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The map label for this gene is folC [H]

Identifier: 46447260

GI number: 46447260

Start: 1945299

End: 1946531

Strand: Direct

Name: folC [H]

Synonym: pc1626

Alternate gene names: 46447260

Gene position: 1945299-1946531 (Clockwise)

Preceding gene: 46447259

Following gene: 46447261

Centisome position: 80.57

GC content: 36.17

Gene sequence:

>1233_bases
ATGCCTTATTCAGAATTGATTCAACGTCTGTTCGCGCTTAATCTTCATGGAGGAATAAAATTAGGTTTATCAAATTGTGA
AAAATTGCAAACAATCCTTAAGTTTCCTGACCATTCTTTTTCATCTATTCACGTGGCAGGAACAAATGGCAAAGGATCGG
TGGTTGCAAAAATCGCATCAGCACTTGAATATGCTGGCTATCGCGTCGGAGTCTATACGTCCCCTCACATTTCCTCCTTT
CGTGAGCGAATTCGCGTTAATGGAATAATGATTTCTGAGGCAGATGTTGAAAAAATTCTCTCCCAACTTTTTAATTTGAC
TGCTCAATATTCTATTCCTGCGACTTTTTTTGAACTGACTACTTTTTTAGCACTTGTCTATTTTTCTCGAGAAAAAGTTG
ATTTTGCCGTTTTAGAGGCGGGTTTAGGTGGGCGTTTAGATGCAACAAACATTGTCTCTCCCGTTCTTTCAATCATTACT
TCTATTAGTCTAGACCATACAGATATTTTAGGTGATACCCTTGAAGCAATTGCTAAAGAAAAAGCTGGTATCATTAAATC
GCACACTCCTGTCATTATTGGCCCACAAACACCTTACCTATTTATTGAAAAGCTAGCTTATCAAAAAAATAGCTTCTGCC
ATACTATAAAAAATCAATTTCTACTTTTCGATCAAGAAAACAATGCAATTGCCAAAAAATCCCTAGAAGTTTTAAGTGAA
AACTGGGATATCCCTCAAGAGGCTATCTTTAAAGGGTTAGAAGCTAAACAACCCTGCCGTTTCGAAATTATAAATAATTC
GGTAATTTTGGACGTCGCACATAATCCAAGCGGCCTTACTTCACTGTTTGCAAAAACTCAAACTCACTTTCCAAATCAAC
CTTTGAGAATTTTATTTGGATTATCAAAAGGTAAAGATTTAGAGGGTTGTTTGAAAATTTTAAGTCGCCATGGAAGTGCT
TTTCATCTTGTCGAAGCTCCTAATGGCAGAGGGATTTCATTTGATCTGTTAAAGCAAGGCCTTATGAATACAGGAATTGC
GTCTACAGTCATTTATTCTCATATAAATATTCAAGAAGGAGTAAAAATAGCAATAAAGCAAGCAGAGTCCAGCGGCCAAC
TATTACTTATTTGTGGTTCATTTTTTATTATGAGCCAAGTTAGGCAAGCCTTAGGTATGATAGAACACTTGGACTCGATA
GAACTTAATGAAAAAAGCATGAATTCATATTAA

Upstream 100 bases:

>100_bases
GCAATCAAACCAGATCTCCCTTTGGGATGTTCATGGGTTAATCAAGACACTTTTTCCATTTTAGAAAATCACTATAAAAA
CAATTGCCATACCTAACATT

Downstream 100 bases:

>100_bases
TTTATCAATTTGATAATTTAAAAAAATAAAGATGACTATTCCTAAATTTTTCAAATAATAGTCACTTTTTTTGAATAACT
CAAATTTTAGATTTATCAAC

Product: putative folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPYSELIQRLFALNLHGGIKLGLSNCEKLQTILKFPDHSFSSIHVAGTNGKGSVVAKIASALEYAGYRVGVYTSPHISSF
RERIRVNGIMISEADVEKILSQLFNLTAQYSIPATFFELTTFLALVYFSREKVDFAVLEAGLGGRLDATNIVSPVLSIIT
SISLDHTDILGDTLEAIAKEKAGIIKSHTPVIIGPQTPYLFIEKLAYQKNSFCHTIKNQFLLFDQENNAIAKKSLEVLSE
NWDIPQEAIFKGLEAKQPCRFEIINNSVILDVAHNPSGLTSLFAKTQTHFPNQPLRILFGLSKGKDLEGCLKILSRHGSA
FHLVEAPNGRGISFDLLKQGLMNTGIASTVIYSHINIQEGVKIAIKQAESSGQLLLICGSFFIMSQVRQALGMIEHLDSI
ELNEKSMNSY

Sequences:

