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The map label for this gene is rnjA [H]

Identifier: 49184537

GI number: 49184537

Start: 1552962

End: 1554635

Strand: Direct

Name: rnjA [H]

Synonym: BAS1522

Alternate gene names: 49184537

Gene position: 1552962-1554635 (Clockwise)

Preceding gene: 49184535

Following gene: 49184538

Centisome position: 29.7

GC content: 33.87

Gene sequence:

>1674_bases
TTGAAGTCATTAAAAAACAACTTGTCTATCGTTGCTTTAGGCGGAGTAAATGAAATAGGGAAAAATATGTATGCTATTCA
ATATGAAAATGATATTGTCGTTATTGATTGCGGATCTAAATTTCCAGATGAAAGTTTATTAGGAATTGATTTAATCATTC
CAGACGTCACATACTTACAAGAAAATAAAGAAAAAATTCGCGGATTAGTAGTAACGCATGGACATGAAGATCATATTGGC
GGAATACCATATTTTTTAAAACAACTAAACGTACCAATCTATGCGACGAGGTTAACGTTAGGTTTAATTGAAATTAAATT
AAAGGAACATAACCTTCAAAATGATACAGAATTAATCGTTATTCATTCAGAATCAGAAATTGATTTTGGTTCTATTAAGA
CGACTTTTTTTAAAACGAATCATAGCATTCCAGATTGCCTCGGTATTGCTTTCCATACACCAGAAGGTACTGTAGTACAT
ACAGGTGATTTTAAATTTGATTTAACACCAGTAAATAACCAACAGCCTGATATTCATAAAATGGCTAAAATAGGTAGTGA
AGGCGTGCTAGCTTTACTATCTGAAAGCACAAATGCAGAACGACCAGGATTTACTCCATCAGAAAGGTCGGTAGGGGAGC
GGATAGAAGAAATATTTATGAAAGCAAATAGGAAAGTGATTATTTCTACTTTTGCTTCTAATGTGAATCGTGTTCAGCAA
ATAGTTGAAGCTTGTATAAAAACAAATCGAAAACTGGCTTTGCTCGGGAGAAGTATGGTAAATGTAGTAGAAGTTGCTCT
AGAGAAAGGGTATCTAAATATTCCAGATGGTATGTTAATTGAAGCAAGTGAGGTAAATCGTTTAGATCCAAAGCAGGTAG
CAATCCTTTGTACAGGAAGCCAAGGAGAACCAATGGCAGCTTTAGCGCGGTTAGCTAGTGGAAACTATAGGCAAGTGGAT
GTTTTACCAGAAGATACGGTTATTATAGCGGCAACGCCTATTCCGGGAAATGAACGTAACGTTTCACGAATTATAGATAA
TTTATTCGCATTAGGAGCAAAAGTAATTTATGGATCAGGTAGTTCTACTGGAGTCCATGTATCTGGTCATGCGTATCAAG
AAGAATTGAAATTAATGCTTGCGTTAATGAAGCCAAAATATTTTATCCCTATTCATGGAGAATTTAGAATGCTACATCAT
CACAGTTTGTTAGCAGAATCAATAGGTGTTGAAAAAGAGAATATCTTTATCGTTCGTAATGGAGATGTTGTAGATATAAG
TAATGAAGTGGCAATTCAATCTAGAAAAATACAGGCAGGAAATATATATGTAGATGGATTAGGAATTGGTGATGTTGGAA
ATGCCTTATTACGTGATCGAAAACAACTTTCAGAAGATGGAATGCTTGTTATAGTGATTACTTTTAATAAAGCGGATGGA
GATATAATTTCAGGTCCGGATATTATATCTCGTGGATTTGTCTATGTTCGTGATTCAGAAGAATTTTTGAAGGAATTAAA
TAAATTAGCTGTTATTACGATTAATAATTTAAAGAAAGAGAAAGTGAATAGCTGGGGTATTTTGAAAAGAGAAGTAAGAG
AAGCTTTAGGGAAGTATATATATACGAATACGAAAAGAAAGCCGATGATTCTTCCTATAATAATAGAGGTGTAA

Upstream 100 bases:

>100_bases
TTTTATATGTATGAAATAGTATGAAGTCCTTTTCCATTACAAATAATAAAGCCTAAATACGATTTCACGAATTATAACTG
TGAAAGGGAGGGCATATAAT

Downstream 100 bases:

>100_bases
TAATGAATAAAAAGAATTCTCGTATGGGAATTCTTTTTTTGTTTTGAAGATAAAATGAACATATAATGAAGTAAATAGGA
ATGGAAGGGAATAAAAGATG

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Sequences:

>Translated_557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV
>Mature_557_residues
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLVVTHGHEDHIG
GIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELIVIHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVH
TGDFKFDLTPVNNQQPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
VLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHH
HSLLAESIGVEKENIFIVRNGDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61633; Mature: 61633

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVHTGDFKFDLTPVNNQQPDIHK
EECCCCCCCCCEEEEEEECCCCCCHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCEEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHHHSLLAESIGVEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC
>Mature Secondary Structure
MKSLKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLVVTHGHEDHIGGIPYFLKQLNVPIYATRLTLGLIEIKLKEHNLQNDTELIV
CCHHHHCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDFGSIKTTFFKTNHSIPDCLGIAFHTPEGTVVHTGDFKFDLTPVNNQQPDIHK
EECCCCCCCCCEEEEEEECCCCCCHHEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLNIPDGMLIEASEVNRLDPKQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCEEEEEEECC
QGEPMAALARLASGNYRQVDVLPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLALMKPKYFIPIHGEFRMLHHHSLLAESIGVEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRKIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKADG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
DIISGPDIISRGFVYVRDSEEFLKELNKLAVITINNLKKEKVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]