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The map label for this gene is tcmG [H]

Identifier: 49186309

GI number: 49186309

Start: 3269261

End: 3270895

Strand: Reverse

Name: tcmG [H]

Synonym: BAS3304

Alternate gene names: 49186309

Gene position: 3270895-3269261 (Counterclockwise)

Preceding gene: 49186316

Following gene: 49186308

Centisome position: 62.56

GC content: 37.06

Gene sequence:

>1635_bases
GTGGAGGATAAGTATATGGAATCGAATGATGTACCAGTTTTAATTGTTGGAGGGGGATTATCAGGATTAGCGTCTGCGTT
ATTTCTTGCAAAACATAATATTGAGTACTTACTTATTGAAAGGCATCCGTCTACTGCAATTCATCCAAAAGCAGGTGGAA
TCACTTTTCGTACGATGGAGTTATTTCGAGAATTAGGTTTAGAACAAAGAATTAGATCGGCGGGTAAAACATTAGAAAAT
TGCCGAGGAAGAATAGCTGTTCATACAATAGCTGAGGCGAATCAGGAAGAATTGGCACAAATGAGGGCGAATCAGTATGA
AAATGATGAAAAGTTACTTCAGAAAATAGAAGAGATTAGTCCATCAAAACAAACTGCTTGTTATCAAATTACTTTAGAAG
AGATCATGTTACAAGAAGCGAGAATATTAGGCGGAGAATTATCTTTTTATCATGAATTAGTTTCTTATGAACAAAATGAG
CAAGGAGTAATCGCAACGATTCGAAATCGTGAAACAGAAAAAGAAAGTGTCATACATTGTGACTATGTAATTGCAGCAGA
TGGGGCAAAAAGTAAAATACGTGAGCAGCTAGGGATAAGTGCGGAGGGACGAGGTACAATCGGTGGCTATTATATGAATA
TTTATTTTGAAGCGGATGTAAGTGAGTTTATGCAAGGAGATGCATTTGGTTTTTCTATGGTACTTCATCCGGAAGTTCTC
GGTGCACTCATTCCAGTCGATAACGCAAGAAGGTGGATTTATCATGTATCCTACGATCCATTAAAAGGAGAGCGACCAGA
AGATTTCACTATAGAACGTTGTAAACAAATTATTCAAACCGCAATTGGAAGCACAAATGTTGAGCCGGAAATAGTAAGTG
TTTTACCGTGGAAAGCGGCTGAAAGTACTGCTACAAAATTTCAAGATAATCGCATTTTTTTAGTTGGTGATTCTGCACAT
ATTATGCCGCCAACAGGAGGGTTTGGTTCCAATACAGGAATACAAGATGCACACAATCTAGCCTGGAAATTAGCGGCTGT
TATAAAAGGAAAGGCAAAACCAAAACTGTTAGAAACATATCATGAAGAGAGATATCCTGTTGCAAAATTAACGACAGATT
ATGCGATTAGTTTACTGTTTCGTGCTGCGAATAGAGAGGAAGGCAGTTTGAATAAGATGGATGGTTTAGCTGTAACTGCT
GGGTATCATTATAGTTCAGATGCAATAATAGATGACGCAGTCACTCTACATAGAATGGATATAGTACAGTTGAACGGAAG
ACCTGGAACGCGAGCACCACATTTTATTGGAATGTATGAGGAGAAAGAAGTCTCTGTCCTTGATTTATTAGGTAACGATT
TTGTTTTGCTTACTGGAGCAGAAAATGATTCTTGGGCTGAATCAGCACATACTGTTTCAGCTAAATTAGGAGTAAATATA
AAAGTATATCGCGTTGGTTTAAGTGGAGATTTTATTGCTCAAGAAAATATTTTTAGCGAATTATATGGTTTAAGTGATGG
AGGAGCTGTATTAATTAGACCAGATGCTTTTATAGGATGGCGTTCGGAAAAAGAGGTAGTAAATCTAGATGTAGTGCTTG
AAGAGGTAATGAGTAATTTATTATGTGATTTTTAA

Upstream 100 bases:

>100_bases
GGGGCGTAATCTATCTATATGATAATTTAAAATGAAATATAAGTCTATATCTTTGTTGTTTTTTTGATTATATTTATATC
ACTGAGCTCAGAACAATGAT

Downstream 100 bases:

>100_bases
GTAACTAAGGAAGTATTGTCTTTTATAGGACGATACTTCTATTTTTTTAGCTTAAAAAATAAAGATTGTGTAGTGAAGAG
ACATTATTATTATAAAAATA

Product: PheA/TfdB family FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MEDKYMESNDVPVLIVGGGLSGLASALFLAKHNIEYLLIERHPSTAIHPKAGGITFRTMELFRELGLEQRIRSAGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARILGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADVSEFMQGDAFGFSMVLHPEVL
GALIPVDNARRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTDYAISLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTLHRMDIVQLNGRPGTRAPHFIGMYEEKEVSVLDLLGNDFVLLTGAENDSWAESAHTVSAKLGVNI
KVYRVGLSGDFIAQENIFSELYGLSDGGAVLIRPDAFIGWRSEKEVVNLDVVLEEVMSNLLCDF

