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The map label for this gene is sbcD [H]

Identifier: 49185207

GI number: 49185207

Start: 2193271

End: 2194428

Strand: Direct

Name: sbcD [H]

Synonym: BAS2198

Alternate gene names: 49185207

Gene position: 2193271-2194428 (Clockwise)

Preceding gene: 49185206

Following gene: 49185208

Centisome position: 41.95

GC content: 35.84

Gene sequence:

>1158_bases
ATGAAGTTATTTCATACAGCGGATTGGCATTTAGGAAAGCTTGTTCATGGCGTGTATATGACTGAAGATCAAAAGATTGT
ATTAGATCAGTTTGTACAAGCAGTTGAAGAAGAAAAACCAGATGCCGTAATTATTGCAGGGGATTTATATGACCGAGCAA
TTCCACCTACGGAAGCAGTAGATTTATTAAATGATGTGCTGCAAAAAATAGTAATTGATTTACAAACACCAGTTATTGCA
GTTGCTGGTAACCATGATAGTCCAGATCGTATTCATTTTGGAAGTAGTTTAATGAAGAAACAAGGATTATTTATTGTGGG
GCAATTTCAATTTCCCTATGAACCAATTATATTAAATGATGAACATGGCGAGGTTCACTTCCATCTCGTTCCATATGCGG
ATCCGAGTATAGTTAGACATGTATTGAAAAACGAAGACGTACGTTCTCATGATGATGCGATGCGTATTTTTATGAATGAG
CTTTCTGAAACGATGGATAAAGAAGCTAGACATGTATTTGTTGGTCATGCATTTGTAACTTCTGCTGGAGAAGCGGAAGA
GAATACAAGTGATGCTGAACGACCGCTTTCAATTGGTGGTGCTGAATACGTAAATAGTCATTATTTTGATAAGTTTCATT
ACACAGCGCTTGGACATTTACATCAAGCGCATTTCGTACGTAATGAGACAATTCGATATTCAGGTTCGCCACTTGCCTAT
TCTATTTCTGAAGAAAAACATAAAAAAGGATATTATATTGTGGAACTGAACGAGCAAGGTGAAGCAACAATTGAAAAAAG
GTTACTTACACCACGTCGTAAAATGCGAACAGTAGAAGCGAAAATAGATGAATTATTACAACATCCAGTAAGTGAAGATT
ACGTGTTTGTAAAATTATTAGATGAAAATCCTGTCTTGCAGCCAATGGAAAAAATACGCTCTGTATATCCAAATGCAATG
CATGTTGAAAGATCCATTCAAAGACGGGAGTTTACAGAGTCAAATGAAGCAACGGTTTCAAGACATAAAACGGATGATTT
ATCACTATTAAAAGCTTTTTATAAAGAAATGAAAGGCTTAGATTTATCAGAAGAGAAAGAACGTTTATTTGTAGAAGTAT
TACAAACAGTGCAAGAACGGGAAGGTGAACGAGGATGA

Upstream 100 bases:

>100_bases
TAGTGCAATGATACAAAAGTTAGAATGGGAGAACGGAAAAACTACAATAAACTATCAACTTGTATCACTAAATTCAGTAA
ATTAAAAGGAGCAGGAAACC

Downstream 100 bases:

>100_bases
GACCGATCCAGCTTATTATGACGGCATTTGGCCCATATAAACAGAAAGAAGTAATTGATTTTGACGATCTGGGAGAGCAT
CGTATTTTCGCCATTTCTGG

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKLFHTADWHLGKLVHGVYMTEDQKIVLDQFVQAVEEEKPDAVIIAGDLYDRAIPPTEAVDLLNDVLQKIVIDLQTPVIA
VAGNHDSPDRIHFGSSLMKKQGLFIVGQFQFPYEPIILNDEHGEVHFHLVPYADPSIVRHVLKNEDVRSHDDAMRIFMNE
LSETMDKEARHVFVGHAFVTSAGEAEENTSDAERPLSIGGAEYVNSHYFDKFHYTALGHLHQAHFVRNETIRYSGSPLAY
SISEEKHKKGYYIVELNEQGEATIEKRLLTPRRKMRTVEAKIDELLQHPVSEDYVFVKLLDENPVLQPMEKIRSVYPNAM
HVERSIQRREFTESNEATVSRHKTDDLSLLKAFYKEMKGLDLSEEKERLFVEVLQTVQEREGERG

