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The map label for this gene is yhcA [H]

Identifier: 49185219

GI number: 49185219

Start: 2213994

End: 2215436

Strand: Direct

Name: yhcA [H]

Synonym: BAS2210

Alternate gene names: 49185219

Gene position: 2213994-2215436 (Clockwise)

Preceding gene: 49185218

Following gene: 49185220

Centisome position: 42.34

GC content: 36.38

Gene sequence:

>1443_bases
ATGAAGGCAAAAATAAATCCAAAAGTAGTTGTAAGCATTGTATATATAACAGCGATGTTTATGGCTGCTATGGATGCAAC
AATTGTAAATGTAGCACTACAAACGATAAGTAAAGAACTACAAGTTCCACCATCTGCGATGGGGACAGTTAATGTTGGGT
ATTTAGTTAGCTTAGCTGTCTTCCTTCCGATTTCTGGTTGGCTAGGAGATCGATTCGGTACGAAAAGGATATTTCTAACT
GCTCTTTTCGTTTTTACAACCGCATCTGCGTTATGTGGGATAGCAAACGATATTACTTCATTGAATATTTTTCGTATTAT
TCAAGGTGCTGGTGGGGGGCTTTTAACACCAGTTGGGATGGCGATGTTATTCCGAACATTTTCACCAGAGGAAAGGCCGA
AAATTTCTCGGTTTATTGTGTTGCCAATTGCTGTCGCACCGGCAGTAGGACCAATTATTGGGGGCTTTTTTGTAGATCAA
ATGTCTTGGCGCTGGGCATTTTATATTAATTTACCATTTGGAATTATGGCGTTACTATTCGGTCTGCTATTTTTAAAAGA
GCATATTGAAAAATCAGCTGGTCGCTTTGATTCTCTCGGTTTTATTCTATCAGCACCAGGATTTGCGATGATAATCTATG
CGCTTAGTCAAGGACCATCAAGAGGCTGGATTTCTACAGAGATTATAAGTACTGGAATAGCTGGGACTGTATTTATTACA
TTGTTTATTCTTGTAGAACTTAAAGTAAAGCAACCGATGTTAGATTTACGCTTATTAAAAGAACCAGTCTTTAGAAAAAT
GAGTCTTATTTCATTATTTTCATCAGCTGGTTTACTAGGAATGTTATTTGTTTTTCCTCTTATGTATCAAAATGTAATAG
GAGTTTCCGCGTTAGAATCAGGTCTAACGACATTTCCGGAGGCGATTGGTTTAATGATTTCTTCACAAATCGTACCATGG
TCATATAAGAAATTAGGAGCTCGTAAGGTAATTTCTATTGGATTAATTTGTACGGTGATTATCTTTGTTTTATTAAGTTT
TGTAAATCACGATACAAACCCTTGGCAAATACGGGCATTATTGTTTGGCATAGGTATTTTCTTAGGCCAGTCAGTTGGAG
CAGTTCAATTTTCTGCATTTAACAATATAACGCCGCCTTCTATGGGGAGAGCAACCACTATATTTAATGTGCAAAATCGA
TTAGGTTCAGCGATAGGAGTTGCTGTTTTAGCTAGTATACTAGCCGGTTTTGGAAATAACAGTGTACAACATAATGCGCA
TTCAGATTTCTTTCCATATCAAGCAGCATTAATTGGATCAGCAATATTTTTACTCGTAGCATTACTATTTTCTTTACGTC
TTTCTGATAAAGAGGTAATGTCAAAGAAGAAAGAAAAAATGTTATCCGTAACAAAAAAACAGAAAGCACTTGTCAATGAA
TAA

Upstream 100 bases:

>100_bases
CTAGTAGGAATGATTGTTTACAATATGTTGAAAATAACTGGGAAGATTTGAATCCAAAAAGTAATCAAGTTGGCAAAAAA
ATATTAGTAGGAAAACGATA

Downstream 100 bases:

>100_bases
CAATTGATATTCGTTATATAAAGGAGAGTTTACATGATAGAAAGTAAAGTTGTAGATTCTATACCAACTTTGAATGAGAA
TGATTGTCAAATATGGTGGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKAKINPKVVVSIVYITAMFMAAMDATIVNVALQTISKELQVPPSAMGTVNVGYLVSLAVFLPISGWLGDRFGTKRIFLT
ALFVFTTASALCGIANDITSLNIFRIIQGAGGGLLTPVGMAMLFRTFSPEERPKISRFIVLPIAVAPAVGPIIGGFFVDQ
MSWRWAFYINLPFGIMALLFGLLFLKEHIEKSAGRFDSLGFILSAPGFAMIIYALSQGPSRGWISTEIISTGIAGTVFIT
LFILVELKVKQPMLDLRLLKEPVFRKMSLISLFSSAGLLGMLFVFPLMYQNVIGVSALESGLTTFPEAIGLMISSQIVPW
SYKKLGARKVISIGLICTVIIFVLLSFVNHDTNPWQIRALLFGIGIFLGQSVGAVQFSAFNNITPPSMGRATTIFNVQNR
LGSAIGVAVLASILAGFGNNSVQHNAHSDFFPYQAALIGSAIFLLVALLFSLRLSDKEVMSKKKEKMLSVTKKQKALVNE

Sequences:

