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The map label for this gene is ooxA [H]

Identifier: 49185655

GI number: 49185655

Start: 2634948

End: 2636183

Strand: Direct

Name: ooxA [H]

Synonym: BAS2648

Alternate gene names: 49185655

Gene position: 2634948-2636183 (Clockwise)

Preceding gene: 49185654

Following gene: 49185658

Centisome position: 50.39

GC content: 40.78

Gene sequence:

>1236_bases
ATGAATGATGTCATAATTATCGGAGCTGGACCAGCGGGTTTATCAGCCTCTATCTCTTGTGCTCGTTTTGGACTAAATGT
ACTTGTTATTGATGAATTTATGAAGCCTGGCGGGAGATTGTTAGGACAATTACATCAAGAGCCTTCTGGAGAATGGTGGA
ACGGAATAGAAGAATCGAAGCGACTTCAAGAAGAAGCAAAATCACTTTCAGTTCATATTCGATGTGGTATTTCCGTCTAT
AATTTAGATAAAGATGAAAGTTGTTGGTTCGTACATACGAATATCGGTACGTTAGAAGCACCGTTCGTTTTACTTGCTAC
TGGCGCTGCTGAGTACTCCATTCCCCTTCCCGATTGGACACTTCCAGGAGTCATGTCAATCGGGGCAGCCCAAGTAATGA
CAAATGTTCATCGCGTTCAAGTTGGGGAAAAAGGAATCATTATTGGTGCTAACATTTTGTCATTCGCTATTTTGAATGAA
TTACAATTAGCAGGCATCAAAGTTGAACATATCGTACTTCCTGAAAAAAGTGAATTAAGTCAAAAGGCTGGAGAACCAGA
AGTAGTTTTAAACTCTCTTTTAAATGCGGCTCATCTCGCTCCTTCTGCTTTTCTACGCATAGGTAGCCATTTTATGAAAT
ATGACTGGATTCGAAAAGCCGGATTAACCTTCTATCCAAACAGCGGAATGAAAATAAACGATACACCTCTTCACCTTCGA
AAAGCAGCGCTTGAAATTATCGGAACAGATCAAGTTGAAGGTGTACGCGTTGCTAATATTGATACGAAAGGAAACATCAT
CACTGGATCGGAACAAATATATGAGGCAGACTTCGTTTGTATTGCAGGTGGCTTATATCCTCTTGCTGAACTAGCCGCTG
TAGCCGGCTGTCCTTTCCGTTACATTCCGGAATTAGGTGGGCACGTTCCTCTCCATTCTGAAACAATGGAAACCCCTCTT
ACTGGTTTATTTGTAGCCGGCAATATAACTGGTATTGAAAGTGGGAAAATTGCAATGGCACAAGGTACTGTTGCTGGATA
TTCAATTGTAAAACAGGCAAATAAAAAATCCCATTCAGTTGAACAACAATTGCAACAAGCAATCCAACATGTACATGCCG
TACGTCAAGAAGCTGCCATTCAATTTAACCAGGGTGTTGATGTCGGTAGAAGGAAGATGAATGAAATTTGGCGTGATTAT
TCCCTTGCATTTGCACATACTAAAAAGAGCTGTTGA

Upstream 100 bases:

>100_bases
AAGCAAACGTACGAGCATGCTTAACTGTTGTAGAAAACGGTATGGTTGTTGATAGTGGTAAACAGCATCCAAATATCGTT
AGAGAGATGGTGAAAAAGCG

Downstream 100 bases:

>100_bases
GAATACTCTCAACAGCTCTTTTTTCTACTCATCTGAGTTATTAAATTTCGCCAATGCGTAAATGCCTTCTTCATCATCTA
ATTCAAGTTGTAATCTTGCC

Product: sarcosine oxidase subunit alpha, C-terminal

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit A [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNDVIIIGAGPAGLSASISCARFGLNVLVIDEFMKPGGRLLGQLHQEPSGEWWNGIEESKRLQEEAKSLSVHIRCGISVY
NLDKDESCWFVHTNIGTLEAPFVLLATGAAEYSIPLPDWTLPGVMSIGAAQVMTNVHRVQVGEKGIIIGANILSFAILNE
LQLAGIKVEHIVLPEKSELSQKAGEPEVVLNSLLNAAHLAPSAFLRIGSHFMKYDWIRKAGLTFYPNSGMKINDTPLHLR
KAALEIIGTDQVEGVRVANIDTKGNIITGSEQIYEADFVCIAGGLYPLAELAAVAGCPFRYIPELGGHVPLHSETMETPL
TGLFVAGNITGIESGKIAMAQGTVAGYSIVKQANKKSHSVEQQLQQAIQHVHAVRQEAAIQFNQGVDVGRRKMNEIWRDY
SLAFAHTKKSC

Sequences:

