDLSDKRNLMIASVILVIGIGGAVLHVGESFQVEGMALAAIVGVLL
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHEECCCCCCCCHHHHHHHHHHHH
NLLLPETKQIKQSKQIAS
HHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]