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The map label for this gene is nprT [H]

Identifier: 49185401

GI number: 49185401

Start: 2389823

End: 2391415

Strand: Direct

Name: nprT [H]

Synonym: BAS2393

Alternate gene names: 49185401

Gene position: 2389823-2391415 (Clockwise)

Preceding gene: 49185400

Following gene: 49185402

Centisome position: 45.71

GC content: 34.71

Gene sequence:

>1593_bases
ATGATTTTTATAAAGACAAATATAAGCGTAATAGTTTTGATAATAAGGGAAAAAATAGTATCAGTTGTACATGCATGGCA
TTCAGGAGAAACAGATGACCCTAAAAATTGGGGGAATGCATTTAGTGCTAATATTAACAATGTGAGTATGCTTATATATG
GTGATCCAATGGTTAGAGCGTTTGACATAGCGGGCCATGAATTTACACATGCGGTTACATCTAGCGAATCTAATCTTGAA
TTCTTTGGGGAGTCTGGGGCAATTAACGAGGCATTATCTGATATTATGGGAACAGCTATTGAAAAATATATAAATAATGG
GAAATTTAATTGGACAATAGGAGAACAAAGCGGATCCGTTCTCCGTGATATGAAAAATCCATCTTCAGTCAAATTTTTTG
ATGGAGTACCGTACCCAGATGATTATAGTAAATACAGTGATTTAAACGGAGAAGATAATGAGGGCGTTCATTTTAACTCA
AGTATTATTAATAAAGTCGCGTATTTAATTGCACAAGGCGGCACTCATAACGGTGTAACTGTAAATGGTATTGGTGAAGA
TAAAATGTTTGATATTTTCTATTATGCAAATACTGATGAATTGAACATGACTTCTAATTTCTCTGAATTAAGATTAGCGT
GTCTTAAAGTTGCAACAAATAAATATGGAGCAAATTCAATTGAGGTTGAAACGGTCCAAAAAGCTTTTGATGCAGCAAAA
ATTAAAGGACCAGTGAAAGAAGACGAAAAAACTGAGGTAAACCAAGTCCCAGAGTTAACTGTACCACTTACAATTACACT
ACGTGTAGGTGATACGTTTGACCCGATAAGCAATGTAAAAGCTATTGATAAAGAAGATGGTGATTTAACAACTCGAGTAA
AACATAAAGGTGATGTAGATACTTCTAAACCAGGCAAATACATTGTGGATTACTCTGTCATTGATTCTCAAGGCGGGAAT
GCAACGGCAACACAGACTGTAATTGTAGAGGGAAATGGAGAAACAGCAGATCTAAAACCTACATTAACAGTTCCTGTTGC
AACGACAATTACTGTAGGAGATTCATTCGATCCAATGGCAAAAGTAAAAGCTATTGATAAAGAAGACAGCAATCTTACTT
CTAAAGTAAAAATTGATGGTGAAGTAGATACCTCTAAAGCCGGTACATATGTATTAACGTACAAAGTTACAGATTCAAAA
GGTAATGAAGGAACTGCGAAACAAACTGTAACGGTTAAAGTTAGAGAAGAAGTTAAGAATGAAATACCAATACTACAAGT
ACCTGCTACTACTACAATTACTAAGGGAGATAAATTTGATCTAATGGTAGGAGTATCAGCTACTGATAAAGAGGACGGTG
ATCTTACTTCTAAAGTCATGTATGAAGGAACTGTAGATACCTCTAGAACTGGTACTTTTGAAATAAAATATAGTGTCAGA
GATTCTGTAGGTAATGAGGTACATACAATACAAGAAGTATTTGTGAAAGATAAAGACACTGGAAAAACTAATAGCCTTGC
TAACAACAGTAATTCTAATAAAAATGAATCTACATATAAAGAGCTTCCCAATACGGGGGGGCAGTACAACTAA

Upstream 100 bases:

>100_bases
TTTGAAGAAAGATATTATCCTGGTATATTAGCAACTAGCACTTCAACAACCTTTGATGATCCAAAGGCGGTCAGTGCTCA
TTTCTTAGCAACAAAGGTAT

Downstream 100 bases:

>100_bases
CAGTACAACAATGGGATTATGGATGGTTATTGTAGGTACAGTACTCACTTTGGCTCGTAAATTTAGAAAGATACAAAAAT
AATAAGGATTATTCGTTTTA

Product: thermolysin metallopeptidase

Products: NA

Alternate protein names: Thermostable neutral proteinase [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MIFIKTNISVIVLIIREKIVSVVHAWHSGETDDPKNWGNAFSANINNVSMLIYGDPMVRAFDIAGHEFTHAVTSSESNLE
FFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNS
SIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAFDAAK
IKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHKGDVDTSKPGKYIVDYSVIDSQGGN
ATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSK
GNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIKYSVR
DSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN

Sequences:

>Translated_530_residues
MIFIKTNISVIVLIIREKIVSVVHAWHSGETDDPKNWGNAFSANINNVSMLIYGDPMVRAFDIAGHEFTHAVTSSESNLE
FFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNS
SIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAFDAAK
IKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHKGDVDTSKPGKYIVDYSVIDSQGGN
ATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSK
GNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIKYSVR
DSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
>Mature_530_residues
MIFIKTNISVIVLIIREKIVSVVHAWHSGETDDPKNWGNAFSANINNVSMLIYGDPMVRAFDIAGHEFTHAVTSSESNLE
FFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNS
SIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAFDAAK
IKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHKGDVDTSKPGKYIVDYSVIDSQGGN
ATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSK
GNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIKYSVR
DSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN

Specific function: Extracellular zinc metalloprotease [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.27 [H]

Molecular weight: Translated: 57727; Mature: 57727

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00142 ZINC_PROTEASE ; PS50268 CADHERIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFIKTNISVIVLIIREKIVSVVHAWHSGETDDPKNWGNAFSANINNVSMLIYGDPMVRA
CEEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCEEE
FDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSV
EECCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
LRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTHNGVT
HHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCEE
VNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAFDAAK
EECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
IKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHKGDVD
CCCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCCCEEEEEECCCCCC
TSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMA
CCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCHH
KVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVREEVKN
HHEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEEHHHHHH
EIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIKYSVR
CCCEEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEC
DSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MIFIKTNISVIVLIIREKIVSVVHAWHSGETDDPKNWGNAFSANINNVSMLIYGDPMVRA
CEEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCCEEE
FDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSV
EECCCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
LRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTHNGVT
HHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCCCEE
VNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAFDAAK
EECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
IKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHKGDVD
CCCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCCCEEEEEECCCCCC
TSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMA
CCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCHH
KVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVREEVKN
HHEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEEHHHHHH
EIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIKYSVR
CCCEEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEC
DSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2993245 [H]