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The map label for this gene is nrdE [H]

Identifier: 49184286

GI number: 49184286

Start: 1306788

End: 1308110

Strand: Direct

Name: nrdE [H]

Synonym: BAS1269

Alternate gene names: 49184286

Gene position: 1306788-1308110 (Clockwise)

Preceding gene: 49184285

Following gene: 49184287

Centisome position: 24.99

GC content: 34.77

Gene sequence:

>1323_bases
TTGACCAAAAAAATAAATGTCGATGAGAAGAGCCGTATTCAGTCTCTATCAATCGGAATTATCGTTCCAAGTAAGTTCTT
TGAGCTTGCTGAGAAAAACGAACCTTTCCATGTTTTCGCGCCTTATACTGTATATAAAGAATACGGAAAGCATTTAGATG
ACATTGATATCGATGAAATGTACGATGAATTAATGAGCAATCCAAAAGTGAAGAAGAAGCCGCTAGATATTAGTGCGCGT
GATATGCTTATTAAAATTGCTATGATTCAGCTTGAGTCTGGTTATCCGTACTTAATGTTTAAATCAAATGCAAATAACCA
ACATCCATTGAAGGACATTGGAACTGTGAAGATGTCCAACTTGTGTACAGAAATCTTCCAGCTACAAGAAACTTCTGAAA
TTAACGATTACGGTACAGATGACATTATTCGTCGTGATATTAACTGTAACTTAGGATCGTTAAATATCGTAAATGTAATG
GAAAATAAAGAAATTCGTGAAGCAGTTCATGCGGGAATGGAAGCTTTAACAGCTGTTTCTGATATGACGATCATTCCGAA
TGCACCAACTGTGAAAAAAGCAAATGATGAGCTTCATTCAGTTGGGCTTGGCGCAATGAACTTACACGGATATTTAGCAA
AAAATAAAATCGCTTATGAAAGTGAAGAAGCGAAAGAGTTCGCTCGTACATTCTTTATGATGTTAAATTACTACTCTATT
GAGAAAAGTATGGAAATCGCTAAAGAAAAAGGCGAAACATTTAAAGACTTCGATAAATCTGATTATGCGAATGGGACATA
CTTTGAAAAGTATGAAACGACAGATTACAGCCCAGTAACTGAAAAAGTTCAGCAATTATTTGAAGGAATTCATATTCCAA
CAAAAGAAGATTGGACAAGTTTAAAAGAGCAAGTGCAGAAGAATGGTTTATACAACTCATATCGTCTTGCTATCGCTCCG
ACACAATCAATCAGTTACGTTCAAAATGCAACTTCAAGCGTAATGCCTATCGTAAGTCAAATCGAATCAAGAACGTATGC
GAATGCGACAACATATTATCCAATGCCGTATTTATCAAAAGATACGTTCTGGTACTATAAATCTTCTTACGATATGAATC
AGTTTAAATTAATTGATTTAATCGCAGAAATTCAAGAACATATTGACCAAGGAATTAGTACAATTCTTTACGTTAATAGT
GATATTTCAACACGTGAATTAGCACGTTACTACATCTATGCACATAAAAAAGGCTTAAAGAGTCTGTATTATACGAGAAC
ACGTAAATTAAGCGTGGAAGAGTGTGTGGCTTGTACTGTTTAA

Upstream 100 bases:

>100_bases
AGCTAGTAGAGTAGGCTGGAAATAGGTACCAGTCGAAGTGCAAGGCAACCAGTTACAGGTTGATGATATAGTCCAACCTT
CATAGAAATATGAAGACGAG

Downstream 100 bases:

>100_bases
TATAAAAAGTGAGACATGATGAGCACGGTTTTCTGAACCCTGCTCATGCTCAGTTATGAATTTTTCATGTTTATTTAGAG
AAGGGGCGATTGAATGCGTG

Product: ribonucleoside-diphosphate reductase subunit alpha, group I intron-containing

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTKKINVDEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISAR
DMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVM
ENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESEEAKEFARTFFMMLNYYSI
EKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAP
TQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNS
DISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Sequences:

>Translated_440_residues
MTKKINVDEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISAR
DMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVM
ENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESEEAKEFARTFFMMLNYYSI
EKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAP
TQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNS
DISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
>Mature_439_residues
TKKINVDEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARD
MLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVME
NKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESEEAKEFARTFFMMLNYYSIE
KSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPT
QSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSD
ISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=468, Percent_Identity=24.7863247863248, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1789030, Length=447, Percent_Identity=40.9395973154362, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI2367133, Length=434, Percent_Identity=22.5806451612903, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17555242, Length=462, Percent_Identity=24.025974025974, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322123, Length=474, Percent_Identity=23.8396624472574, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320914, Length=474, Percent_Identity=23.4177215189873, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17352481, Length=468, Percent_Identity=24.5726495726496, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 50648; Mature: 50517

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKINVDEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEM
CCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHH
YDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSN
HHHHHCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
LCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTAVS
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
DMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESEEAKEFARTFFMMLNYYSI
CCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTS
HHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
LKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSK
HHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
DTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKGLK
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SLYYTRTRKLSVEECVACTV
HHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
TKKINVDEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEM
CCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCHHHH
YDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSN
HHHHHCCCCCCCCCCCCCHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
LCTEIFQLQETSEINDYGTDDIIRRDINCNLGSLNIVNVMENKEIREAVHAGMEALTAVS
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
DMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESEEAKEFARTFFMMLNYYSI
CCEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EKSMEIAKEKGETFKDFDKSDYANGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTS
HHHHHHHHHCCCHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
LKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSK
HHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
DTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVNSDISTRELARYYIYAHKKGLK
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
SLYYTRTRKLSVEECVACTV
HHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]