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The map label for this gene is kinE [H]

Identifier: 49186436

GI number: 49186436

Start: 3401883

End: 3403415

Strand: Direct

Name: kinE [H]

Synonym: BAS3432

Alternate gene names: 49186436

Gene position: 3401883-3403415 (Clockwise)

Preceding gene: 49186434

Following gene: 49186448

Centisome position: 65.06

GC content: 33.66

Gene sequence:

>1533_bases
ATGAGTCGTAAAAAATATTCATCAACTCAAAAAAAACGTCATCCGAACATTTCTAGACGTTTGCGAAACCATATACAAGA
TCGTTCATTAACAGAAATGTACGCATCCCTGTTCGAACACAATCCTGATAGTATTATTTCATTAAATTTAGAAGGAGTTA
TTCTACATATAAATCCTTCTGCCGAAAAAATATTAGGTTACACTTCTTTAGAATTAGAACAGAAAAATATTACCTCTATT
TTAGAAGCCCATATTTCTGAGCAAGTATTACAAAATATAAAGAATACGGAAACGGATAATCAACAAGAGTATATTTTATC
TATTTGTCATAAAAATGGATTCTTATTAGACGTCGTGACAAAACTAGTTCCTATTTTTGTCCAAAATCGTCTTACTGGCG
TATATGCCATTATGAAGCCGCTTGAAAAATCAGAAAGAATTGAGAATATGCTAAAAGAAAGTGAGAAGAGATTACGTACA
TTAGTGAACTCTATGCCTGCGTTTGTTATATTTAAAGATCATGAAGGACGCTGGCTTGAGGCAAATGACTACGCACTTTC
TTGTTTCAATTTCCATCATGTACCTTACCGTGGGAAAAAAGATAGTGAACTCATTCAATATAATGAAGCGTACCGAGAAG
CTTTTTTACATTGCGAAGAAGTCGATGAACTAGCATGGCAACAAAGACAAATTCTTCATGGTGAGGAATTTATTATTCAT
AGAGATGATTCCGATCTAATCTTAAGTATGTCGAAAGTTCCTCTCTATCATCCTGACGGCTCCCGTAAAGGTCTTATCGT
AATGGGAAGAGACGTTACTGAGTTAAAAGAAACAGAAAAGTTATTACGAAAATCAGAAAAACTCGCAGTAGTCGGACAAC
TGACAGCTGGAATTGCCCATGAAATTCGAAATCCACTCACTTCTCTAAAAGGTTTTCTAACATTGCTACTCCCTGAAATA
AATGAGGAGCATAAATGGTATATCGATGTTATGTTAAGTGAAATTTCACAAATGGAATCTATTACAAGTCAATTTATGGC
GATGTCTAAGCCACAAGTATTATCAATCAAAACTTGTAACATACAAACTTTGATTGAAGAGGTAGTTACATTTATTTTAC
CAACTGCTATTATGCATAGTGTTCACATCATTATGGATCATTTTGATTACGCACATGATATTCAATGTGACGGTAACCAA
TTAAAACAGGTTTTTATAAATATATTAAAAAATGCGATTGAAGCGATGCCAAATGGCGGAAATATTTTCATCCAAACAAA
ACCATTAGAAGATACCTTTATTTTAATACGGATTATCGATGAAGGTTGCGGTATCCCAGAAGATCGTATCTCTCGTCTAG
GCGAACCATTTTATAGTTTAAAAGAAAAGGGAACTGGACTAGGGCTAATGATGTGTTACAAAATAATTGAAGAACACCAT
GGTAAATTACACATTTCAAGCGAATTAAATAAAGGTACGATCGTTGACATTCAATTACCACTTTCTTCATCACATCTTAC
AATTCAGTCTTAA

Upstream 100 bases:

>100_bases
ATTATATAACTTCCATTTTTTATAGAAATACACGTTACATCTATATTATAGTTATGAATAGACAAATCTTTTTAGATGCT
ATTTGTACGGAGGAACTATT

Downstream 100 bases:

