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The map label for this gene is yhfT [H]

Identifier: 49186423

GI number: 49186423

Start: 3390153

End: 3391832

Strand: Reverse

Name: yhfT [H]

Synonym: BAS3419

Alternate gene names: 49186423

Gene position: 3391832-3390153 (Counterclockwise)

Preceding gene: 49186424

Following gene: 49186422

Centisome position: 64.87

GC content: 34.58

Gene sequence:

>1680_bases
GTGGGAGACGATGAAAGTGAGTGCTGTGTTTTTACCAGGAGATTGCATAAAAGCTATTATTGCAGCGATTCTTGTAACGA
AATTACATCGTTCATTGAAACATATTATTACGCCTGCTCTAAAGAATGGGAAATATACAAATGCGGGATAAGAAAGAGAA
GAAACACGTTTATTGCGTGTTTCTTTTTAGTATTTTGTAAAGAAAGGACATCTCATATGGGAATAACAAAAGAATATAAA
AAACATGCCTCTTTACAACCGAATAAAATAGCAATAAAGGAAAATGATCGAGTTTTAACATATAAAGAGTGGTTTGAGTC
AGTGTATAAAGTAGCAAAATGGTTGAATGAAAAAGAATCGAAGAATAAAACGATAGCGATTGTATTAGAAAATCGTATAG
AGTTTTTACAACTATTCGCGGGTGCTGCTATGGCCGGATGGGTTTGTGTGCCACTAGATATAAAGTGGAAACAAGATGAG
CTCAAAGAAAGAATTGCAATTAGTAATCCGGATATGATTGTGACAGAGAGATATAAGTTAAATGATTTACCGAATGAAGA
AGGAAGAGTAATTGAAATTGATGAGTGGAAAAGGATGATTGAGAAATATCTTCCTACATATTTCCCTATAGAAAATGTAC
AAAATGCTCCTTTTTATATGGGATTCACATCAGGATCGACTGGAAAAGCAAAAGCGTTTTTACGTGCGCAACAATCGTGG
CTTCATAGTTTTGATTGTAATGTACATGATTTTCATATGAAAAGAGAGGATTCGATTTTAATAGCTGGGACGCTTGTTCA
TTCTCTTTTCTTATACGGGGCAATAAGCGCATTATATGTAGGACAAACGGTTCACATTATGAGAAAGTTCATTCCAAATC
AAGTATTAGATCAGTTAGAAACTGAAAATATATCAGTTATGTATACAGTTCCGACAATGCTTGAATCTCCATATAAAGAA
AATAGAGTGATAGAAAATAAAATGAAAATTATTTCGTCAGGGGCGAAATGGGAAGCTGGAGCGAAAGAAAAAATAAAGAA
TATCTTTCCTTATGCGAAAAGATATGAATTTTATGGTGCATCGGAACTGAGTTTTGTAACAGCATTAGTTGATGAAGAGA
GCGAAAGAAGGCCAAATTCAGTAGGGAAACCTTGTCACAATGTGCAAGTTCGAGTATGTAATGAGGCAGGAAAAGAAGTA
CAGAAAGGTGAGATAGGAACTGTTTATGTGAAAAGTGATCAGTTTTTTATGGGATACATAATAGATGGTGTTTTAGCACG
GGAGTTGAATGCAGATGGCTGGATGACAGTGCGAGATGTAGGTTATGAAGATGAAGAAGGATTTATTTATATTATCGGTA
GAGAGAAGAATATGATTTTATTTGGAGGAATTAATATTTTCCCAGAAGAAATAGAAAGCGTATTACATGAACATCCAGCT
GTTGATGAAATAGTTGTAATTGGTGTGGAAGATAGTTACTGGGGTGAAAAACCTGTCGCGATTGTAAAAGGAAGTGCTAC
GAGGCAACAATTAAAGCGTTTTTGCTTACAAAGATTATCCTCTTTTAAAATACCGAAAGAATGGTATTTTGTAGATGAAA
TACCGTATACAAATAGCGGGAAAATCGCTCGTATGGAAGCAAAGAGTATCATTGAAAATCAGGAGAAAATATATGAATAG

Upstream 100 bases:

>100_bases
TTTCTATTATAAAAGTATTATGCATTAATATAATTGGTGGTATTTTCGTAGTTTATGTATTTGGTATTACTGTACAAGCT
TTCTTAATGGGTGTTTCCGT

Downstream 100 bases:

>100_bases
AGCAGTTATTGTAGAAGCGAAAAGGACACCTATTGGTAAGAAGAATGGGATGTTAAAAGACTATGAAGTTCAGCAATTAG
CAGCACCGCTTCTTACATTT

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESPYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Sequences:

