Click here to switch to the map view.

The map label for this gene is hly [H]

Identifier: 49185362

GI number: 49185362

Start: 2353037

End: 2353759

Strand: Direct

Name: hly [H]

Synonym: BAS2353

Alternate gene names: 49185362

Gene position: 2353037-2353759 (Clockwise)

Preceding gene: 49185361

Following gene: 49185363

Centisome position: 45.0

GC content: 33.33

Gene sequence:

>723_bases
ATGACTTCAGCAAAGGTTACTTCTGAGGTAGGGTATACACTTGGTGGGAGTGTTAAAGTTGGAGTAAACGATAAAGGGCC
AAATGCCGATGCAAGTATTACAGGTAGCTTTGCTTGGAAAGAAAGTGTATCTTATGATCAGGTAGACTATAAAACGGTAT
TAGAGACTCACACAGATAAAAAATTAAATTGGAAAGTAGGATTCCAATCGTTTAATTTCCCAGAGTGGGGAATTTATAAT
CGTGATTCATTCAATACTTTCTATGGTAATCAACTATTTATGAAATCACGTAGTTATAATGAAGGAACGAATAATTTTGT
TTCAAAAGACACAGTACCAGCTTTAACAGGATATGGTTTTTCTCCTAATGTAGTAGCGGTTATCACTGCTGATAAAACAG
AATCTACATCAGATTTAAAAATAACAAATCGTAGAATTTCAGATCAGTACAATATTGAATGGGTAAGTTCAAAATGGTGG
GGAACAAATAATAAGGATACATATAATGAGTTCTTTACAAACAACTATAAATTAGACTGGAAAAACCATCAAGTTACTCT
TGATAACCAAAAAGCCCCTGAAGAACAAATGATTGGTATCAACAACGTGAATAACCAGCTTAACAAAGGAAAAGGAAAAT
TATCTTTTTCAATGAATGGAAATCAGCTTAAGGCTACATCTAGCAATGCTGGTTATGGTATCAGTTACGAAGATAAAAAT
TGA

Upstream 100 bases:

>100_bases
CTATAACGCAGGTTTAAAATGGCCATCGGCGTATCATACGGAAGCAGAAATAACTAGTGGCGATAGCGCTCAATTCCATA
AAGCTGCCCCAGTTAATACA

Downstream 100 bases:

>100_bases
GGTATCTTTGTAAATGGTGAAAAGGTCTACACGTTTAATGAAAAAACAACTGTAGGTAATATCTCAAATGACATTAACAA
ATTAAACATCAAAGGACCTT

Product: hemolysin II, C-terminal

Products: NA

Alternate protein names: Alpha-HL; Alpha-toxin [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNFPEWGIYN
RDSFNTFYGNQLFMKSRSYNEGTNNFVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWW
GTNNKDTYNEFFTNNYKLDWKNHQVTLDNQKAPEEQMIGINNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKN

Sequences:

>Translated_240_residues
MTSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNFPEWGIYN
RDSFNTFYGNQLFMKSRSYNEGTNNFVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWW
GTNNKDTYNEFFTNNYKLDWKNHQVTLDNQKAPEEQMIGINNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKN
>Mature_239_residues
TSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSYDQVDYKTVLETHTDKKLNWKVGFQSFNFPEWGIYNR
DSFNTFYGNQLFMKSRSYNEGTNNFVSKDTVPALTGYGFSPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWG
TNNKDTYNEFFTNNYKLDWKNHQVTLDNQKAPEEQMIGINNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKN

Specific function: Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005831
- InterPro:   IPR003963
- InterPro:   IPR001340
- InterPro:   IPR016183 [H]

Pfam domain/function: PF07968 Leukocidin [H]

EC number: NA

Molecular weight: Translated: 27037; Mature: 26906

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSYDQVDYKTVLETHTDK
CCCCCCCHHCCEEECCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCC
KLNWKVGFQSFNFPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNNFVSKDTVPALTGYGF
EEEEEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCEECCCC
SPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNKDTYNEFFTNNYKLDW
CCCEEEEEEECCCCCCCCEEEECEECCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEE
KNHQVTLDNQKAPEEQMIGINNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKN
CCCEEEECCCCCCHHHEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCC
>Mature Secondary Structure 
TSAKVTSEVGYTLGGSVKVGVNDKGPNADASITGSFAWKESVSYDQVDYKTVLETHTDK
CCCCCCHHCCEEECCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHCCCCC
KLNWKVGFQSFNFPEWGIYNRDSFNTFYGNQLFMKSRSYNEGTNNFVSKDTVPALTGYGF
EEEEEECCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCEECCCC
SPNVVAVITADKTESTSDLKITNRRISDQYNIEWVSSKWWGTNNKDTYNEFFTNNYKLDW
CCCEEEEEEECCCCCCCCEEEECEECCCCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEE
KNHQVTLDNQKAPEEQMIGINNVNNQLNKGKGKLSFSMNGNQLKATSSNAGYGISYEDKN
CCCEEEECCCCCCHHHEEECCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6500704; 1587866; 8943190; 1400487; 8168947; 8188346; 7559447 [H]