>Translated_480_residues
MKAKINPKVVVSIVYITAMFMAAMDATIVNVALQTISKELQVPPSAMGTVNVGYLVSLAVFLPISGWLGDRFGTKRIFLT
ALFVFTTASALCGIANDITSLNIFRIIQGAGGGLLTPVGMAMLFRTFSPEERPKISRFIVLPIAVAPAVGPIIGGFFVDQ
MSWRWAFYINLPFGIMALLFGLLFLKEHIEKSAGRFDSLGFILSAPGFAMIIYALSQGPSRGWISTEIISTGIAGTVFIT
LFILVELKVKQPMLDLRLLKEPVFRKMSLISLFSSAGLLGMLFVFPLMYQNVIGVSALESGLTTFPEAIGLMISSQIVPW
SYKKLGARKVISIGLICTVIIFVLLSFVNHDTNPWQIRALLFGIGIFLGQSVGAVQFSAFNNITPPSMGRATTIFNVQNR
LGSAIGVAVLASILAGFGNNSVQHNAHSDFFPYQAALIGSAIFLLVALLFSLRLSDKEVMSKKKEKMLSVTKKQKALVNE
>Mature_480_residues
MKAKINPKVVVSIVYITAMFMAAMDATIVNVALQTISKELQVPPSAMGTVNVGYLVSLAVFLPISGWLGDRFGTKRIFLT
ALFVFTTASALCGIANDITSLNIFRIIQGAGGGLLTPVGMAMLFRTFSPEERPKISRFIVLPIAVAPAVGPIIGGFFVDQ
MSWRWAFYINLPFGIMALLFGLLFLKEHIEKSAGRFDSLGFILSAPGFAMIIYALSQGPSRGWISTEIISTGIAGTVFIT
LFILVELKVKQPMLDLRLLKEPVFRKMSLISLFSSAGLLGMLFVFPLMYQNVIGVSALESGLTTFPEAIGLMISSQIVPW
SYKKLGARKVISIGLICTVIIFVLLSFVNHDTNPWQIRALLFGIGIFLGQSVGAVQFSAFNNITPPSMGRATTIFNVQNR
LGSAIGVAVLASILAGFGNNSVQHNAHSDFFPYQAALIGSAIFLLVALLFSLRLSDKEVMSKKKEKMLSVTKKQKALVNE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=465, Percent_Identity=30.5376344086021, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=28.5057471264368, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788392, Length=416, Percent_Identity=29.8076923076923, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=30.3614457831325, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788710, Length=340, Percent_Identity=30, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=476, Percent_Identity=23.3193277310924, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325455, Length=235, Percent_Identity=27.6595744680851, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319783, Length=390, Percent_Identity=23.5897435897436, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=262, Percent_Identity=27.0992366412214, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=313, Percent_Identity=23.961661341853, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=28.7581699346405, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52084; Mature: 52084

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKINPKVVVSIVYITAMFMAAMDATIVNVALQTISKELQVPPSAMGTVNVGYLVSLAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FLPISGWLGDRFGTKRIFLTALFVFTTASALCGIANDITSLNIFRIIQGAGGGLLTPVGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLFRTFSPEERPKISRFIVLPIAVAPAVGPIIGGFFVDQMSWRWAFYINLPFGIMALLF
HHHHHHCCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
GLLFLKEHIEKSAGRFDSLGFILSAPGFAMIIYALSQGPSRGWISTEIISTGIAGTVFIT
HHHHHHHHHHHHCCCHHCCCHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
LFILVELKVKQPMLDLRLLKEPVFRKMSLISLFSSAGLLGMLFVFPLMYQNVIGVSALES
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GLTTFPEAIGLMISSQIVPWSYKKLGARKVISIGLICTVIIFVLLSFVNHDTNPWQIRAL
HHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LFGIGIFLGQSVGAVQFSAFNNITPPSMGRATTIFNVQNRLGSAIGVAVLASILAGFGNN
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCC
SVQHNAHSDFFPYQAALIGSAIFLLVALLFSLRLSDKEVMSKKKEKMLSVTKKQKALVNE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAKINPKVVVSIVYITAMFMAAMDATIVNVALQTISKELQVPPSAMGTVNVGYLVSLAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FLPISGWLGDRFGTKRIFLTALFVFTTASALCGIANDITSLNIFRIIQGAGGGLLTPVGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AMLFRTFSPEERPKISRFIVLPIAVAPAVGPIIGGFFVDQMSWRWAFYINLPFGIMALLF
HHHHHHCCCCCCCCHHHEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
GLLFLKEHIEKSAGRFDSLGFILSAPGFAMIIYALSQGPSRGWISTEIISTGIAGTVFIT
HHHHHHHHHHHHCCCHHCCCHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHH
LFILVELKVKQPMLDLRLLKEPVFRKMSLISLFSSAGLLGMLFVFPLMYQNVIGVSALES
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
GLTTFPEAIGLMISSQIVPWSYKKLGARKVISIGLICTVIIFVLLSFVNHDTNPWQIRAL
HHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LFGIGIFLGQSVGAVQFSAFNNITPPSMGRATTIFNVQNRLGSAIGVAVLASILAGFGNN
HHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCC
SVQHNAHSDFFPYQAALIGSAIFLLVALLFSLRLSDKEVMSKKKEKMLSVTKKQKALVNE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]