Sequences:

>Translated_544_residues
MEDKYMESNDVPVLIVGGGLSGLASALFLAKHNIEYLLIERHPSTAIHPKAGGITFRTMELFRELGLEQRIRSAGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARILGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADVSEFMQGDAFGFSMVLHPEVL
GALIPVDNARRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTDYAISLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTLHRMDIVQLNGRPGTRAPHFIGMYEEKEVSVLDLLGNDFVLLTGAENDSWAESAHTVSAKLGVNI
KVYRVGLSGDFIAQENIFSELYGLSDGGAVLIRPDAFIGWRSEKEVVNLDVVLEEVMSNLLCDF
>Mature_544_residues
MEDKYMESNDVPVLIVGGGLSGLASALFLAKHNIEYLLIERHPSTAIHPKAGGITFRTMELFRELGLEQRIRSAGKTLEN
CRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEISPSKQTACYQITLEEIMLQEARILGGELSFYHELVSYEQNE
QGVIATIRNRETEKESVIHCDYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADVSEFMQGDAFGFSMVLHPEVL
GALIPVDNARRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAAESTATKFQDNRIFLVGDSAH
IMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETYHEERYPVAKLTTDYAISLLFRAANREEGSLNKMDGLAVTA
GYHYSSDAIIDDAVTLHRMDIVQLNGRPGTRAPHFIGMYEEKEVSVLDLLGNDFVLLTGAENDSWAESAHTVSAKLGVNI
KVYRVGLSGDFIAQENIFSELYGLSDGGAVLIRPDAFIGWRSEKEVVNLDVVLEEVMSNLLCDF

Specific function: Hydroxylates tetracenomycin A2 at positions C-4, C-4A, and C12A to give tetracenomycin C (TCM C). Hydroxylates as well 9- carboxymethyl-TCM B3 to yield 8-demethyl-TMC C, a side reaction [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=403, Percent_Identity=25.3101736972705, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 60429; Mature: 60429

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKYMESNDVPVLIVGGGLSGLASALFLAKHNIEYLLIERHPSTAIHPKAGGITFRTME
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEHHHHH
LFRELGLEQRIRSAGKTLENCRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEIS
HHHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHCCCCHHHHHHHHHHCC
PSKQTACYQITLEEIMLQEARILGGELSFYHELVSYEQNEQGVIATIRNRETEKESVIHC
CCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
DYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADVSEFMQGDAFGFSMVLHPEVL
EEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCEEEEECHHHH
GALIPVDNARRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAA
HEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
ESTATKFQDNRIFLVGDSAHIMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETY
CHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
HEERYPVAKLTTDYAISLLFRAANREEGSLNKMDGLAVTAGYHYSSDAIIDDAVTLHRMD
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEECCCCEECCHHEEEEEE
IVQLNGRPGTRAPHFIGMYEEKEVSVLDLLGNDFVLLTGAENDSWAESAHTVSAKLGVNI
EEEECCCCCCCCCCEEECCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHEEEEEECCEE
KVYRVGLSGDFIAQENIFSELYGLSDGGAVLIRPDAFIGWRSEKEVVNLDVVLEEVMSNL
EEEEEECCCCCEEHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCEEHHHHHHHHHHHHH
LCDF
CCCC
>Mature Secondary Structure
MEDKYMESNDVPVLIVGGGLSGLASALFLAKHNIEYLLIERHPSTAIHPKAGGITFRTME
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEHHHHH
LFRELGLEQRIRSAGKTLENCRGRIAVHTIAEANQEELAQMRANQYENDEKLLQKIEEIS
HHHHCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHCCCCHHHHHHHHHHCC
PSKQTACYQITLEEIMLQEARILGGELSFYHELVSYEQNEQGVIATIRNRETEKESVIHC
CCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEE
DYVIAADGAKSKIREQLGISAEGRGTIGGYYMNIYFEADVSEFMQGDAFGFSMVLHPEVL
EEEEECCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHCCCCCCEEEEECHHHH
GALIPVDNARRWIYHVSYDPLKGERPEDFTIERCKQIIQTAIGSTNVEPEIVSVLPWKAA
HEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
ESTATKFQDNRIFLVGDSAHIMPPTGGFGSNTGIQDAHNLAWKLAAVIKGKAKPKLLETY
CHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
HEERYPVAKLTTDYAISLLFRAANREEGSLNKMDGLAVTAGYHYSSDAIIDDAVTLHRMD
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEECCCCEECCHHEEEEEE
IVQLNGRPGTRAPHFIGMYEEKEVSVLDLLGNDFVLLTGAENDSWAESAHTVSAKLGVNI
EEEECCCCCCCCCCEEECCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHEEEEEECCEE
KVYRVGLSGDFIAQENIFSELYGLSDGGAVLIRPDAFIGWRSEKEVVNLDVVLEEVMSNL
EEEEEECCCCCEEHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCEEHHHHHHHHHHHHH
LCDF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]