>Translated_411_residues
MNDVIIIGAGPAGLSASISCARFGLNVLVIDEFMKPGGRLLGQLHQEPSGEWWNGIEESKRLQEEAKSLSVHIRCGISVY
NLDKDESCWFVHTNIGTLEAPFVLLATGAAEYSIPLPDWTLPGVMSIGAAQVMTNVHRVQVGEKGIIIGANILSFAILNE
LQLAGIKVEHIVLPEKSELSQKAGEPEVVLNSLLNAAHLAPSAFLRIGSHFMKYDWIRKAGLTFYPNSGMKINDTPLHLR
KAALEIIGTDQVEGVRVANIDTKGNIITGSEQIYEADFVCIAGGLYPLAELAAVAGCPFRYIPELGGHVPLHSETMETPL
TGLFVAGNITGIESGKIAMAQGTVAGYSIVKQANKKSHSVEQQLQQAIQHVHAVRQEAAIQFNQGVDVGRRKMNEIWRDY
SLAFAHTKKSC
>Mature_411_residues
MNDVIIIGAGPAGLSASISCARFGLNVLVIDEFMKPGGRLLGQLHQEPSGEWWNGIEESKRLQEEAKSLSVHIRCGISVY
NLDKDESCWFVHTNIGTLEAPFVLLATGAAEYSIPLPDWTLPGVMSIGAAQVMTNVHRVQVGEKGIIIGANILSFAILNE
LQLAGIKVEHIVLPEKSELSQKAGEPEVVLNSLLNAAHLAPSAFLRIGSHFMKYDWIRKAGLTFYPNSGMKINDTPLHLR
KAALEIIGTDQVEGVRVANIDTKGNIITGSEQIYEADFVCIAGGLYPLAELAAVAGCPFRYIPELGGHVPLHSETMETPL
TGLFVAGNITGIESGKIAMAQGTVAGYSIVKQANKKSHSVEQQLQQAIQHVHAVRQEAAIQFNQGVDVGRRKMNEIWRDY
SLAFAHTKKSC

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To T-protein and to dimethylglycine dehydrogenase [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR017224
- InterPro:   IPR000103 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF07992 Pyr_redox_2 [H]

EC number: 1.5.3.1

Molecular weight: Translated: 44550; Mature: 44550

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVIIIGAGPAGLSASISCARFGLNVLVIDEFMKPGGRLLGQLHQEPSGEWWNGIEESK
CCCEEEEECCCCCCCCCCCHHHHCCEEEEEEHHHCCHHHHHHHHHCCCCCCHHCCHHHHH
RLQEEAKSLSVHIRCGISVYNLDKDESCWFVHTNIGTLEAPFVLLATGAAEYSIPLPDWT
HHHHHHHCEEEEEEECEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEECCCCCCC
LPGVMSIGAAQVMTNVHRVQVGEKGIIIGANILSFAILNELQLAGIKVEHIVLPEKSELS
CCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHCCEEEEEEECCCHHHHH
QKAGEPEVVLNSLLNAAHLAPSAFLRIGSHFMKYDWIRKAGLTFYPNSGMKINDTPLHLR
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCHHHH
KAALEIIGTDQVEGVRVANIDTKGNIITGSEQIYEADFVCIAGGLYPLAELAAVAGCPFR
HHHHHHHCCCCCCCEEEEEECCCCCEEECCHHHEECCEEEEECCHHHHHHHHHHHCCCHH
YIPELGGHVPLHSETMETPLTGLFVAGNITGIESGKIAMAQGTVAGYSIVKQANKKSHSV
HHHHCCCCCCCCCCHHCCCCCEEEEECCEEECCCCCEEEECCCHHHHHHHHHHCCHHHHH
EQQLQQAIQHVHAVRQEAAIQFNQGVDVGRRKMNEIWRDYSLAFAHTKKSC
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MNDVIIIGAGPAGLSASISCARFGLNVLVIDEFMKPGGRLLGQLHQEPSGEWWNGIEESK
CCCEEEEECCCCCCCCCCCHHHHCCEEEEEEHHHCCHHHHHHHHHCCCCCCHHCCHHHHH
RLQEEAKSLSVHIRCGISVYNLDKDESCWFVHTNIGTLEAPFVLLATGAAEYSIPLPDWT
HHHHHHHCEEEEEEECEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEECCCCCCC
LPGVMSIGAAQVMTNVHRVQVGEKGIIIGANILSFAILNELQLAGIKVEHIVLPEKSELS
CCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHCCEEEEEEECCCHHHHH
QKAGEPEVVLNSLLNAAHLAPSAFLRIGSHFMKYDWIRKAGLTFYPNSGMKINDTPLHLR
HHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCHHHH
KAALEIIGTDQVEGVRVANIDTKGNIITGSEQIYEADFVCIAGGLYPLAELAAVAGCPFR
HHHHHHHCCCCCCCEEEEEECCCCCEEECCHHHEECCEEEEECCHHHHHHHHHHHCCCHH
YIPELGGHVPLHSETMETPLTGLFVAGNITGIESGKIAMAQGTVAGYSIVKQANKKSHSV
HHHHCCCCCCCCCCHHCCCCCEEEEECCEEECCCCCEEEECCCHHHHHHHHHHCCHHHHH
EQQLQQAIQHVHAVRQEAAIQFNQGVDVGRRKMNEIWRDYSLAFAHTKKSC
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA