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The map label for this gene is pphA [H]

Identifier: 49183565

GI number: 49183565

Start: 577815

End: 578519

Strand: Direct

Name: pphA [H]

Synonym: BAS0539

Alternate gene names: 49183565

Gene position: 577815-578519 (Clockwise)

Preceding gene: 49183564

Following gene: 49183566

Centisome position: 11.05

GC content: 35.04

Gene sequence:

>705_bases
ATGAAAAGAATTCTTGTTATTAGTGATATTCATGGGGAAATAGAAAAGTTCGAACAGTTATTAGAAGAAGCTCAATACGA
TGCAAAACAGGATCAACTAATTTTATTAGGGGATTATGTAGATCGTGGTCCAAATGCGCGTGCAGTAATTGAAAAAGTAA
AAGAGTTAAAAGAAGATGGAGCCCTCGTTCTAAAGGGCAATCATGAAGATATGATGATCAAGGCGTTAACGACTGATGAG
GAACGTTCATGGAACCATTGGGTAAAAAGGAATGGTGGAGATAAGACATTATACAGCTATGGATTTGTAGAAGAAGATAT
TATGGTTAATGAGAAGGACTTCCGAAAACCGATTTTACAATCTCTTGTATTAGAGGAACATGTAAAGTTTATTCAAAAAT
TAGACCATTACATTGAAACAGAAGAGTATATATTTGTACATGCTGGTGTTGAGCCATTGAAACGAGTTTCTGAATGTGAA
CCATATACGTTAATGTGGATCCGTAATGAGTTTCATAGTGAGTATAGTGGTGAAAAGGTTGTTGTATTTGGCCATACGGA
AACAAAAACGCTTCATGGAAATGATAATTGTAATGTCTATTTTGGAAATAACCGTATTATAGGAATTGATGGTGGTGCTG
TATATGGTGGCCAGCTAAATTGTTTAGAATTGCCGAGTAAAAAGGTGTACGTAGTAAAAAGTTGA

Upstream 100 bases:

>100_bases
GCTTTGTTTGTAATAAAAGTGATTCTGAATATGATTTCGGAAATTTCTGTCGTTTTGAACTATAATTATAAAGAGAGTGG
TTTAGAAAGAAGGGGATAAT

Downstream 100 bases:

>100_bases
AAGAGATAAGGAGAATCTTATTTCTTTCAACTTTTTATTAAGCAAAATTTAAGGAAGAGTTTTTATAATAAGTCTTTCAA
ATTTTATATCAAAAAAACGT

Product: serine/threonine phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIMVNEKDFRKPILQSLVLEEHVKFIQKLDHYIETEEYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHSEYSGEKVVVFGHTETKTLHGNDNCNVYFGNNRIIGIDGGAVYGGQLNCLELPSKKVYVVKS

Sequences:

>Translated_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIMVNEKDFRKPILQSLVLEEHVKFIQKLDHYIETEEYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHSEYSGEKVVVFGHTETKTLHGNDNCNVYFGNNRIIGIDGGAVYGGQLNCLELPSKKVYVVKS
>Mature_234_residues
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVKELKEDGALVLKGNHEDMMIKALTTDE
ERSWNHWVKRNGGDKTLYSYGFVEEDIMVNEKDFRKPILQSLVLEEHVKFIQKLDHYIETEEYIFVHAGVEPLKRVSECE
PYTLMWIRNEFHSEYSGEKVVVFGHTETKTLHGNDNCNVYFGNNRIIGIDGGAVYGGQLNCLELPSKKVYVVKS

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 27024; Mature: 27024

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVKELKEDG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALVLKGNHEDMMIKALTTDEERSWNHWVKRNGGDKTLYSYGFVEEDIMVNEKDFRKPILQ
EEEEECCCCCEEEEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHH
SLVLEEHVKFIQKLDHYIETEEYIFVHAGVEPLKRVSECEPYTLMWIRNEFHSEYSGEKV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCEEEEEEEHHHHHCCCCCEE
VVFGHTETKTLHGNDNCNVYFGNNRIIGIDGGAVYGGQLNCLELPSKKVYVVKS
EEEECCCCEEEECCCCEEEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MKRILVISDIHGEIEKFEQLLEEAQYDAKQDQLILLGDYVDRGPNARAVIEKVKELKEDG
CCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
ALVLKGNHEDMMIKALTTDEERSWNHWVKRNGGDKTLYSYGFVEEDIMVNEKDFRKPILQ
EEEEECCCCCEEEEEECCCCCCCCHHHHHCCCCCCEEEECCCCCCCEEECCHHHHHHHHH
SLVLEEHVKFIQKLDHYIETEEYIFVHAGVEPLKRVSECEPYTLMWIRNEFHSEYSGEKV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCEEEEEEEHHHHHCCCCCEE
VVFGHTETKTLHGNDNCNVYFGNNRIIGIDGGAVYGGQLNCLELPSKKVYVVKS
EEEECCCCEEEECCCCEEEEECCCEEEEECCCEEECCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]