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The map label for this gene is azoR1

Identifier: 49183931

GI number: 49183931

Start: 969927

End: 970589

Strand: Reverse

Name: azoR1

Synonym: BAS0908

Alternate gene names: 49183931

Gene position: 970589-969927 (Counterclockwise)

Preceding gene: 49183934

Following gene: 49183929

Centisome position: 18.56

GC content: 31.83

Gene sequence:

>663_bases
ATGAACAAAACACTTATCATAAACGCACATCCGAAAGTGGATGATACATCATCAGTAAGCATTAAGGTTTTTAAGCATTT
TTTAGAATCTTATAAAGAATTAATTTCTAATAATGAAACGATTGAACAGATTAATTTATACGATGATGTAGTACCAATGA
TAGATAAAACTGTTTTAAGCGCATGGGAAAAACAAGGTAATGGACAAGAGTTGACTCGTGAAGAACAAAAAGTAACGGAA
CGTATGTCCGAGATTTTACAACAATTTAAAAGTGCAAATACGTATGTTATCGTATTACCTCTGCATAATTTTAATATTCC
ATCAAAGTTAAAAGATTACATGGACAATATCATGATTGCACGTGAAACATTTAAATATACTGAAACTGGATCAGTGGGAC
TACTTAAAGATGGAAGAAGAATGCTCGTTATACAAGCAAGTGGAGGAATCTATACAAATGATGATTGGTATACAGACGTG
GAATACTCTCATAAATATTTAAAAGCAATGTTTAATTTCCTTGGTATTGAAGATTATCAAATCGTTCGTGCACAAGGAAC
AGCAGTACTAGATCCAACTGAAGTATTACAAAACGCATATAAAGAAGTTGAAGAGGCTGCTTCTAGATTGGCTAACAAAT
ATATTTTTTCACTGGAAGAATGA

Upstream 100 bases:

>100_bases
GACAAAAATAATGTCAGAGTTTATATTAAAGACCAAGTTAATCGACGATTAACTTGGTCGACAATTAAAGCATAAAAACT
TTTAAAAAGGGGAAATACAA

Downstream 100 bases:

>100_bases
TGGTTATAAATAAAGATCCCCTCCACCTTAAGGCAGAGGGGACTTTCCAATTCCTATTTCAAACTACCCGCAATCTCCGT
AAAGATAACCCCAAGCGCAT

Product: hypothetical protein

Products: 4'- Phosphopantetheine; Apoprotein. [C]

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNETIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVLPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDDWYTDV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANKYIFSLEE

Sequences:

>Translated_220_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNETIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVLPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDDWYTDV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANKYIFSLEE
>Mature_220_residues
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNETIEQINLYDDVVPMIDKTVLSAWEKQGNGQELTREEQKVTE
RMSEILQQFKSANTYVIVLPLHNFNIPSKLKDYMDNIMIARETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDDWYTDV
EYSHKYLKAMFNFLGIEDYQIVRAQGTAVLDPTEVLQNAYKEVEEAASRLANKYIFSLEE

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=163, Percent_Identity=33.1288343558282, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR1_BACAN (Q81UB2)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017225
- EMBL:   AE017334
- RefSeq:   NP_843471.1
- RefSeq:   YP_017598.1
- RefSeq:   YP_027183.1
- ProteinModelPortal:   Q81UB2
- EnsemblBacteria:   EBBACT00000009586
- EnsemblBacteria:   EBBACT00000015517
- EnsemblBacteria:   EBBACT00000023616
- GeneID:   1088173
- GeneID:   2817676
- GeneID:   2850159
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0966
- KEGG:   bar:GBAA_0966
- KEGG:   bat:BAS0908
- TIGR:   BA_0966
- TIGR:   GBAA_0966
- GeneTree:   EBGT00070000032324
- HOGENOM:   HBG565834
- OMA:   PAPLKEY
- ProtClustDB:   CLSK2394535
- BioCyc:   BANT260799:BAS0908-MONOMER
- BioCyc:   BANT261594:GBAA0966-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14 [C]

Molecular weight: Translated: 25369; Mature: 25369

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNETIEQINLYDDVVPMIDKTVLS
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AWEKQGNGQELTREEQKVTERMSEILQQFKSANTYVIVLPLHNFNIPSKLKDYMDNIMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
RETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDDWYTDVEYSHKYLKAMFNFLGIEDYQ
HHHHCCCCCCCEEEEECCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCE
IVRAQGTAVLDPTEVLQNAYKEVEEAASRLANKYIFSLEE
EEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNKTLIINAHPKVDDTSSVSIKVFKHFLESYKELISNNETIEQINLYDDVVPMIDKTVLS
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AWEKQGNGQELTREEQKVTERMSEILQQFKSANTYVIVLPLHNFNIPSKLKDYMDNIMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
RETFKYTETGSVGLLKDGRRMLVIQASGGIYTNDDWYTDVEYSHKYLKAMFNFLGIEDYQ
HHHHCCCCCCCEEEEECCCEEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCE
IVRAQGTAVLDPTEVLQNAYKEVEEAASRLANKYIFSLEE
EEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [Acyl-Carrier Protein]; H2O [C]

Specific reaction: [Acyl-Carrier Protein] + H2O = 4'- Phosphopantetheine + Apoprotein. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629