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The map label for this gene is kinE [H]

Identifier: 49185464

GI number: 49185464

Start: 2453135

End: 2454664

Strand: Direct

Name: kinE [H]

Synonym: BAS2456

Alternate gene names: 49185464

Gene position: 2453135-2454664 (Clockwise)

Preceding gene: 49185463

Following gene: 49185465

Centisome position: 46.92

GC content: 31.05

Gene sequence:

>1530_bases
ATGCTTTCAAATCTAATTATAGATGGAGCAATGAAATGCTTTCAAAGTAAAAATACAACATTACTTACTCGAAAAGAGGA
GGAACTACATACAATCCTACAAAACATGCCTGTTATGGTTGATGCGCTTGATTATAATGGAGACTTTGTTTTGTGGAATC
GCGAATGTGAAATTGTAACAGGATATACTGCGGAAGAGGTAATTGGAAATCCAGACGCACTTCAATTACTGTATCCTGAT
CATAATTATCGTCATGAGATACAAAAGAAATTTTTGACTCGTGGAAAGAATTTTAGAGATTGGGAAATGCGCTTAACATG
TAAAAATGGTGAAATTAAAACGATAATGTGGTCCAATATATCAGAACAGTTTCCTGTAAGTGGATTTAGCTATTGGGCTG
TTGGTGTAGATATTACACATTTAAAAGCAATAGAAGAGAAATTAAAACAACAAACGTCTGAATTGGAATTAATTTTTCAA
GCTTTACCAGATTTATGTTTCTTAACAGAAGATGATGGAACAATTATTGATTATAAAGCAGGATCCCCCACAAAATTTTA
CGTACCCGCAGAAGCTTTTATGGGAAAAAAGTTTTACGAAGTATTACCATCTTCAGTAGCACAAAAACTTCAAGAAGCAA
TTTGTCAAGTAAAAGAAAAAGGAACAAATGTAATTGTTGAATATCCACTTACAATTAATGAAAGTATCGATTTCTTTGAA
GCTAGATGTTTACCTTTATTGCATGATAAAATTATGATTATTGTACGGGATATTACAGAGCGAAAAAAGACAGAAGAATT
GCTAAATAAATCAGATACACTTGCAGCAATCGGTCAATTAGCAGCTGGTGTAGCTCATGAAGTTAGGAATCCATTAACAG
TCATTAAAGGGTTTATACAGTTATTCCAAATTAATAAAGAAGATCAAGAAAAATATTTTGATCTAATGCTTTCTGAAATT
GAAAGAATAGAAGCGATTCTTCAAGAGTTTTTGTCGATTGCTAAAACAGACGAAATAAGTACCGAAAAGAAAAATATTTA
TCAAATATTTAAAAATGTCGTCTCATTAATAAATACAAAAGCAATTATGACAAACATTCAAGTTGAATTATATACAGATT
CCAAAGATATAATCATTGAATGCTCAGAAAATCAACTGAAACAAGTATTTATTAACATTTTGCAAAATTCGATCGAAGCT
ATGCCAGATGGCGGAAGAATTTCAATTCACATAAAAGAAATAGGTAAAGATGGTATCATTATTAGTGTAATTGATAAAGG
AATAGGAATACCCGCAGAAAGAATTAAAAGATTAGGTGAACCGTTCTATAGTACGAAAGAAAAAGGTACCGGTATTGGAT
TGATGTTAAGTTATAAAATTATTGAAAGTCATCAAGGGAATATAAGTATTATGAGTGAGGTTGGTGTCGGGACAACAGTA
ACGATTTACTTGCCAAAAATTCAGAGTAAAAAGCCTTTATCAAATTATGGTGATAGATTAGAATCAATACGTTCAATTAT
TAACTCTTAA

Upstream 100 bases:

>100_bases
CCAAAATTGAAATCCCCATTTTTTATACAATCAATATTTAGGATCTTTTGTTGTATGGTCTCAGAAAAGCGAAATTTTCA
ATTGCCAAAAATACTTTGAA

Downstream 100 bases:

>100_bases
ATTATTAAATACCCAATAACGAAAAGTTAAGCTTTTCGTTATTGGGTATTTTTGTATTTAATTTAGAAAACTTTACCTTT
TCTTTAGATTTGCTTTAATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLSNLIIDGAMKCFQSKNTTLLTRKEEELHTILQNMPVMVDALDYNGDFVLWNRECEIVTGYTAEEVIGNPDALQLLYPD
HNYRHEIQKKFLTRGKNFRDWEMRLTCKNGEIKTIMWSNISEQFPVSGFSYWAVGVDITHLKAIEEKLKQQTSELELIFQ
ALPDLCFLTEDDGTIIDYKAGSPTKFYVPAEAFMGKKFYEVLPSSVAQKLQEAICQVKEKGTNVIVEYPLTINESIDFFE
ARCLPLLHDKIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQLFQINKEDQEKYFDLMLSEI
ERIEAILQEFLSIAKTDEISTEKKNIYQIFKNVVSLINTKAIMTNIQVELYTDSKDIIIECSENQLKQVFINILQNSIEA
MPDGGRISIHIKEIGKDGIIISVIDKGIGIPAERIKRLGEPFYSTKEKGTGIGLMLSYKIIESHQGNISIMSEVGVGTTV
TIYLPKIQSKKPLSNYGDRLESIRSIINS

Sequences:

