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The map label for this gene is alaXM [H]

Identifier: 49184612

GI number: 49184612

Start: 1618087

End: 1618797

Strand: Direct

Name: alaXM [H]

Synonym: BAS1597

Alternate gene names: 49184612

Gene position: 1618087-1618797 (Clockwise)

Preceding gene: 49184611

Following gene: 49184613

Centisome position: 30.95

GC content: 35.3

Gene sequence:

>711_bases
ATGACAACGGCATTATATTTAGAAGATGCATACAAAACGAGTTGCGAAACAGAAGTGATAAAAGTAGAAGGGAATAAAGT
ATTTGTAAAAGAAACAGTTTTTTATCCAACTGGTGGAGGGCAAGAATGTGATACGGGTGTCATTGTACAAGATGGGTTTG
TATTTGAAGTTGAAAAGGTAAAGAAGGAGCAGGGAGAAATAGTTCACTATATAAAAGATGGCGCTCAAGTAAAATTAGGT
CCCGTTAAATTAGAGATTAATTGGGAAAGACGTCATAATTTAATGCGTCATCACTCTTTACTGCATCTTATCGGAGCTGT
CGTTTATGAAAAATATGGAGCTCTATGCACTGGAAATCAAATTTATCCTGACAAAGCACGTATTGATTTTAATGAGTTAC
AAGAGTTATCGAGCGTGGAAGTAGAAGGAATTGTTAAGGAAGTGAATAAACTTATTGAACAAAATAAAGAAATTTCCACT
CGTTATATGAGCCGCGAAGAAGCAGAAAATGCTGTAGGAATGATTAAAACAGCAATAAATCTCCTGCCAACTACTATACA
AGAAATCCGCATTGTTACAATTGAAAATTTAGACGAACAAGCTTGTGGAGGCACACATGTGAAAAATACGAGTGAAATAG
GAACACTTGTTATTGATAAAGTAAAAAGTAAAGGGAAGCAAAATCGTCGCTTTGAAGTTAGAGCGATCTAG

Upstream 100 bases:

>100_bases
AGTCTTTTATTAATTTTAAATTTCAGAATTATCAATTGACTTTACGGAGCTTCTTTTTTATATTAGTAGGTATAACTATA
ACTACTAAAGAGGTGAGAGT

Downstream 100 bases:

>100_bases
ATTGCGAGGGGTTACCGATGGATGAAATTATAAAGGAATTACAAAAGTTAGGATTTTCCCAGTACGAATGTAAAGCGTAT
ATTGGACTACTAAAACATTC

Product: alanyl-tRNA synthetase domain-containing protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGVIVQDGFVFEVEKVKKEQGEIVHYIKDGAQVKLG
PVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEIST
RYMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI

Sequences:

>Translated_236_residues
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGVIVQDGFVFEVEKVKKEQGEIVHYIKDGAQVKLG
PVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEIST
RYMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
>Mature_235_residues
TTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGVIVQDGFVFEVEKVKKEQGEIVHYIKDGAQVKLGP
VKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTR
YMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=212, Percent_Identity=30.6603773584906, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI217416402, Length=212, Percent_Identity=30.6603773584906, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI109148542, Length=228, Percent_Identity=26.7543859649123, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789048, Length=212, Percent_Identity=28.3018867924528, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18543305, Length=216, Percent_Identity=27.3148148148148, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 26657; Mature: 26526

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGVIVQDGFVFEVEKV
CCEEEEEEHHHCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCCCCEEEECCEEEEHHHH
KKEQGEIVHYIKDGAQVKLGPVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQ
HHHCCCEEEEECCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCE
IYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTRYMSREEAENAVGMIKTAIN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
HHHHHHHHEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
TTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGVIVQDGFVFEVEKV
CEEEEEEHHHCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCCCCEEEECCEEEEHHHH
KKEQGEIVHYIKDGAQVKLGPVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQ
HHHCCCEEEEECCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCE
IYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTRYMSREEAENAVGMIKTAIN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
HHHHHHHHEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]