>Translated_559_residues
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYK
KHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDE
LKERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESPYKE
NRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEV
QKGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE
>Mature_558_residues
GDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIACFFLVFCKERTSHMGITKEYKK
HASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKESKNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDEL
KERIAISNPDMIVTERYKLNDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSWL
HSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLETENISVMYTVPTMLESPYKEN
RVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGASELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQ
KGEIGTVYVKSDQFFMGYIIDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPAV
DEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSGKIARMEAKSIIENQEKIYE

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=356, Percent_Identity=28.0898876404494, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=516, Percent_Identity=24.8062015503876, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761343, Length=516, Percent_Identity=24.8062015503876, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI58082049, Length=471, Percent_Identity=23.3545647558386, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI157311624, Length=474, Percent_Identity=22.9957805907173, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI157311622, Length=474, Percent_Identity=22.9957805907173, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=27.4647887323944, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=375, Percent_Identity=24.2666666666667, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI38505220, Length=353, Percent_Identity=24.929178470255, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI115511026, Length=468, Percent_Identity=23.5042735042735, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI13375727, Length=427, Percent_Identity=20.6088992974239, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=24.3346007604563, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=23.8568588469185, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.9766081871345, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI221142682, Length=202, Percent_Identity=32.6732673267327, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788595, Length=336, Percent_Identity=25.8928571428571, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790505, Length=424, Percent_Identity=21.9339622641509, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=534, Percent_Identity=24.9063670411985, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=25.9259259259259, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=23.719165085389, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=301, Percent_Identity=30.5647840531561, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=365, Percent_Identity=27.9452054794521, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=28.099173553719, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=347, Percent_Identity=25.9365994236311, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=347, Percent_Identity=25.9365994236311, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.7617728531856, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=347, Percent_Identity=25.9365994236311, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=485, Percent_Identity=23.9175257731959, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=493, Percent_Identity=24.5436105476673, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=485, Percent_Identity=21.4432989690722, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=533, Percent_Identity=25.703564727955, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=24.0213523131673, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24581924, Length=341, Percent_Identity=28.4457478005865, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=24.4855967078189, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21356947, Length=246, Percent_Identity=25.609756097561, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18859661, Length=498, Percent_Identity=24.4979919678715, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=23.3400402414487, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356441, Length=505, Percent_Identity=22.5742574257426, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=22.4852071005917, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653035, Length=200, Percent_Identity=28, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648255, Length=507, Percent_Identity=22.2879684418146, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358303, Length=182, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI161076582, Length=205, Percent_Identity=27.8048780487805, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922652, Length=356, Percent_Identity=23.0337078651685, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI20130357, Length=252, Percent_Identity=24.2063492063492, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281365686, Length=226, Percent_Identity=26.1061946902655, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24656500, Length=344, Percent_Identity=25, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62472339, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667959, Length=402, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648257, Length=499, Percent_Identity=21.2424849699399, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355225, Length=388, Percent_Identity=22.680412371134, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 64861; Mature: 64730

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEEHHHHCCCCHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDDESECCVFTRRLHKSYYCSDSCNEITSFIETYYYACSKEWEIYKCGIRKRRNTFIAC
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCHHHHH
FFLVFCKERTSHMGITKEYKKHASLQPNKIAIKENDRVLTYKEWFESVYKVAKWLNEKES
HHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHCC
KNKTIAIVLENRIEFLQLFAGAAMAGWVCVPLDIKWKQDELKERIAISNPDMIVTERYKL
CCCEEEEEEHHHHHHHHHHHHHHHHCEEEEEEEEEECHHHHHHHHCCCCCCEEEEECEEC
NDLPNEEGRVIEIDEWKRMIEKYLPTYFPIENVQNAPFYMGFTSGSTGKAKAFLRAQQSW
CCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
LHSFDCNVHDFHMKREDSILIAGTLVHSLFLYGAISALYVGQTVHIMRKFIPNQVLDQLE
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TENISVMYTVPTMLESPYKENRVIENKMKIISSGAKWEAGAKEKIKNIFPYAKRYEFYGA
CCCEEEEEEEHHHHCCCCHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEECCCC
SELSFVTALVDEESERRPNSVGKPCHNVQVRVCNEAGKEVQKGEIGTVYVKSDQFFMGYI
CHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEECHHCCHHHCCCCEEEEEECCCEEEHHH
IDGVLARELNADGWMTVRDVGYEDEEGFIYIIGREKNMILFGGINIFPEEIESVLHEHPA
HHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
VDEIVVIGVEDSYWGEKPVAIVKGSATRQQLKRFCLQRLSSFKIPKEWYFVDEIPYTNSG
CCCEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
KIARMEAKSIIENQEKIYE
CEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]