>Translated_509_residues
MLSNLIIDGAMKCFQSKNTTLLTRKEEELHTILQNMPVMVDALDYNGDFVLWNRECEIVTGYTAEEVIGNPDALQLLYPD
HNYRHEIQKKFLTRGKNFRDWEMRLTCKNGEIKTIMWSNISEQFPVSGFSYWAVGVDITHLKAIEEKLKQQTSELELIFQ
ALPDLCFLTEDDGTIIDYKAGSPTKFYVPAEAFMGKKFYEVLPSSVAQKLQEAICQVKEKGTNVIVEYPLTINESIDFFE
ARCLPLLHDKIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQLFQINKEDQEKYFDLMLSEI
ERIEAILQEFLSIAKTDEISTEKKNIYQIFKNVVSLINTKAIMTNIQVELYTDSKDIIIECSENQLKQVFINILQNSIEA
MPDGGRISIHIKEIGKDGIIISVIDKGIGIPAERIKRLGEPFYSTKEKGTGIGLMLSYKIIESHQGNISIMSEVGVGTTV
TIYLPKIQSKKPLSNYGDRLESIRSIINS
>Mature_509_residues
MLSNLIIDGAMKCFQSKNTTLLTRKEEELHTILQNMPVMVDALDYNGDFVLWNRECEIVTGYTAEEVIGNPDALQLLYPD
HNYRHEIQKKFLTRGKNFRDWEMRLTCKNGEIKTIMWSNISEQFPVSGFSYWAVGVDITHLKAIEEKLKQQTSELELIFQ
ALPDLCFLTEDDGTIIDYKAGSPTKFYVPAEAFMGKKFYEVLPSSVAQKLQEAICQVKEKGTNVIVEYPLTINESIDFFE
ARCLPLLHDKIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQLFQINKEDQEKYFDLMLSEI
ERIEAILQEFLSIAKTDEISTEKKNIYQIFKNVVSLINTKAIMTNIQVELYTDSKDIIIECSENQLKQVFINILQNSIEA
MPDGGRISIHIKEIGKDGIIISVIDKGIGIPAERIKRLGEPFYSTKEKGTGIGLMLSYKIIESHQGNISIMSEVGVGTTV
TIYLPKIQSKKPLSNYGDRLESIRSIINS

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=339, Percent_Identity=29.7935103244838, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1790436, Length=241, Percent_Identity=31.5352697095436, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786600, Length=259, Percent_Identity=25.4826254826255, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693157, Length=322, Percent_Identity=26.0869565217391, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790300, Length=238, Percent_Identity=27.7310924369748, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788713, Length=423, Percent_Identity=23.1678486997636, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=395, Percent_Identity=23.5443037974684, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=21.5596330275229, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=21.6216216216216, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57829; Mature: 57829

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNLIIDGAMKCFQSKNTTLLTRKEEELHTILQNMPVMVDALDYNGDFVLWNRECEIVT
CCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCEEEEEEECCCCEEEECCCEEEEE
GYTAEEVIGNPDALQLLYPDHNYRHEIQKKFLTRGKNFRDWEMRLTCKNGEIKTIMWSNI
CCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCC
SEQFPVSGFSYWAVGVDITHLKAIEEKLKQQTSELELIFQALPDLCFLTEDDGTIIDYKA
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECC
GSPTKFYVPAEAFMGKKFYEVLPSSVAQKLQEAICQVKEKGTNVIVEYPLTINESIDFFE
CCCEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEECCCCHHHH
ARCLPLLHDKIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQ
HHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LFQINKEDQEKYFDLMLSEIERIEAILQEFLSIAKTDEISTEKKNIYQIFKNVVSLINTK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AIMTNIQVELYTDSKDIIIECSENQLKQVFINILQNSIEAMPDGGRISIHIKEIGKDGII
HHEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEE
ISVIDKGIGIPAERIKRLGEPFYSTKEKGTGIGLMLSYKIIESHQGNISIMSEVGVGTTV
EEEECCCCCCCHHHHHHCCCCHHCCCCCCCCEEEEEEEEEHHCCCCCEEEEEECCCCEEE
TIYLPKIQSKKPLSNYGDRLESIRSIINS
EEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSNLIIDGAMKCFQSKNTTLLTRKEEELHTILQNMPVMVDALDYNGDFVLWNRECEIVT
CCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCCEEEEEEECCCCEEEECCCEEEEE
GYTAEEVIGNPDALQLLYPDHNYRHEIQKKFLTRGKNFRDWEMRLTCKNGEIKTIMWSNI
CCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCC
SEQFPVSGFSYWAVGVDITHLKAIEEKLKQQTSELELIFQALPDLCFLTEDDGTIIDYKA
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECC
GSPTKFYVPAEAFMGKKFYEVLPSSVAQKLQEAICQVKEKGTNVIVEYPLTINESIDFFE
CCCEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEECCCCHHHH
ARCLPLLHDKIMIIVRDITERKKTEELLNKSDTLAAIGQLAAGVAHEVRNPLTVIKGFIQ
HHHHHHHHHHHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LFQINKEDQEKYFDLMLSEIERIEAILQEFLSIAKTDEISTEKKNIYQIFKNVVSLINTK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AIMTNIQVELYTDSKDIIIECSENQLKQVFINILQNSIEAMPDGGRISIHIKEIGKDGII
HHEEEEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEE
ISVIDKGIGIPAERIKRLGEPFYSTKEKGTGIGLMLSYKIIESHQGNISIMSEVGVGTTV
EEEECCCCCCCHHHHHHCCCCHHCCCCCCCCEEEEEEEEEHHCCCCCEEEEEECCCCEEE
TIYLPKIQSKKPLSNYGDRLESIRSIINS
EEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]