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The map label for this gene is ykfB [H]

Identifier: 49185666

GI number: 49185666

Start: 2644522

End: 2645574

Strand: Reverse

Name: ykfB [H]

Synonym: BAS2659

Alternate gene names: 49185666

Gene position: 2645574-2644522 (Counterclockwise)

Preceding gene: 49185667

Following gene: 49185665

Centisome position: 50.6

GC content: 37.61

Gene sequence:

>1053_bases
ATGAAAATTACCGATGTAAAAGTAAATCGTAGACGTGTAAATCTTCATACACCGTTTAAAACCGCGCTTCGTACCGTAAC
CGAAATAGAAAGTATAGATGTATATATTCATACAGATGAAGGAATTGTCGGTAAGGGAGCTGGAGCTGCAACGCCGGTTA
TTACGGGTGATTTCGCTAACGGGATTGAAGAAGCAATTTTAGGGCCGATGCGTTCAAGTTTAATTGGTCAAGATATCATT
CAATTTCAGCAGTTACTGCAGCACATTCAAATGAGTTGCATTGGAAATCCAAGTGCGAAAGCAGCGGTAGATATCGCATT
ATATGATGTGTATTGTCAATATCATAACATTCCACTTTACGCATTATTAGGCGGGAAGAAAGAAATTCATACAGATATTA
CGTTGAGTGTAGATGAGCCTTTCATTATGGCGAAAGAGGCAAAGCAACATGTAGAAAAAGGATTTCAAACTTTAAAAATT
AAAGTGGGGAAATCTGCACACTTAGACTTAGAACGTATTGAAGCGATTCGAAATAGCGTACCAAAAAATACGACGTTACG
ATTAGATGCAAATCAAGGGTGGAATCCAAAAGAGGCAGTTTCTATCATTAAAGAGATGGAAAATCGCAATTTAAATATTG
AATTTATTGAACAACCTGTTCATGCGAAAGATTGGGATGGATTAAAGTACGTCAAAGATCATGTACAAACACCTATTATG
GCAGATGAAAGCATATTTTCAGCGAGTGATGCATTAAAAATTGTGCAAGGAAGATATGTAGACTTAATCAATATTAAATT
AATGAAATGTAGTGGCATTCGCGAAGCGTGGCGTATTGCCGATATCGCAGAAGCAGCTGGTGTGAAATGTATGGTAGGAA
GCATGATGGAATCTTCTCTTTCTGTTAGTGCTGTTGCACATTTAGCTGCTGCACATCCTAATATTCATTACTTCGATCTT
GATGCGCCACTTTGGTTAATGGAAGAACCGGAAGGAATGACTTATTCTGGATCAAAGGTAAACCTTCAATCGACAGTGAA
TAGTAAATCTTAG

Upstream 100 bases:

>100_bases
GTACCTTCTACATGGAAAGTAAGCACAACCGGAGATCTTTTGATTCAAAAAAGTTAAGAATTAAGGCTCTATTTTGCTAG
ATAAAGAAAGGATGAACAAA

Downstream 100 bases:

>100_bases
GAGAAGAGACTAGTAAATTATCTTTTAAGGGGGATATTTATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTAT
ATTTTCATCGTTTACATCAG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMRSSLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYHNIPLYALLGGKKEIHTDITLSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYVDLINIKLMKCSGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Sequences:

>Translated_350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMRSSLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYHNIPLYALLGGKKEIHTDITLSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYVDLINIKLMKCSGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
>Mature_350_residues
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFANGIEEAILGPMRSSLIGQDII
QFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYHNIPLYALLGGKKEIHTDITLSVDEPFIMAKEAKQHVEKGFQTLKI
KVGKSAHLDLERIEAIRNSVPKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
ADESIFSASDALKIVQGRYVDLINIKLMKCSGIREAWRIADIAEAAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHYFDL
DAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=331, Percent_Identity=32.02416918429, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI48994953, Length=309, Percent_Identity=29.126213592233, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 38748; Mature: 38748

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFAN
CCEEEEEECCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEECCCCCCCCEEECHHHH
GIEEAILGPMRSSLIGQDIIQFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYHNIPLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHCCCEEE
ALLGGKKEIHTDITLSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLERIEAIRNSV
EEECCCEEECEEEEEECCCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
PKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
ADESIFSASDALKIVQGRYVDLINIKLMKCSGIREAWRIADIAEAAGVKCMVGSMMESSL
CCCHHHHHHHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC
>Mature Secondary Structure
MKITDVKVNRRRVNLHTPFKTALRTVTEIESIDVYIHTDEGIVGKGAGAATPVITGDFAN
CCEEEEEECCEEEEECCCHHHHHHHHHHHEEEEEEEECCCCEEECCCCCCCCEEECHHHH
GIEEAILGPMRSSLIGQDIIQFQQLLQHIQMSCIGNPSAKAAVDIALYDVYCQYHNIPLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHHCCCEEE
ALLGGKKEIHTDITLSVDEPFIMAKEAKQHVEKGFQTLKIKVGKSAHLDLERIEAIRNSV
EEECCCEEECEEEEEECCCCEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCC
PKNTTLRLDANQGWNPKEAVSIIKEMENRNLNIEFIEQPVHAKDWDGLKYVKDHVQTPIM
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCC
ADESIFSASDALKIVQGRYVDLINIKLMKCSGIREAWRIADIAEAAGVKCMVGSMMESSL
CCCHHHHHHHHHHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
SVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]