Sequences:

>Translated_385_residues
MKLFHTADWHLGKLVHGVYMTEDQKIVLDQFVQAVEEEKPDAVIIAGDLYDRAIPPTEAVDLLNDVLQKIVIDLQTPVIA
VAGNHDSPDRIHFGSSLMKKQGLFIVGQFQFPYEPIILNDEHGEVHFHLVPYADPSIVRHVLKNEDVRSHDDAMRIFMNE
LSETMDKEARHVFVGHAFVTSAGEAEENTSDAERPLSIGGAEYVNSHYFDKFHYTALGHLHQAHFVRNETIRYSGSPLAY
SISEEKHKKGYYIVELNEQGEATIEKRLLTPRRKMRTVEAKIDELLQHPVSEDYVFVKLLDENPVLQPMEKIRSVYPNAM
HVERSIQRREFTESNEATVSRHKTDDLSLLKAFYKEMKGLDLSEEKERLFVEVLQTVQEREGERG
>Mature_385_residues
MKLFHTADWHLGKLVHGVYMTEDQKIVLDQFVQAVEEEKPDAVIIAGDLYDRAIPPTEAVDLLNDVLQKIVIDLQTPVIA
VAGNHDSPDRIHFGSSLMKKQGLFIVGQFQFPYEPIILNDEHGEVHFHLVPYADPSIVRHVLKNEDVRSHDDAMRIFMNE
LSETMDKEARHVFVGHAFVTSAGEAEENTSDAERPLSIGGAEYVNSHYFDKFHYTALGHLHQAHFVRNETIRYSGSPLAY
SISEEKHKKGYYIVELNEQGEATIEKRLLTPRRKMRTVEAKIDELLQHPVSEDYVFVKLLDENPVLQPMEKIRSVYPNAM
HVERSIQRREFTESNEATVSRHKTDDLSLLKAFYKEMKGLDLSEEKERLFVEVLQTVQEREGERG

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=281, Percent_Identity=25.9786476868327, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: 3.1.11.-

Molecular weight: Translated: 44258; Mature: 44258

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFHTADWHLGKLVHGVYMTEDQKIVLDQFVQAVEEEKPDAVIIAGDLYDRAIPPTEAV
CCCEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHH
DLLNDVLQKIVIDLQTPVIAVAGNHDSPDRIHFGSSLMKKQGLFIVGQFQFPYEPIILND
HHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHHCCCEEEEEECCCCCCEEEEC
EHGEVHFHLVPYADPSIVRHVLKNEDVRSHDDAMRIFMNELSETMDKEARHVFVGHAFVT
CCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHEE
SAGEAEENTSDAERPLSIGGAEYVNSHYFDKFHYTALGHLHQAHFVRNETIRYSGSPLAY
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEE
SISEEKHKKGYYIVELNEQGEATIEKRLLTPRRKMRTVEAKIDELLQHPVSEDYVFVKLL
ECCHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DENPVLQPMEKIRSVYPNAMHVERSIQRREFTESNEATVSRHKTDDLSLLKAFYKEMKGL
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
DLSEEKERLFVEVLQTVQEREGERG
CCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLFHTADWHLGKLVHGVYMTEDQKIVLDQFVQAVEEEKPDAVIIAGDLYDRAIPPTEAV
CCCEECCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHH
DLLNDVLQKIVIDLQTPVIAVAGNHDSPDRIHFGSSLMKKQGLFIVGQFQFPYEPIILND
HHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHHCCCEEEEEECCCCCCEEEEC
EHGEVHFHLVPYADPSIVRHVLKNEDVRSHDDAMRIFMNELSETMDKEARHVFVGHAFVT
CCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHEE
SAGEAEENTSDAERPLSIGGAEYVNSHYFDKFHYTALGHLHQAHFVRNETIRYSGSPLAY
CCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEE
SISEEKHKKGYYIVELNEQGEATIEKRLLTPRRKMRTVEAKIDELLQHPVSEDYVFVKLL
ECCHHHCCCCEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DENPVLQPMEKIRSVYPNAMHVERSIQRREFTESNEATVSRHKTDDLSLLKAFYKEMKGL
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCC
DLSEEKERLFVEVLQTVQEREGERG
CCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA