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The map label for this gene is aroF [H]

Identifier: 46447292

GI number: 46447292

Start: 1996454

End: 1997545

Strand: Direct

Name: aroF [H]

Synonym: pc1658

Alternate gene names: 46447292

Gene position: 1996454-1997545 (Clockwise)

Preceding gene: 46447288

Following gene: 46447293

Centisome position: 82.69

GC content: 36.81

Gene sequence:

>1092_bases
ATGGAACTTCTCCCCTCATATAAAGAATTAAAAAGCCAATTTCCGGCAAGCGCATCTCAAGATAATTTCATTGAAGATTG
TCGAGAAACTGTAAAATCTATTTTAAATGGTACTGATAAGCGATTGTTATTGATTGTAGGGCCCTGCTCAATCCATGATT
TTCATTCTGCTAAGGAATTTGCATTTAGATTAAAATGCTTAGCAGAATCTCTTTCGTCTCATTTTTTCATTTTAATGCGT
GTCTATTGTGAAAAAGCGAGAACAGCAACGGGCTGGAAAGGATTTTTGTATGATCCACTTTTAGACGGTTCCAATCAACT
TTTACTCGGAATCAAAAAGACAAGAGAATTGTTGCTAGAGCTTTGCGATTTGCGAATCCCTGCCGCAACCGAATTTTTAG
ATCCTTTAACCGCTTATTATTATGAAGATTTAATTTCTTGGGGGTCGATTGGAGCGAGAACTTCCTCTTCACAAATCCAC
AGGCAATTTGCTTCGAATTTGCAAATTCCGATAGGCTTTAAAAATGGAATTGCTGGTAATATTTCAGCTGCTGTTCAAGG
AGCCTATGCGGCTTCTCAACCCCATGCTTACATGGCTTTACACGAAAATGGATGGCCAATTGCAACGCAATCTAAAGGAA
ATCTCGATACTCACATTGTTTTAAGAGGTGGGGAAACAAAAACCAATTTTGACTCTCATTCGATATCTGAAGCGCTTGCA
AGGTTATCCCATGCAAACTTGCCAGAAAGAGTGTTGATCGATTGTTCTCATCATAATTCAAATAAAAAACATGAAAAACA
ACCAATTGTTTTTCAATCCATTATCGATCAAGTACTGGCAGGAAATTCCTTTATTAAAGGTTTAATGATAGAAAGTCACC
TTTATGCCGGCAATCAACCTTTTCATTTCAATCCACAAGAGCTTCGTTATGGTGTTTCGATTACGGATTCTTGCTTAGAT
TGGAATTCAACTCAAACACTCATTGAATGGGGTGCTCATCAATTGCAAGAAAATCTTAATCCGAGTAAGAAGTTATCTTT
GTCTTCAGAATCTCTTTTTAGTTCGATGATATGTACAAAGCTAAAAAGTTAA

Upstream 100 bases:

>100_bases
TTGGTAATTTTGAAAAAATAAACCCAAAACTTCAGTTAGATTGATTCACGAAAATGTCAATCTTTGTTAGACTCCGGACT
AATCAGAATAATGGACTAAC

Downstream 100 bases:

>100_bases
AAAACGCTTTAATAAAAAGCTCCTTAAAAATCTTAATAGCTATCATCTGCTTTCTTTGTCCGACTAGCTGTAGCCTTCAA
CGATTGAACGTACAAACTCA

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MELLPSYKELKSQFPASASQDNFIEDCRETVKSILNGTDKRLLLIVGPCSIHDFHSAKEFAFRLKCLAESLSSHFFILMR
VYCEKARTATGWKGFLYDPLLDGSNQLLLGIKKTRELLLELCDLRIPAATEFLDPLTAYYYEDLISWGSIGARTSSSQIH
RQFASNLQIPIGFKNGIAGNISAAVQGAYAASQPHAYMALHENGWPIATQSKGNLDTHIVLRGGETKTNFDSHSISEALA
RLSHANLPERVLIDCSHHNSNKKHEKQPIVFQSIIDQVLAGNSFIKGLMIESHLYAGNQPFHFNPQELRYGVSITDSCLD
WNSTQTLIEWGAHQLQENLNPSKKLSLSSESLFSSMICTKLKS

Sequences:

>Translated_363_residues
MELLPSYKELKSQFPASASQDNFIEDCRETVKSILNGTDKRLLLIVGPCSIHDFHSAKEFAFRLKCLAESLSSHFFILMR
VYCEKARTATGWKGFLYDPLLDGSNQLLLGIKKTRELLLELCDLRIPAATEFLDPLTAYYYEDLISWGSIGARTSSSQIH
RQFASNLQIPIGFKNGIAGNISAAVQGAYAASQPHAYMALHENGWPIATQSKGNLDTHIVLRGGETKTNFDSHSISEALA
RLSHANLPERVLIDCSHHNSNKKHEKQPIVFQSIIDQVLAGNSFIKGLMIESHLYAGNQPFHFNPQELRYGVSITDSCLD
WNSTQTLIEWGAHQLQENLNPSKKLSLSSESLFSSMICTKLKS
>Mature_363_residues
MELLPSYKELKSQFPASASQDNFIEDCRETVKSILNGTDKRLLLIVGPCSIHDFHSAKEFAFRLKCLAESLSSHFFILMR
VYCEKARTATGWKGFLYDPLLDGSNQLLLGIKKTRELLLELCDLRIPAATEFLDPLTAYYYEDLISWGSIGARTSSSQIH
RQFASNLQIPIGFKNGIAGNISAAVQGAYAASQPHAYMALHENGWPIATQSKGNLDTHIVLRGGETKTNFDSHSISEALA
RLSHANLPERVLIDCSHHNSNKKHEKQPIVFQSIIDQVLAGNSFIKGLMIESHLYAGNQPFHFNPQELRYGVSITDSCLD
WNSTQTLIEWGAHQLQENLNPSKKLSLSSESLFSSMICTKLKS

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=339, Percent_Identity=42.1828908554572, Blast_Score=306, Evalue=9e-85,
Organism=Escherichia coli, GI1786969, Length=335, Percent_Identity=43.8805970149254, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1787996, Length=329, Percent_Identity=44.0729483282675, Blast_Score=282, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320240, Length=341, Percent_Identity=39.8826979472141, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6319726, Length=345, Percent_Identity=39.4202898550725, Blast_Score=269, Evalue=5e-73,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 40518; Mature: 40518

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLPSYKELKSQFPASASQDNFIEDCRETVKSILNGTDKRLLLIVGPCSIHDFHSAKEF
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
AFRLKCLAESLSSHFFILMRVYCEKARTATGWKGFLYDPLLDGSNQLLLGIKKTRELLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEHHHHHHHHHH
LCDLRIPAATEFLDPLTAYYYEDLISWGSIGARTSSSQIHRQFASNLQIPIGFKNGIAGN
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
ISAAVQGAYAASQPHAYMALHENGWPIATQSKGNLDTHIVLRGGETKTNFDSHSISEALA
HHHHHHCCHHCCCCCEEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RLSHANLPERVLIDCSHHNSNKKHEKQPIVFQSIIDQVLAGNSFIKGLMIESHLYAGNQP
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
FHFNPQELRYGVSITDSCLDWNSTQTLIEWGAHQLQENLNPSKKLSLSSESLFSSMICTK
CCCCHHHHEECCCEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHH
LKS
HCC
>Mature Secondary Structure
MELLPSYKELKSQFPASASQDNFIEDCRETVKSILNGTDKRLLLIVGPCSIHDFHSAKEF
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
AFRLKCLAESLSSHFFILMRVYCEKARTATGWKGFLYDPLLDGSNQLLLGIKKTRELLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCEEEEHHHHHHHHHH
LCDLRIPAATEFLDPLTAYYYEDLISWGSIGARTSSSQIHRQFASNLQIPIGFKNGIAGN
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCC
ISAAVQGAYAASQPHAYMALHENGWPIATQSKGNLDTHIVLRGGETKTNFDSHSISEALA
HHHHHHCCHHCCCCCEEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
RLSHANLPERVLIDCSHHNSNKKHEKQPIVFQSIIDQVLAGNSFIKGLMIESHLYAGNQP
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
FHFNPQELRYGVSITDSCLDWNSTQTLIEWGAHQLQENLNPSKKLSLSSESLFSSMICTK
CCCCHHHHEECCCEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHH
LKS
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]