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The map label for this gene is prsA

Identifier: 49185187

GI number: 49185187

Start: 2172298

End: 2173149

Strand: Direct

Name: prsA

Synonym: BAS2178

Alternate gene names: 49185187

Gene position: 2172298-2173149 (Clockwise)

Preceding gene: 49185185

Following gene: 49185188

Centisome position: 41.55

GC content: 32.28

Gene sequence:

>852_bases
TTGAAAAAGAAAAAGCTATTTTTAGGAACAATTATTTCATGCGTAGTACTGGCATTATCTGCATGTGGTTCCTCAGACAA
CGTAGTAACATCAAAAGTAGGAAATATTACAGAGAAAGAATTAAGTAAAGAATTAAGACAAAAATATGGGGAAAGTACTT
TATACCAAATGGTGTTAAGTAAGGCGTTGCTAGATAAATATAAAGTTTCAGATGAGGAAGCAAAAAAACAAGTAGAAGAA
GCAAAAGATAAAATGGGTGACAACTTCAAATCGACTTTAGAGCAAGTTGGATTGAAAAACGAAGATGAATTAAAAGAAAA
AATGAAGCCAGAAATTGCATTTGAGAAGGCGATTAAAGCAACTGTCACAGAAAAGGATGTAAAAGATAACTATAAACCAG
AAATGAAGGTAAGTCACATTTTAGTAAAAGATGAAAAGACTGCTAAAGAAGTGAAAGAGAAAGTGAATAATGGAGAAGAT
TTTGCAGCTTTAGCGAAGCAATACTCAGAGGATACTGGTTCAAAAGAACAGGGCGGAGAAATAACTGGTTTTGCTCCAGG
ACAAACGGTAAAAGAATTTGAGGAAGCTGCATATAAATTAGATGCAGGACAAGTAAGTGAGCCAGTAAAAACAACTTATG
GTTACCATATTATAAAAGTGACAGATAAAAAAGAATTGAAGCCATTTGATGAAGTAAAAGATTCAATTCGTAAAGATATA
GAACAGCAAAGACTGCAAGATACGACAGGTAAATGGAAACAGCAAGTAGTCAATGAATTATTGAAAGATGCCGATATTAA
GGTGAATGATAAAGAGTTTAAAAATACATTTGAATTTCTAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
GTTTTTTCAAATGAAATGAATTACATGTAGAAAACACATTGTTTACACAAGAACATTGTATAGTTATTCTTGTATAATAA
TAAAAGTGAGGTATTTTGAA

Downstream 100 bases:

>100_bases
TGACTGTAATTAGAAAAGGGAACCTTTAAAGGTTCCCTTTTCTAGTTAGTGTGTGAGTTATAAATAGATAGTAAGACAAT
ACAATATTACATATAAATAT

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILVKDEKTAKEVKEKVNNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPFDEVKDSIRKDI
EQQRLQDTTGKWKQQVVNELLKDADIKVNDKEFKNTFEFLEKK

Sequences:

>Translated_283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILVKDEKTAKEVKEKVNNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPFDEVKDSIRKDI
EQQRLQDTTGKWKQQVVNELLKDADIKVNDKEFKNTFEFLEKK
>Mature_283_residues
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLSKALLDKYKVSDEEAKKQVEE
AKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKATVTEKDVKDNYKPEMKVSHILVKDEKTAKEVKEKVNNGED
FAALAKQYSEDTGSKEQGGEITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPFDEVKDSIRKDI
EQQRLQDTTGKWKQQVVNELLKDADIKVNDKEFKNTFEFLEKK

Specific function: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Important for the secretion of the protective antigen. The three psrA proteins in this organism show different but overlapping

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain

Homologues:

Organism=Escherichia coli, GI1786238, Length=133, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790211, Length=82, Percent_Identity=41.4634146341463, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PRSA3_BACAN (Q81QT1)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_844720.1
- RefSeq:   YP_018981.2
- RefSeq:   YP_028439.1
- ProteinModelPortal:   Q81QT1
- SMR:   Q81QT1
- EnsemblBacteria:   EBBACT00000010056
- EnsemblBacteria:   EBBACT00000014383
- EnsemblBacteria:   EBBACT00000022935
- GeneID:   1084443
- GeneID:   2815680
- GeneID:   2849906
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_2336
- KEGG:   bar:GBAA_2336
- KEGG:   bat:BAS2178
- TIGR:   BA_2336
- TIGR:   GBAA_2336
- GeneTree:   EBGT00050000000187
- HOGENOM:   HBG712792
- OMA:   EISGFAP
- ProtClustDB:   PRK02998
- BioCyc:   BANT260799:BAS2178-MONOMER
- BioCyc:   BANT261594:GBAA2336-MONOMER
- BRENDA:   5.2.1.8
- HAMAP:   MF_01145
- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR008880
- Gene3D:   G3DSA:1.10.3120.10

Pfam domain/function: PF00639 Rotamase; SSF109998 Trigger_fac_C_bac

EC number: =5.2.1.8

Molecular weight: Translated: 32083; Mature: 32083

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01096 PPIC_PPIASE_1; PS50198 PPIC_PPIASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHH
KALLDKYKVSDEEAKKQVEEAKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
TVTEKDVKDNYKPEMKVSHILVKDEKTAKEVKEKVNNGEDFAALAKQYSEDTGSKEQGGE
HHHHHHHHCCCCCCHHHHHHEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
ITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPFDEVKDSIRKDI
EECCCCCHHHHHHHHHHHHCCCHHCCCCHHHHCCEEEEEECCCHHCCCHHHHHHHHHHHH
EQQRLQDTTGKWKQQVVNELLKDADIKVNDKEFKNTFEFLEKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKKLFLGTIISCVVLALSACGSSDNVVTSKVGNITEKELSKELRQKYGESTLYQMVLS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHH
KALLDKYKVSDEEAKKQVEEAKDKMGDNFKSTLEQVGLKNEDELKEKMKPEIAFEKAIKA
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHH
TVTEKDVKDNYKPEMKVSHILVKDEKTAKEVKEKVNNGEDFAALAKQYSEDTGSKEQGGE
HHHHHHHHCCCCCCHHHHHHEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
ITGFAPGQTVKEFEEAAYKLDAGQVSEPVKTTYGYHIIKVTDKKELKPFDEVKDSIRKDI
EECCCCCHHHHHHHHHHHHCCCHHCCCCHHHHCCEEEEEECCCHHCCCHHHHHHHHHHHH
EQQRLQDTTGKWKQQVVNELLKDADIKVNDKEFKNTFEFLEKK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12721629; 12606539