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The map label for this gene is ywkD [H]

Identifier: 49183599

GI number: 49183599

Start: 621221

End: 621607

Strand: Direct

Name: ywkD [H]

Synonym: BAS0574

Alternate gene names: 49183599

Gene position: 621221-621607 (Clockwise)

Preceding gene: 49183595

Following gene: 49183600

Centisome position: 11.88

GC content: 36.43

Gene sequence:

>387_bases
ATGAATATAAGTAGAGTGCATCACGTTGCAATTATTTGTTCGAATTATGAAGTGTCAAAGGATTTTTATACTAGGATATT
GGGTTTTAAAGCGATAAATGAAGTATATAGAAAGGAAAGAGATTCTTATAAGTTAGATTTATGTGTAGGAAAAGAGTATC
AAATTGAATTATTTTCATTTCCGAATCCGCCGAAGCGCCCGAGCTTCCCAGAAGCGGCTGGACTTAGGCATTTAGCATTT
GCTGTTACAAATATAGAAGAAGCCGTGCAAGATTTAAGTCAATGTGGCGTTGAAACTGAGGCGATACGTATTGATGAGAT
AACCGGGAAAAAATTTGTTTTTTTCCAAGACCCTGACGGTCTACCGTTAGAATTGTATGAGGTTTGA

Upstream 100 bases:

>100_bases
AAATTTCATAGTAAGCCCCCTAAAAGAGATATTCTGCTATATATGTTTTTCATTTCAAGGGAGAATATGCATCTTTGGTA
AAGAAAGGAATAAAGAAGTC

Downstream 100 bases:

>100_bases
AATTGGTGAATTTTATATAGGGTTGCTTTCGAGTAAATGAAGAAACTAAAGTGAAACTTCTTTTTAAAAGGAGGTGTAAC
TTTAGCTAAGAAAGCTAAAG

Product: glyoxylase family protein

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNISRVHHVAIICSNYEVSKDFYTRILGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAF
AVTNIEEAVQDLSQCGVETEAIRIDEITGKKFVFFQDPDGLPLELYEV

Sequences:

>Translated_128_residues
MNISRVHHVAIICSNYEVSKDFYTRILGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAF
AVTNIEEAVQDLSQCGVETEAIRIDEITGKKFVFFQDPDGLPLELYEV
>Mature_128_residues
MNISRVHHVAIICSNYEVSKDFYTRILGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAF
AVTNIEEAVQDLSQCGVETEAIRIDEITGKKFVFFQDPDGLPLELYEV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081697, Length=127, Percent_Identity=59.0551181102362, Blast_Score=156, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 14719; Mature: 14719

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISRVHHVAIICSNYEVSKDFYTRILGFKAINEVYRKERDSYKLDLCVGKEYQIELFSF
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PNPPKRPSFPEAAGLRHLAFAVTNIEEAVQDLSQCGVETEAIRIDEITGKKFVFFQDPDG
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCC
LPLELYEV
CCEEEEEC
>Mature Secondary Structure
MNISRVHHVAIICSNYEVSKDFYTRILGFKAINEVYRKERDSYKLDLCVGKEYQIELFSF
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEC
PNPPKRPSFPEAAGLRHLAFAVTNIEEAVQDLSQCGVETEAIRIDEITGKKFVFFQDPDG
CCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCC
LPLELYEV
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]