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The map label for this gene is amaA [H]

Identifier: 49183697

GI number: 49183697

Start: 724379

End: 725554

Strand: Direct

Name: amaA [H]

Synonym: BAS0672

Alternate gene names: 49183697

Gene position: 724379-725554 (Clockwise)

Preceding gene: 49183696

Following gene: 49183698

Centisome position: 13.85

GC content: 39.8

Gene sequence:

>1176_bases
ATGGATGTTGCAAAAGAACTTGTTTTGTCAAAGGATCAGTTGGTTGAGTGGAGAAGGCATTTTCATAAGTATCCAGAGTT
ATCTTTTCAAGAAGAAAAAACATCACAGTTTGTATTCGACATACTTCGGAAAATCCCATGTTTAGAAGTGTCAAGACCTA
CTAAATATAGTGTAATGGCAAGGTTGATTGGAAAGCAGTCTGGTAAAACTATTGCGGTTCGAGCTGACATGGATGCTCTT
CCTATTCATGAAGAAAATGAGTTTGACTTTATTTCTGCATATCCAGGTGTAATGCATGCATGTGGCCATGATGGACATAT
AGCGATATTACTTGGAGTCGTACATAAGTTAGTAGAAGCAAGAGAGAAGATTAAAGGAGAGGTTCGTTTTCTATTCCAGC
ATGCTGAAGAAAACTTTCCTGGCGGAGCAGAGGAAATGGTCGCGGCTGGTGTAATGGAAGGGGTCGATTATATTGTTGGT
GCTCACCTTTGGGCGTCATTAGAGGTTGGGAAAGTAGGTGTAATTTATGGTCCTGCAATGGCTGCCCCAGATGTTTTTAA
AATTACGATAGAAGGAAAAGGTGGACATGCTGGAATCCCTCATGAAACGGTTGATAGTATTGCCATCGGCACACAAGTCG
TTTCACAACTTCAGCAAATTGTATCTCGTCTCACGAATCCGTTAGATTCTCTCGTAGTATCTGTTACGCAATTTCATGCT
GGGACAACCCATAATGTAATTCCAGCACAAACGGAGATTGAAGGGACAGTGCGGAGTTTAAGACATGAGTTACGAGAAGA
AACAGAGAAAAGGATTGAACAGATTGTAAAGCATGTGACGGAAGCTTATGGAGCGAAATATACTTTTTCTTATGAATATG
GATATCGTCCAGTAGTAAATGATTATGAAGTGACAGAGATTATTGAGCAAACAGCATTACAGCTTTATGGAAGGGAACGA
GTTACTCGTTTACAGCCGACGATGGCTGGAGAAGATTTTTCAGCGTTTTTACAAAAAGTACCAGGGACATTCTTTTTTAT
CGGAGCAGGAAGTAAAGAGAAAGGAATTATATATCCTCATCATCACCCTCGTTTTACGATTGATGAAGATGCATTACCAA
TTGGCGTGCAAGTCTTTGTATCATCGATTATGAATTTCATAAGTAAAGGAGAATGA

Upstream 100 bases:

>100_bases
ATTTTTAATCATTTATAATAGAAAATTCAGAAGATTCTTTTAAAAGTTAACAATTCACGTTATAATAACATTTAACTTGG
TGAAGAAGGGGAGAAGAGAA

Downstream 100 bases:

>100_bases
GATGAAGAAAATACACGTATTAGCGCTTATTCCAGTTTTTTGTTTAGTTGTTGGCCCCGTATTTGCTAATTCAGTTACTC
CTTACATATTAGGGATGCCA

Product: N-acyl-L-amino acid amidohydrolase

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKIPCLEVSRPTKYSVMARLIGKQSGKTIAVRADMDAL
PIHEENEFDFISAYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEVRFLFQHAEENFPGGAEEMVAAGVMEGVDYIVG
AHLWASLEVGKVGVIYGPAMAAPDVFKITIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPAQTEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKVPGTFFFIGAGSKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE

Sequences:

>Translated_391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKIPCLEVSRPTKYSVMARLIGKQSGKTIAVRADMDAL
PIHEENEFDFISAYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEVRFLFQHAEENFPGGAEEMVAAGVMEGVDYIVG
AHLWASLEVGKVGVIYGPAMAAPDVFKITIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPAQTEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKVPGTFFFIGAGSKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE
>Mature_391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKIPCLEVSRPTKYSVMARLIGKQSGKTIAVRADMDAL
PIHEENEFDFISAYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEVRFLFQHAEENFPGGAEEMVAAGVMEGVDYIVG
AHLWASLEVGKVGVIYGPAMAAPDVFKITIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPAQTEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKVPGTFFFIGAGSKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=424, Percent_Identity=24.7641509433962, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43673; Mature: 43673

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKIPCLEVSRPTKYSVMA
CCHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
RLIGKQSGKTIAVRADMDALPIHEENEFDFISAYPGVMHACGHDGHIAILLGVVHKLVEA
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHH
REKIKGEVRFLFQHAEENFPGGAEEMVAAGVMEGVDYIVGAHLWASLEVGKVGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCC
AAPDVFKITIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPAQTEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEVTEIIEQTALQLYGRERVTRLQPTMAGEDFSAFLQKVPGTFFFIGAGSKEKGIIYPH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVQVFVSSIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILRKIPCLEVSRPTKYSVMA
CCHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
RLIGKQSGKTIAVRADMDALPIHEENEFDFISAYPGVMHACGHDGHIAILLGVVHKLVEA
HHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHH
REKIKGEVRFLFQHAEENFPGGAEEMVAAGVMEGVDYIVGAHLWASLEVGKVGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCC
AAPDVFKITIEGKGGHAGIPHETVDSIAIGTQVVSQLQQIVSRLTNPLDSLVVSVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPAQTEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEVTEIIEQTALQLYGRERVTRLQPTMAGEDFSAFLQKVPGTFFFIGAGSKEKGIIYPH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVQVFVSSIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]