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The map label for this gene is nrdE [H]

Identifier: 49184284

GI number: 49184284

Start: 1304924

End: 1305691

Strand: Direct

Name: nrdE [H]

Synonym: BAS1267

Alternate gene names: 49184284

Gene position: 1304924-1305691 (Clockwise)

Preceding gene: 49184283

Following gene: 49184285

Centisome position: 24.96

GC content: 37.76

Gene sequence:

>768_bases
TTGCGACACATTGAACTGAATAATGAAATCACGCAAATGCAGGACGGTTTTTATCAGCTTCATAAAGATAAAGAGGCATT
AGAAGTCTTTATGGAAGAAGCTAGAGAGAATACCGTTCATTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAAC
ATGATTACTATTACAACGTTCTGGACGAGTATAACTTGGAGGAAGTAGAAGAGGTATATAACATCGCTTATGGTGAAAAC
TTCGAGTTCCAATCTTATATGGCAGCATCTAAGTTTTACAAAGATTATGCGTTAAAAACAAATGATCAAAAACAATACTT
AGAAAGCTATGAAGATCGTGTAGCAATTGTGTCATTATACTTAGGACGCGGTGATGTTGCCAAGGCAAAACAATTCGCAA
GCATGATTGTCAAACAAAACTACCAACCAGCAACACCAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTG
TCTTGTTTCTTGTTAGAGATGGATGATAGCTTAAATTCAATCGGCTTTAACATTAATACTGCAATGCAATTATCAAAAAT
CGGCGGAGGAGTAGCTTTAAACTTATCTAAGCTACGCGCACGTGGTGAGCAAATTAAAGGTATCGACAACGCTGCAAGTG
GTGTAGTACCTGTTATGAAGTTACTTGAAGATTCGTTTTCATATGCGAATCAGCTTGGCCAACGAAAAGGTGCTGGCGCA
GTATACTTGAACATTTTCCATTGGGATATTATTGAATTCCTGGATTAA

Upstream 100 bases:

>100_bases
GACAAAATTTCTGCTAAATATGAAGTGCCTATTGTATCAAAATTTGAGTTATCAGGAACAAATAACGATGTAGAATATTT
TAAGGAAAGGGTGCGGGAGA

Downstream 100 bases:

>100_bases
TTGGTAGTCCCTCTACACAGTAATGTGTATGAGAAAACCTTGTGAACCTAACCAATAGGGTGTCGCATTAGCGGCTAACG
GTATCAGTTGAATAAGGGGT

Product: ribonucleoside-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLD

Sequences:

>Translated_255_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLD
>Mature_255_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=183, Percent_Identity=32.2404371584699, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789030, Length=242, Percent_Identity=49.5867768595041, Blast_Score=241, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17555242, Length=182, Percent_Identity=31.3186813186813, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322123, Length=269, Percent_Identity=25.2788104089219, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320914, Length=183, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17352481, Length=203, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 29350; Mature: 29350

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLD
EEEEEHHHHHHHHCC
>Mature Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLD
EEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]