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The map label for this gene is odhB [H]

Identifier: 49184194

GI number: 49184194

Start: 1223181

End: 1224437

Strand: Reverse

Name: odhB [H]

Synonym: BAS1176

Alternate gene names: 49184194

Gene position: 1224437-1223181 (Counterclockwise)

Preceding gene: 49184195

Following gene: 49184193

Centisome position: 23.42

GC content: 41.21

Gene sequence:

>1257_bases
ATGATCGAAATTAAAGTACCTGAGCTTGCAGAATCTATTACTGAAGGAACTATCTCACAATGGCTTCTCAACGTAGGCGA
CAAAGTTGAGAAAGGTGGCAGCGTTGTTGAGCTTGAAACTGATAAAGTCAATGTAGAAATCATTGCAGAAGATTCAGGTA
TTGTATCGAAGTTACTAGGCGAACCTGGGGATACAGTTGAAGTTGGCGCAACTATCGCAATTTTAGACGCAAACGGAGCA
CCAGTTGCAGTAAGTACACCTGCACCGGCTGAGCAATCGAAACAAGAAACAGCTGAAGCACCAAAAGCTGCGGCACCAAG
TGCTGAACAAACTGCAACTCTACAAGGTTTACCAAATACAAACCGTCCTATCGCATCACCAGCTGCTAGAAAAATGGCTC
GTGAATTAGGAATCGACTTAAACGACGTACGTAGCACAGATCCACTTGGCCGTGTGAGACCACACGATGTACAAGCTCAT
GCTGCAGCACCAAAAGAAGCACCAGCTGCTCCAAAAAGTCCAGCTCCTGCTCCAGTTGCAAAAACTGAATTCGAAAAACC
AGTTGAGCGCGTGAAAATGTCCCGCCGCCGTCAAACAATTGCAAAACGTCTTGTAGAAGTTCAACAAACATCTGCAATGT
TAACAACATTTAACGAAGTTGATATGACTGCAATCATGGAATTACGTAAAGAGCGTAAAGATGCTTTCGAGAAAAAACAT
GATGTACGTCTTGGTTTCATGTCATTCTTCACAAAAGCAGTTGTTGCAGCATTAAAACAATTCCCATTATTAAATGCTGA
AATTCAAGGCGACGAGCTTATCATTAAAAAATTCTATGATATCGGTATTGCAGTAGCAGCTCCAGATGGATTAGTTGTTC
CAGTTGTACGCGATGCGAACCAATTAAACTTCGCTGAAATCGAAAGCGAAATTCGTGAATTAGGTAAAAAAGCACGTGAT
AACAAACTTTCATTAAAAGAACTACAAGGTGGTACATTTACAATTACAAACGGTGGTGTGTTCGGTTCTCTAATGTCAAC
ACCGATCCTAAACAGCCCACAAGTTGGTATTTTAGGAATGCACAAAATCCAAGTACGTCCAGTTGCAATCGATAACGAAC
GTATGGAAAACCGCCCAATGATGTACATCGCTTTATCTTACGATCACCGTATTGTTGATGGTAAAGAAGCAGTTAGCTTC
CTTGTTGCTGTTAAAGATATGCTTGAAGATCCAAAATCATTATTATTAGAAGGTTGA

Upstream 100 bases:

>100_bases
GCAGATATTCTGCCCAAGGTCAGGCTAAAACAAATGGAGATTACCGAAGACAAAAGAAGAAAAAACACACCGTTAGGCAA
ATAAGGGGAGGACATTTAAA

Downstream 100 bases:

>100_bases
TAGATTCATAATCTATTACAGGCTGTAGAATATATCATTCTACAGCTTTTTTTATTGCATTCATACGGGACAAATCGCAT
GACATAAGGTAATAATGTAT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIEIKVPELAESITEGTISQWLLNVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAEQSKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAH
AAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAIMELRKERKDAFEKKH
DVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKARD
NKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSF
LVAVKDMLEDPKSLLLEG

Sequences:

>Translated_418_residues
MIEIKVPELAESITEGTISQWLLNVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAEQSKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAH
AAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAIMELRKERKDAFEKKH
DVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKARD
NKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSF
LVAVKDMLEDPKSLLLEG
>Mature_418_residues
MIEIKVPELAESITEGTISQWLLNVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAEQSKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAH
AAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAIMELRKERKDAFEKKH
DVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKARD
NKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSF
LVAVKDMLEDPKSLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=56.7099567099567, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=30.4347826086957, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=29.2307692307692, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=29.2307692307692, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=27.4599542334096, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=416, Percent_Identity=45.1923076923077, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=33.5680751173709, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=39.9049881235154, Blast_Score=277, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=29.7921478060046, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=29.1571753986333, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=265, Percent_Identity=31.6981132075472, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=43.5096153846154, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=29.8901098901099, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=53.8116591928251, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=26.9320843091335, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=32.3404255319149, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=32.3404255319149, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45261; Mature: 45261

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKVPELAESITEGTISQWLLNVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLG
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCEEEEEEEECCCHHHHHHHC
EPGDTVEVGATIAILDANGAPVAVSTPAPAEQSKQETAEAPKAAAPSAEQTATLQGLPNT
CCCCEEEECEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCHHCEEECCCCCC
NRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAHAAAPKEAPAAPKSPAPAPVA
CCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
KTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAIMELRKERKDAFEKKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDAN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCC
QLNFAEIESEIRELGKKARDNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGM
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCEEEE
HKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSFLVAVKDMLEDPKSLLLEG
EEEEEEEEEECCCHHCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MIEIKVPELAESITEGTISQWLLNVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLG
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCEEEEEEEECCCHHHHHHHC
EPGDTVEVGATIAILDANGAPVAVSTPAPAEQSKQETAEAPKAAAPSAEQTATLQGLPNT
CCCCEEEECEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCHHCEEECCCCCC
NRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAHAAAPKEAPAAPKSPAPAPVA
CCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
KTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMTAIMELRKERKDAFEKKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDAN
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCCC
QLNFAEIESEIRELGKKARDNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGM
CCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCEEEE
HKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSFLVAVKDMLEDPKSLLLEG
EEEEEEEEEECCCHHCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]