>Translated_410_residues
MPYSELIQRLFALNLHGGIKLGLSNCEKLQTILKFPDHSFSSIHVAGTNGKGSVVAKIASALEYAGYRVGVYTSPHISSF
RERIRVNGIMISEADVEKILSQLFNLTAQYSIPATFFELTTFLALVYFSREKVDFAVLEAGLGGRLDATNIVSPVLSIIT
SISLDHTDILGDTLEAIAKEKAGIIKSHTPVIIGPQTPYLFIEKLAYQKNSFCHTIKNQFLLFDQENNAIAKKSLEVLSE
NWDIPQEAIFKGLEAKQPCRFEIINNSVILDVAHNPSGLTSLFAKTQTHFPNQPLRILFGLSKGKDLEGCLKILSRHGSA
FHLVEAPNGRGISFDLLKQGLMNTGIASTVIYSHINIQEGVKIAIKQAESSGQLLLICGSFFIMSQVRQALGMIEHLDSI
ELNEKSMNSY
>Mature_409_residues
PYSELIQRLFALNLHGGIKLGLSNCEKLQTILKFPDHSFSSIHVAGTNGKGSVVAKIASALEYAGYRVGVYTSPHISSFR
ERIRVNGIMISEADVEKILSQLFNLTAQYSIPATFFELTTFLALVYFSREKVDFAVLEAGLGGRLDATNIVSPVLSIITS
ISLDHTDILGDTLEAIAKEKAGIIKSHTPVIIGPQTPYLFIEKLAYQKNSFCHTIKNQFLLFDQENNAIAKKSLEVLSEN
WDIPQEAIFKGLEAKQPCRFEIINNSVILDVAHNPSGLTSLFAKTQTHFPNQPLRILFGLSKGKDLEGCLKILSRHGSAF
HLVEAPNGRGISFDLLKQGLMNTGIASTVIYSHINIQEGVKIAIKQAESSGQLLLICGSFFIMSQVRQALGMIEHLDSIE
LNEKSMNSY

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=175, Percent_Identity=39.4285714285714, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI66932984, Length=175, Percent_Identity=39.4285714285714, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI1788654, Length=404, Percent_Identity=30.9405940594059, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17553148, Length=159, Percent_Identity=38.9937106918239, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=159, Percent_Identity=38.9937106918239, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=159, Percent_Identity=38.9937106918239, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=420, Percent_Identity=27.1428571428571, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=172, Percent_Identity=39.5348837209302, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322718, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641571, Length=165, Percent_Identity=43.030303030303, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581568, Length=145, Percent_Identity=34.4827586206897, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45171; Mature: 45040

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYSELIQRLFALNLHGGIKLGLSNCEKLQTILKFPDHSFSSIHVAGTNGKGSVVAKIAS
CCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
ALEYAGYRVGVYTSPHISSFRERIRVNGIMISEADVEKILSQLFNLTAQYSIPATFFELT
HHHHCCEEEEEEECCHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TFLALVYFSREKVDFAVLEAGLGGRLDATNIVSPVLSIITSISLDHTDILGDTLEAIAKE
HHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KAGIIKSHTPVIIGPQTPYLFIEKLAYQKNSFCHTIKNQFLLFDQENNAIAKKSLEVLSE
HCCCEECCCCEEECCCCCHHHHHHHHHHCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
NWDIPQEAIFKGLEAKQPCRFEIINNSVILDVAHNPSGLTSLFAKTQTHFPNQPLRILFG
CCCCCHHHHHCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEE
LSKGKDLEGCLKILSRHGSAFHLVEAPNGRGISFDLLKQGLMNTGIASTVIYSHINIQEG
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHCC
VKIAIKQAESSGQLLLICGSFFIMSQVRQALGMIEHLDSIELNEKSMNSY
CEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
PYSELIQRLFALNLHGGIKLGLSNCEKLQTILKFPDHSFSSIHVAGTNGKGSVVAKIAS
CHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
ALEYAGYRVGVYTSPHISSFRERIRVNGIMISEADVEKILSQLFNLTAQYSIPATFFELT
HHHHCCEEEEEEECCHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TFLALVYFSREKVDFAVLEAGLGGRLDATNIVSPVLSIITSISLDHTDILGDTLEAIAKE
HHHHHHHHHCCCCCHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
KAGIIKSHTPVIIGPQTPYLFIEKLAYQKNSFCHTIKNQFLLFDQENNAIAKKSLEVLSE
HCCCEECCCCEEECCCCCHHHHHHHHHHCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
NWDIPQEAIFKGLEAKQPCRFEIINNSVILDVAHNPSGLTSLFAKTQTHFPNQPLRILFG
CCCCCHHHHHCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEE
LSKGKDLEGCLKILSRHGSAFHLVEAPNGRGISFDLLKQGLMNTGIASTVIYSHINIQEG
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHCC
VKIAIKQAESSGQLLLICGSFFIMSQVRQALGMIEHLDSIELNEKSMNSY
CEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]