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The map label for this gene is hisH

Identifier: 49184336

GI number: 49184336

Start: 1352301

End: 1352930

Strand: Direct

Name: hisH

Synonym: BAS1319

Alternate gene names: 49184336

Gene position: 1352301-1352930 (Clockwise)

Preceding gene: 49184335

Following gene: 49184337

Centisome position: 25.86

GC content: 35.56

Gene sequence:

>630_bases
TTGATTGCCATTATAGATTATGGAATGGGAAATATTCGTAGTGTAGAACAAGCATTAAAATACATTGGAGCAGCGTACAT
CGTAACGAGTGATAAAGAAGAGATTTTTAGAAGTGATGGAGTGATTTTACCAGGAGTAGGTGCATTTCCAAAAGCTATGG
ATATATTGGAAGAAAAAGATTTAGTTCGTGTGTTACAAGAAATTGGGCGTTCAAGAAAACCACTTCTAGGCATTTGCTTA
GGAATGCAGCTTTTATTTGAAAAAAGTGAGGAACTCCAAGATTGTAACGGATTAAGTTTATTGCCAGGTGTTATTCGAAA
GTTAAAAGTTCCTTATAAAATTCCTCATATGGGATGGAATGAGTTAAAGAAAGAAGGAGAAATAGCGCTTTGGAATGGAG
TAGAGGACGGTTCTTTCGTATATTATGTCCACTCTTATTACGCAGATTGTCCAAATGAAATTGTGTATGGAATAAGTGAT
TATGGAGTGAAAGTACCTGGTTTTGTAGCAAAAGGAAATATATATGGTGCGCAGTTTCATCCTGAAAAAAGTGGTGACAT
TGGCATGCAAATGTTGAAAAATTTTAAAGGGGTGGTAGAAACATGGAAATCTTCCCAGCTATCGATTTAA

Upstream 100 bases:

>100_bases
AATTGAAGCATTATTTAAAGCGTTTGGTAGAGCGCTTAGAGAAGCAGTCGAAAGAAATGCCCACATTACTGGTGTAAATT
CAACGAAAGGGATGTTGTAA

Downstream 100 bases:

>100_bases
AAGAAGGGCGATGCGTGAGACTGTATCAAGGCGAGTTTAGTAAAGAAACAGTAATGAATGAGGACCCGGTTGCGCAAGCG
ATTATATTTGAAAAATTTGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRVLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI

Sequences:

>Translated_209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRVLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI
>Mature_209_residues
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKDLVRVLQEIGRSRKPLLGICL
GMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWNELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISD
YGVKVPGFVAKGNIYGAQFHPEKSGDIGMQMLKNFKGVVETWKSSQLSI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=39.5, Blast_Score=134, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BACAA (C3P504)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002865920.1
- ProteinModelPortal:   C3P504
- SMR:   C3P504
- EnsemblBacteria:   EBBACT00000130329
- GeneID:   7849009
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1495
- GeneTree:   EBGT00050000000583
- ProtClustDB:   PRK13141
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23286; Mature: 23286

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 180-180 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKD
CEEEEECCCCCHHHHHHHHHHHCEEEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LVRVLQEIGRSRKPLLGICLGMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHH
ELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISDYGVKVPGFVAKGNIYGAQFH
HHHCCCCEEEECCCCCCCEEEEEEHHHHCCCHHHEECHHHCCEECCCEEECCCEEECEEC
PEKSGDIGMQMLKNFKGVVETWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIAIIDYGMGNIRSVEQALKYIGAAYIVTSDKEEIFRSDGVILPGVGAFPKAMDILEEKD
CEEEEECCCCCHHHHHHHHHHHCEEEEEECCHHHHHHCCCEEECCCCCCHHHHHHHHHHH
LVRVLQEIGRSRKPLLGICLGMQLLFEKSEELQDCNGLSLLPGVIRKLKVPYKIPHMGWN
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCHH
ELKKEGEIALWNGVEDGSFVYYVHSYYADCPNEIVYGISDYGVKVPGFVAKGNIYGAQFH
HHHCCCCEEEECCCCCCCEEEEEEHHHHCCCHHHEECHHHCCEECCCEEECCCEEECEEC
PEKSGDIGMQMLKNFKGVVETWKSSQLSI
CCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA