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The map label for this gene is tenA [H]

Identifier: 49183715

GI number: 49183715

Start: 752355

End: 753050

Strand: Direct

Name: tenA [H]

Synonym: BAS0691

Alternate gene names: 49183715

Gene position: 752355-753050 (Clockwise)

Preceding gene: 49183712

Following gene: 49183716

Centisome position: 14.39

GC content: 35.34

Gene sequence:

>696_bases
ATGAAATTTTGCGATAGATTATTAGAAACTGTACAACCAGTTTGGGAGATGAGTCATAATCATCCGTTTGTAGTAGGTAT
GGGGGATGGTACGTTAGAAAAAGATAAGTTTCAGTATTATATTATTCAAGATTATTTATATCTGCTAGATTATGCAAAGC
TATATGCTATTGGCGTTGTAAAGGCAACGAATCCACAAGTAATGGGGAAATTTGCTGAACAAATTGATGGAATATTAAAT
GGAGAAATGACAATCCATAAACAGTATGCAAAAAGACTTGGAATTTCTATAGAGGAGATAGAGTCTGCAAAGCCATCTGC
TAAAAATTTAGCTTATACAAATTACATGATGTCTGTATCTCAAAATGGCACACTTGCAGAATTAATAGCAGCACTTCTTC
CGTGTATGTGGAGCTACTGGGAAATTGGAAAGCGTTTAAATGATATTCCTGGAGCAAGGGATCATGAATTTTTTGGTGAG
TGGATTCAAGGATATAGTTCTGAAGAGTACGGCAACCTCTGTATTTGGTTAATCGATTTATTAAATGAAATGGCAGTTGG
AAAGTCTGAAAAAGAGCTAGACCGATTAGAGGAGATTTTCCTATATTCCAGTCGATTTGAATATTTATTCTGGGATATGT
CCTATCGTAAGGAGATGTGGGGATTTGAGGAGCAAGAACATACTACAGTTTCATAA

Upstream 100 bases:

>100_bases
GTGGAACAACGAATAAATTATAATTTTATTTGCTGTAACCACTTCTTATATAAAGAAGTGGTTTTTTATTTATATACACA
TACCGGAAGGGGATAGAGAA

Downstream 100 bases:

>100_bases
TGTTTCTTTTCATTATGATGAGAAGCCAATCATCAATGAATTAAATGCTTCTATACAAGATAAAGAGTTTGTAAGTATTA
TCGGACCGAGTGGTTGCGGA

Product: transcriptional activator TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Sequences:

>Translated_231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
>Mature_231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=206, Percent_Identity=32.0388349514563, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=30.2439024390244, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325378, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26964; Mature: 26964

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]