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The map label for this gene is hisS [H]

Identifier: 49186135

GI number: 49186135

Start: 3108436

End: 3109713

Strand: Direct

Name: hisS [H]

Synonym: BAS3130

Alternate gene names: 49186135

Gene position: 3108436-3109713 (Clockwise)

Preceding gene: 49186128

Following gene: 49186136

Centisome position: 59.45

GC content: 37.32

Gene sequence:

>1278_bases
ATGGAAATAAGAAATGTAAAAGGAACGAAAGACTATTTACCAGAGGAGCAAGTACTGCGAAGTAAAATTAAAAGAGCGTG
TGAGGATACGTTTGAACGTTATGGCTGTAAACCGTTAGAGACACCAACGTTAAATATGTATGAGCTTATGTCATACAAGT
ACGGCGGTGGTGATGAAATATTAAAAGAAATATATACACTTCAGGATCAAGGAAAACGTGACCTTGCCTTACGCTACGAT
TTAACCATTCCATTCGCAAAAGTTGTGGCAATGAATCCGAACATCCGCCTTCCTTTTAAACGGTATGAAATTGGGAAAGT
ATTTCGAGATGGTCCTATTAAACAAGGGAGATTTCGTGAGTTTATACAATGCGACGTTGATATAGTCGGTGTTGAGTCTG
TCATGGCAGAAGCTGAACTTATGAGTATGGCGTTTGAGCTGTTCCAAACGTTAAACTTAGAAGTAACAATCCAATATAAT
AACCGCAAATTGTTAAATGGTATTCTTCAGGCCATTAACATCCCTACTGAATTAACAAGTGACGTCATTTTATCATTAGA
TAAAATCGAAAAGATTGGGATTGATGGCGTACGAAAAGATGTATTAGAGCGCGGAATTTCTGAAGAAATGGCTGATACGA
TATGTAATACCGTATTATCTTGTCTACAGCTTTCAATTGCTGACTTTAAAGAAGCTTTCAATAATCCACTCGTTGCAGAT
GGGGTAAACGAATTACAACAATTACAGCAATATTTAATCGCTCTTGGAATAAATGAGAATACGATATTTAATCCATTTTT
AGCACGAGGACTGACAATGTACACAGGTACTGTATATGAAATCTTTTTAAAAGATGGCTCGATTACATCTAGCATCGGTA
GCGGTGGTCGTTACGATAATATTATTGGAGCATTCCGTGGTGATGATATGAGCTATCCAACAGTCGGTATTTCATTCGGC
TTAGACGTTATTTATACAGCACTATCACAGAAAGAAACGATATCATCTACAGCGGATGTATTTATCATCCCGCTCGGGAC
AGAATTGCAATGCTTACAAATCGCCCAGCAATTACGTTCTACCACTTCCTTAAAAATCGAACTCGAACTAGCGGGACGCA
AATTAAAACGTGCCCTGAATTATGCCAATAAAGAAAATATCCCTTATGTGCTTATTATTGGGGAAGAAGAAATATGTACA
GAAACCGTTATGCTACGGAATATGAAGGAAGGTAGTGAAGTGAAGGTTCCCCTTTCTTCTTTAAGTAATTATTTATAA

Upstream 100 bases:

>100_bases
GGTACAGGGCTTTTCCTGTTGACCCACGAAGCGTTCATAAAGCTATGTGGGTATTTTTATACCTGCATAGCTTTTTTTAA
TTTAAAAAGGAGTGATTATG

Downstream 100 bases:

>100_bases
TACAAACCTCCCTCGTTTGGGTGGTTTTTCTATTTTATAGATATCCCACAATTAGTTAACATAAAAAAATTACATTCTAC
TAAATTCTACAAATATATAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase 1; HisRS 1 [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MEIRNVKGTKDYLPEEQVLRSKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEILKEIYTLQDQGKRDLALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFQTLNLEVTIQYN
NRKLLNGILQAINIPTELTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLQLSIADFKEAFNNPLVAD
GVNELQQLQQYLIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDDMSYPTVGISFG
LDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQQLRSTTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICT
ETVMLRNMKEGSEVKVPLSSLSNYL