>100_bases
ATAAAAAAGAAGCATCTGCTAATGATTGCAAATGCTTCTTTCTTTATTTACTTTTATAGTACTGTATTTTCATACCATTC
TTTTGCTTTTTCTACTTCAC

Product: sporulation kinase A

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAEKILGYTSLELEQKNITSI
LEAHISEQVLQNIKNTETDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYAIMKPLEKSERIENMLKESEKRLRT
LVNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
RDDSDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEI
NEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLSIKTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYAHDIQCDGNQ
LKQVFINILKNAIEAMPNGGNIFIQTKPLEDTFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
GKLHISSELNKGTIVDIQLPLSSSHLTIQS

Sequences:

>Translated_510_residues
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAEKILGYTSLELEQKNITSI
LEAHISEQVLQNIKNTETDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYAIMKPLEKSERIENMLKESEKRLRT
LVNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
RDDSDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEI
NEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLSIKTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYAHDIQCDGNQ
LKQVFINILKNAIEAMPNGGNIFIQTKPLEDTFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
GKLHISSELNKGTIVDIQLPLSSSHLTIQS
>Mature_509_residues
SRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPSAEKILGYTSLELEQKNITSIL
EAHISEQVLQNIKNTETDNQQEYILSICHKNGFLLDVVTKLVPIFVQNRLTGVYAIMKPLEKSERIENMLKESEKRLRTL
VNSMPAFVIFKDHEGRWLEANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIHR
DDSDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAHEIRNPLTSLKGFLTLLLPEIN
EEHKWYIDVMLSEISQMESITSQFMAMSKPQVLSIKTCNIQTLIEEVVTFILPTAIMHSVHIIMDHFDYAHDIQCDGNQL
KQVFINILKNAIEAMPNGGNIFIQTKPLEDTFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHHG
KLHISSELNKGTIVDIQLPLSSSHLTIQS

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=397, Percent_Identity=27.2040302267003, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=31.25, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994928, Length=372, Percent_Identity=21.7741935483871, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790300, Length=209, Percent_Identity=30.1435406698565, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786600, Length=381, Percent_Identity=23.8845144356955, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=26.1061946902655, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=27.9279279279279, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790551, Length=205, Percent_Identity=28.780487804878, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=408, Percent_Identity=21.078431372549, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=20.9876543209877, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=21.5596330275229, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58578; Mature: 58446

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPS
CCCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
AEKILGYTSLELEQKNITSILEAHISEQVLQNIKNTETDNQQEYILSICHKNGFLLDVVT
HHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
KLVPIFVQNRLTGVYAIMKPLEKSERIENMLKESEKRLRTLVNSMPAFVIFKDHEGRWLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
ANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RDDSDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAH
ECCCCCEEEECCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIRNPLTSLKGFLTLLLPEINEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLSIKTCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
IQTLIEEVVTFILPTAIMHSVHIIMDHFDYAHDIQCDGNQLKQVFINILKNAIEAMPNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NIFIQTKPLEDTFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
EEEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKLHISSELNKGTIVDIQLPLSSSHLTIQS
CEEEEECCCCCCEEEEEEECCCCCCEEEEC
>Mature Secondary Structure 
SRKKYSSTQKKRHPNISRRLRNHIQDRSLTEMYASLFEHNPDSIISLNLEGVILHINPS
CCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCC
AEKILGYTSLELEQKNITSILEAHISEQVLQNIKNTETDNQQEYILSICHKNGFLLDVVT
HHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHH
KLVPIFVQNRLTGVYAIMKPLEKSERIENMLKESEKRLRTLVNSMPAFVIFKDHEGRWLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
ANDYALSCFNFHHVPYRGKKDSELIQYNEAYREAFLHCEEVDELAWQQRQILHGEEFIIH
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
RDDSDLILSMSKVPLYHPDGSRKGLIVMGRDVTELKETEKLLRKSEKLAVVGQLTAGIAH
ECCCCCEEEECCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIRNPLTSLKGFLTLLLPEINEEHKWYIDVMLSEISQMESITSQFMAMSKPQVLSIKTCN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
IQTLIEEVVTFILPTAIMHSVHIIMDHFDYAHDIQCDGNQLKQVFINILKNAIEAMPNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NIFIQTKPLEDTFILIRIIDEGCGIPEDRISRLGEPFYSLKEKGTGLGLMMCYKIIEEHH
EEEEEECCCCCCEEEEEEECCCCCCCHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKLHISSELNKGTIVDIQLPLSSSHLTIQS
CEEEEECCCCCCEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]