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The map label for this gene is yjfC [H]

Identifier: 49185730

GI number: 49185730

Start: 2701773

End: 2703020

Strand: Reverse

Name: yjfC [H]

Synonym: BAS2724

Alternate gene names: 49185730

Gene position: 2703020-2701773 (Counterclockwise)

Preceding gene: 49185731

Following gene: 49185728

Centisome position: 51.7

GC content: 34.38

Gene sequence:

>1248_bases
ATGTATACAGAACAAGAACAGAAAGATTATATGAAAGTATGGTTTTCACTTGCAGAAGAGGCTGGTCGGAATGGGTTTAC
TTGGCCGAGTCTATTAGAAAATGAAGAATGGAATCAATATATGGCAACAGGTATGTATCGTATGCCAATAAAAACTTATA
CTGCTATTTCAAAAGCGACAGAAGAAATTATGTATGTGCTATATAGAACGTATCAATATATTCTCAATACGTCAAAAGAT
TTTCAAAAACTAGGATTTCCAGCTGAAACGTGGGAAATTGCGAGAATGAAACATACTGGTTTGTTTTCATATTTTACAAG
GTTTGACTTTATCGTAAATAGTGAAGATATAAAGTTAATAGAAGTAAATTGTGATACACCAACAGGTTATTTAGAACCGT
CTGTCGCAAATGAAGTGTTATGTCGTTATCACGATGTGAATCATCCAAATCATATAGAAGAGCATATTGTGCAGGCGTGG
GAACAAATTAAACATGACTATAGTATAGATCCTAGAGAAACGATTTATTTTACGAGTTATGATTGGCATGATGAAGACCA
TCAAACGGTTCAATTTTTAAGAAGCTATTGCTTAGATCAGTCAACGGATTATATAGGTATACAAGATATCGTCGTGGCAG
ATGATGGTATATATACGCCAAATGGTGAGAGAATTCATTATTTATATAGACTATATCCAATTGAATATTTAGTATCAGAC
GCTGATAAAAACGGGAAAAGAATTGGACTTCAGTTTTTAGATCATATAGCGCAAGGTAGAGTGAAAATCATTAATCCACC
AGCTGCTTTTCTTATGCAAAATAAAAGTGTACTCGCATTAATTTGGCAACTTTACGAAGAGGAAGTTTTCTTTGAGGAAG
AGGAGCGAGGAATCATTCAGAACTACTTCTTACCGACATATTTTACAAATAAACCGTTTATAGAAAGAAATGAATCATAT
GTTTCTAAACCTCTATACGGCCGTGAAGGCGGAGGAGTATCTATATACGAAAATAATGAACTATTAGCTGAAGATAAAAC
AGAGTATTACTTTGAACAGCGAAAAATATATCAGCAATACGTAGAAATGCCTGACTATACGATTGACACATGGGACGGTC
CGTATACTGGTAAACTATTAATTGGTTCACACTGTATTAGCGGGAGAGCTGCTGGTTTATTTTTACGTGTAGGTGAGAAA
ATAACGGGAAACTTATCGATGTTTACTGGAGTTACAATTGAAGGATAA

Upstream 100 bases:

>100_bases
AATCATCTACGACACAAACGCCGAAAGTGAATTCTGGTTCTAGTGGAATTGGGAACGCAAAAGCGCCAGCAGGATCCTAA
AATGAAAGGATAATGGAGTA

Downstream 100 bases:

>100_bases
ATGAAACAAAGAGCTTATCATTTTTGATGAGCTCTTTGTTCATATAGCGTTTTTATTGCTGTGATGTAACGCTTTCATTT
TTCTTCATTTCAAAGATTTT

Product: glutathionylspermidine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MYTEQEQKDYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNSEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIERNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Sequences:

>Translated_415_residues
MYTEQEQKDYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNSEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIERNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG
>Mature_415_residues
MYTEQEQKDYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKATEEIMYVLYRTYQYILNTSKD
FQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNSEDIKLIEVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAW
EQIKHDYSIDPRETIYFTSYDWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQNYFLPTYFTNKPFIERNESY
VSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQYVEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEK
ITGNLSMFTGVTIEG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=418, Percent_Identity=26.0765550239234, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789416, Length=406, Percent_Identity=23.1527093596059, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 48819; Mature: 48819

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEQEQKDYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTSKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNSEDIKLI
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPRETIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEECCCCCEEEEEEEEHHHHHHHHC
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQ
CCCCCCEEHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCHHH
NYFLPTYFTNKPFIERNESYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
VEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MYTEQEQKDYMKVWFSLAEEAGRNGFTWPSLLENEEWNQYMATGMYRMPIKTYTAISKAT
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCEEECCHHHHHHHHHHH
EEIMYVLYRTYQYILNTSKDFQKLGFPAETWEIARMKHTGLFSYFTRFDFIVNSEDIKLI
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
EVNCDTPTGYLEPSVANEVLCRYHDVNHPNHIEEHIVQAWEQIKHDYSIDPRETIYFTSY
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
DWHDEDHQTVQFLRSYCLDQSTDYIGIQDIVVADDGIYTPNGERIHYLYRLYPIEYLVSD
CCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEECCCCCEEEEEEEEHHHHHHHHC
ADKNGKRIGLQFLDHIAQGRVKIINPPAAFLMQNKSVLALIWQLYEEEVFFEEEERGIIQ
CCCCCCEEHHHHHHHHHCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHCHHHCCHHH
NYFLPTYFTNKPFIERNESYVSKPLYGREGGGVSIYENNELLAEDKTEYYFEQRKIYQQY
HCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHHHH
VEMPDYTIDTWDGPYTGKLLIGSHCISGRAAGLFLRVGEKITGNLSMFTGVTIEG
HCCCCCEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]