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The map label for this gene is spsI [H]

Identifier: 49184155

GI number: 49184155

Start: 1187110

End: 1187847

Strand: Direct

Name: spsI [H]

Synonym: BAS1135

Alternate gene names: 49184155

Gene position: 1187110-1187847 (Clockwise)

Preceding gene: 49184154

Following gene: 49184156

Centisome position: 22.7

GC content: 36.99

Gene sequence:

>738_bases
GTGAAAGGGATTATTTTAGCAGGTGGTACTGGATCGAGATTATATCCAATAACGAAAGTAACGAATAAACATTTACTTCC
TGTTGGTCGGTATCCGATGATTTATCATGCGGTATATAAGTTAAAACAATGTGATATTACAGATATTATGATTATTACAG
GTAAAGAGCATATGGGGGATGTTGTTAGCTTTTTAGGGAGCGGTCAAGAGTTTGGCGTGTCCTTTACGTATCGTGTGCAA
GATAAAGCTGGCGGAATTGCACAAGCATTAGGGCTTTGTGAAGATTTTGTTGGGAATGATCGCATGGTAGTTATATTAGG
TGATAATATTTTTTCAGATGATATTCGTCCGTATGTTGAAGAGTTTACAAATCAAAAAGAAGGTGCGAAAGTACTGCTGC
AATCTGTAGATGATCCGGAGAGATTTGGCGTAGCAAATATTCAAAACCGCAAAATAATTGAAATTGAAGAAAAGCCGAAA
GAGCCGAAAAGTTCCTATGCAGTTACAGGAATTTACTTGTATGATTCGAAAGTCTTTTCTTATATAAAAGAATTAAAACC
TTCCGCAAGGGGAGAACTTGAAATTACAGATATCAATAATTGGTATTTAAAGCGAGGGGTACTTACTTATAATGAAATGA
GCGGTTGGTGGACTGATGCGGGAACTCATGTTTCTCTTCAAAGAGCGAATGCGTTAGCACGGGATATAAACTTTGGTAAA
CAGTTTAACGGAGAATAG

Upstream 100 bases:

>100_bases
ACGGGGATGAGTTTGATGCGGATACATATGAGAAGTACCGGAAAGTGTTTGTAGAACATTACAAAGATATATTATCTCCA
TCATAAAGGAGAGATGCAAA

Downstream 100 bases:

>100_bases
GTGAGAATATGAAAGTTATAGAAACAAACTTTACCGACGCAAAATTGTTGGAACCGAGGTTATTTGGAGATGATCGTGGC
TTTTTTACTGAAAGTTATAA

Product: glucose-1-phosphate thymidylyltransferase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCDITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFTNQKEGAKVLLQSVDDPERFGVANIQNRKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKRGVLTYNEMSGWWTDAGTHVSLQRANALARDINFGK
QFNGE

Sequences:

>Translated_245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCDITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFTNQKEGAKVLLQSVDDPERFGVANIQNRKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKRGVLTYNEMSGWWTDAGTHVSLQRANALARDINFGK
QFNGE
>Mature_245_residues
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCDITDIMIITGKEHMGDVVSFLGSGQEFGVSFTYRVQ
DKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVEEFTNQKEGAKVLLQSVDDPERFGVANIQNRKIIEIEEKPK
EPKSSYAVTGIYLYDSKVFSYIKELKPSARGELEITDINNWYLKRGVLTYNEMSGWWTDAGTHVSLQRANALARDINFGK
QFNGE

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI11761619, Length=227, Percent_Identity=26.8722466960352, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788351, Length=233, Percent_Identity=47.6394849785408, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790224, Length=242, Percent_Identity=46.6942148760331, Blast_Score=201, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI133931050, Length=224, Percent_Identity=24.5535714285714, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=226, Percent_Identity=29.2035398230088, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 27502; Mature: 27502

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCDITDIMIITGKEHMGD
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHH
VVSFLGSGQEFGVSFTYRVQDKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVE
HHHHHCCCHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
EFTNQKEGAKVLLQSVDDPERFGVANIQNRKIIEIEEKPKEPKSSYAVTGIYLYDSKVFS
HHCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECHHHHH
YIKELKPSARGELEITDINNWYLKRGVLTYNEMSGWWTDAGTHVSLQRANALARDINFGK
HHHHHCCCCCCCEEEEECCCEEEECCEEEEECCCCEECCCCCEEEHHHHHHHHHHCCCCC
QFNGE
CCCCC
>Mature Secondary Structure
MKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKLKQCDITDIMIITGKEHMGD
CCCEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHH
VVSFLGSGQEFGVSFTYRVQDKAGGIAQALGLCEDFVGNDRMVVILGDNIFSDDIRPYVE
HHHHHCCCHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
EFTNQKEGAKVLLQSVDDPERFGVANIQNRKIIEIEEKPKEPKSSYAVTGIYLYDSKVFS
HHCCCCHHHHHHHHCCCCHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECHHHHH
YIKELKPSARGELEITDINNWYLKRGVLTYNEMSGWWTDAGTHVSLQRANALARDINFGK
HHHHHCCCCCCCEEEEECCCEEEECCEEEEECCCCEECCCCCEEEHHHHHHHHHHCCCCC
QFNGE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]