Click here to switch to the map view.

The map label for this gene is lmrB [H]

Identifier: 49183911

GI number: 49183911

Start: 951257

End: 952717

Strand: Direct

Name: lmrB [H]

Synonym: BAS0888

Alternate gene names: 49183911

Gene position: 951257-952717 (Clockwise)

Preceding gene: 49183909

Following gene: 49183915

Centisome position: 18.19

GC content: 36.82

Gene sequence:

>1461_bases
ATGAATATGACAACTAAGAGCCCTGCAAGTGGCAAAGTTGATACTTCTAATATGAAACATACCCCCATTTTAATCGCATT
ACTTTTAGGGACAATGGTTGCATTATTAAACGAAACGTTACTTGGTAATGCATTAACAGTATTAATGAAAGAATTTGACG
TAACAGCTTCAACGATTCAATGGCTTTCTACAGCTTACATGTTAGTAGTTGGGGTTTTAGTCCCAATTACAGCGTTACTT
CAGCAGTGGCTTACAACGAGACAGATGTTTTTGATCGCTATGGTAACATTTTTAGTCGGTACATTAATTGCGGGATTCGC
ACCGACATTTTCTGTTTTATTAGTAGGCCGTATCGTCCAAGCTGTAGCGACGGGCTTAATTTCACCGTTATTAATGAATA
CAATTTTAATTATTTGTCCACCTGAAAAACGTGGTGCCACAATGGGATTAATCGCACTTGTGATGATGTCCGCTCCAGCA
ATTGGTCCTACATTATCTGGAGTAATTGTGGATTCACTCAATTGGCGCTGGTTATTCTATATTGTAATTCCTATTGTAAT
CATTTCAATTATGATTGGGATGAAGTACATTCAAAATGTTTCAGAGATAACAAGGCCAAAAGTAGATTATCCATCTATCC
TTTTATCGACATTAGGATTTGGCGGGCTTGTGTATAGCTTTAGTGCATCAGGTGATTTAGGGTGGTCTGATACGAAAGTG
TATGGTGCTTTAATTGTTGGGCTTATCTCATTATGTATTTTTATCGTGCGACAATTAAAAATTGATAATCCAATTTTAGA
ATTACGAGCATTTAAAGTTCCGATGTTTACATTATCAGTCGGATTAATAGTCATTGTGATGATGTCGTTATTTTCAACGA
TGACTTTATTACCGATGTTCCTACAAACAGTTTTACTCGTTACAGCTTTTAAATCAGGAATCATCATGCTTCCAGGAAGT
GTTATTAGCGCCATAATGGGACCAATCGCAGGTAAACTATTTGATAAATTTAGTCCGAAAGTTATTATCGTTCCCGGCAT
TGTGTTAGTAGGAATTGCAATGTTCTTATTTAAAGGCATTACTCCTGATACATCAATAGTACAAATTATTGTGATGCATA
GCGTATTAATGGTTGGACTCATGTTTGTCATGACAGCACAGACATACGGCTTAAATCAATTAACACCAGATTTATATCCG
CACGGAACAGCGTTGTTTAATACTCTGCAGCAAGTAGCTGGCGCAATTGGTACAGCTATCTTTATTTCGAAAATGTCTTC
AGGAACAACTAGCTATATGGAAAGCTCCGCAAATCCAATGGACCCAGTAGAGAAGTTAAACGGCTTAACATCTGGGTTCC
AAGGTGCATTTGCACTTGGATTAGTCTTTATCGTTGTTGCATTTATCGTATCGTTGTTCTTAAAAGAAGAGAAAAAGGGA
GTTTTACAGAACGAAAATTAA

Upstream 100 bases:

>100_bases
AAATTGATTCTCCCTTTTTAATCTACATATGTATATTATATTACATGTGTTTGGGTTTATATAAAGCTTTTTGATTAAGA
AATAGGAGGAGAATCTGCAA

Downstream 100 bases:

>100_bases
GGTTTATATAAAAAGACATCTTTCAGAGGGAAAGATGTCTTTTACATTTCCTCCATAACATCATACCGAAGCAACTCTTC
CTGATCATAAAACCTCTCAA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGTMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSEITRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YGALIVGLISLCIFIVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLVFIVVAFIVSLFLKEEKKG
VLQNEN

Sequences:

>Translated_486_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGTMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSEITRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YGALIVGLISLCIFIVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLVFIVVAFIVSLFLKEEKKG
VLQNEN
>Mature_486_residues
MNMTTKSPASGKVDTSNMKHTPILIALLLGTMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGVLVPITALL
QQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPA
IGPTLSGVIVDSLNWRWLFYIVIPIVIISIMIGMKYIQNVSEITRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
YGALIVGLISLCIFIVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKSGIIMLPGS
VISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGITPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYP
HGTALFNTLQQVAGAIGTAIFISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLVFIVVAFIVSLFLKEEKKG
VLQNEN

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=25.5813953488372, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=333, Percent_Identity=24.9249249249249, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=24.1299303944316, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1790195, Length=390, Percent_Identity=24.8717948717949, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788392, Length=314, Percent_Identity=25.796178343949, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=317, Percent_Identity=25.5520504731861, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=135, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=320, Percent_Identity=23.125, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52307; Mature: 52307

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTKSPASGKVDTSNMKHTPILIALLLGTMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPIVIISIMIGMKYIQNVSEITRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YGALIVGLISLCIFIVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLVFIVVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLQNEN
CCCCCC
>Mature Secondary Structure
MNMTTKSPASGKVDTSNMKHTPILIALLLGTMVALLNETLLGNALTVLMKEFDVTASTIQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WLSTAYMLVVGVLVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVATGLISPLLMNTILIICPPEKRGATMGLIALVMMSAPAIGPTLSGVIVDSLNWRWLFY
HHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHH
IVIPIVIISIMIGMKYIQNVSEITRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDTKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCHHHH
YGALIVGLISLCIFIVRQLKIDNPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQTVLLVTAFKSGIIMLPGSVISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKGI
HHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCC
TPDTSIVQIIVMHSVLMVGLMFVMTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FISKMSSGTTSYMESSANPMDPVEKLNGLTSGFQGAFALGLVFIVVAFIVSLFLKEEKKG
HHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VLQNEN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]