Sequences:

>Translated_425_residues
MEIRNVKGTKDYLPEEQVLRSKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEILKEIYTLQDQGKRDLALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFQTLNLEVTIQYN
NRKLLNGILQAINIPTELTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLQLSIADFKEAFNNPLVAD
GVNELQQLQQYLIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDDMSYPTVGISFG
LDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQQLRSTTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICT
ETVMLRNMKEGSEVKVPLSSLSNYL
>Mature_425_residues
MEIRNVKGTKDYLPEEQVLRSKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEILKEIYTLQDQGKRDLALRYD
LTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFREFIQCDVDIVGVESVMAEAELMSMAFELFQTLNLEVTIQYN
NRKLLNGILQAINIPTELTSDVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLQLSIADFKEAFNNPLVAD
GVNELQQLQQYLIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDNIIGAFRGDDMSYPTVGISFG
LDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQQLRSTTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICT
ETVMLRNMKEGSEVKVPLSSLSNYL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=442, Percent_Identity=32.8054298642534, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI6996014, Length=443, Percent_Identity=32.0541760722348, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI65287717, Length=428, Percent_Identity=27.1028037383178, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788861, Length=456, Percent_Identity=22.1491228070175, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=456, Percent_Identity=31.140350877193, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI71993686, Length=456, Percent_Identity=31.140350877193, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6325290, Length=445, Percent_Identity=32.3595505617978, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24643061, Length=461, Percent_Identity=32.3210412147505, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24643059, Length=480, Percent_Identity=31.0416666666667, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47821; Mature: 47821

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRNVKGTKDYLPEEQVLRSKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEI
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHH
LKEIYTLQDQGKRDLALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFRE
HHHHHHHHCCCCCCEEEEEECCCCHHHEEEECCCEECCHHHHHHHHHHCCCCCCCHHHHH
FIQCDVDIVGVESVMAEAELMSMAFELFQTLNLEVTIQYNNRKLLNGILQAINIPTELTS
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCHHHHH
DVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLQLSIADFKEAFNNPLVAD
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVNELQQLQQYLIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDN
CHHHHHHHHHHHHHHCCCCCCEECHHHHCCHHHHCCEEEEEEEECCCEECCCCCCCCCCC
IIGAFRGDDMSYPTVGISFGLDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQQLRS
HHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
TTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICTETVMLRNMKEGSEVKVPLSS
CCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEHHH
LSNYL
HCCCC
>Mature Secondary Structure
MEIRNVKGTKDYLPEEQVLRSKIKRACEDTFERYGCKPLETPTLNMYELMSYKYGGGDEI
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHHH
LKEIYTLQDQGKRDLALRYDLTIPFAKVVAMNPNIRLPFKRYEIGKVFRDGPIKQGRFRE
HHHHHHHHCCCCCCEEEEEECCCCHHHEEEECCCEECCHHHHHHHHHHCCCCCCCHHHHH
FIQCDVDIVGVESVMAEAELMSMAFELFQTLNLEVTIQYNNRKLLNGILQAINIPTELTS
HHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCHHHHH
DVILSLDKIEKIGIDGVRKDVLERGISEEMADTICNTVLSCLQLSIADFKEAFNNPLVAD
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVNELQQLQQYLIALGINENTIFNPFLARGLTMYTGTVYEIFLKDGSITSSIGSGGRYDN
CHHHHHHHHHHHHHHCCCCCCEECHHHHCCHHHHCCEEEEEEEECCCEECCCCCCCCCCC
IIGAFRGDDMSYPTVGISFGLDVIYTALSQKETISSTADVFIIPLGTELQCLQIAQQLRS
HHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHC
TTSLKIELELAGRKLKRALNYANKENIPYVLIIGEEEICTETVMLRNMKEGSEVKVPLSS
CCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEHHH
LSNYL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA