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The map label for this gene is pcnB [H]

Identifier: 46446942

GI number: 46446942

Start: 1565019

End: 1566293

Strand: Direct

Name: pcnB [H]

Synonym: pc1308

Alternate gene names: 46446942

Gene position: 1565019-1566293 (Clockwise)

Preceding gene: 46446938

Following gene: 46446943

Centisome position: 64.82

GC content: 36.94

Gene sequence:

>1275_bases
GTGCAACCTAAGACCTATTTCGCTAAAGACCATGGAATTGATCCTGCTCTTATTGATCGCGATGCACTTTATGTTTTAGA
AAGGCTAAGACACGCTGGATTCACCGCTTATTTAGTCGGTGGAAGTGTGCGAGATCTTTTATTAAAACGAGTCCCTAAAG
ATTTTGATATTTCCACTTCTGCTCGACCCGAACAAATTAAAGCGATATTTCAAAGGCAGTGTATTTTAATTGGAAGACGA
TTTCGTTTAGCGCATATTCGTTTTGGTCATAAAATCATTGAAGTATCCACATTTCGCACCGGCGAGAACGATAGCGGACT
TATTCTTCATGATAATGAATGGGGCACCCCTGAAGAGGATGTTTTGCGCAGAGATTTCACTATTAATGGACTTTTTTACG
ATTCTTCCAACCATTCAATTATTGATTATGTCAATGGATGGGAAGACGTCCAAAAACATTTGTTGCGTACAATCGGTGAA
CCTGAAAAACGATTTAAGCAAGACCCAGTACGTTTGTTAAGACTTTTAAAATTTCATGCTCGTTATAACTTCAAAATAGA
AGAACAAACAGAGCAAGCCATTCATGTCTGCCGAATAGAAATCACAAAAAGTTCTCCTGCCAGAATTTTAGAAGAAATTC
TTCGTATGCTAGAATCAGGAGCTTCGGCTCCTTTTATTAAACTTTTGGCTGAATATGGAATCTTAAATATTTTGTTTCCT
AGTCTCACACAGCTTTTAAAAACAACTTACGGAAAAACAATTTTTCATTATTTAACTTGTGCGGATCAACTGTATCAACA
TAAAGGTAAAAATGTATTAGATCGAGCTATTTTAACAGCTTGCCTACTTTTTCCTGTTTTAGAACGTGAATTAGATAAAC
ACTATTTGAGTAAAAAACAAACTCCGCACATCGGAGAAATTACCCTTGTTGCTTCCTCTCTTGTTAAAGAGATTTTGATT
CAATCTTTTTCTCATTTTCCAAGGCGAATTACCTCAACGATGTTATCCATTTTAGTTGCGCAGTATCGTCTAACTCCCTT
GAGTGGAAAAAGGCATTATCGAGAAAAATTGTTTCGCCAAAAGGATTTTGAATTAGCATTAAAGTTTTTCAAGTTAAGGG
CTCTTGTTAATGAAAAATTAGTGGAGACATATTCTTCCATTCGCGATCAATATCGTCAATTCGTGCGACATGGAGAGAAA
CGTCATCACAATTATCATGGCCGGCCTGCTCATTCCCCTCATCAAGTTATATCTCATGACTGCGCAGCCACTTAA

Upstream 100 bases:

>100_bases
TTTGCAAAGACATTAGACAATTTATTTTTAAATTTCTTTGAAGGGGAATTCTTATCCAATAGATATTGAGAATGTTACAA
TTTTCAGGAGCTAATCTTTC

Downstream 100 bases:

>100_bases
TCAAGTTATCTATCCTCCTCAAGGAGGGCCTCCAATCTATTATCGTGGATCTGACTTATCAGAGGGAGCTAAACCAGCCG
TTGTTTATTTTGCCCTTTCT

Product: putative Poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MQPKTYFAKDHGIDPALIDRDALYVLERLRHAGFTAYLVGGSVRDLLLKRVPKDFDISTSARPEQIKAIFQRQCILIGRR
FRLAHIRFGHKIIEVSTFRTGENDSGLILHDNEWGTPEEDVLRRDFTINGLFYDSSNHSIIDYVNGWEDVQKHLLRTIGE
PEKRFKQDPVRLLRLLKFHARYNFKIEEQTEQAIHVCRIEITKSSPARILEEILRMLESGASAPFIKLLAEYGILNILFP
SLTQLLKTTYGKTIFHYLTCADQLYQHKGKNVLDRAILTACLLFPVLERELDKHYLSKKQTPHIGEITLVASSLVKEILI
QSFSHFPRRITSTMLSILVAQYRLTPLSGKRHYREKLFRQKDFELALKFFKLRALVNEKLVETYSSIRDQYRQFVRHGEK
RHHNYHGRPAHSPHQVISHDCAAT

Sequences:

>Translated_424_residues
MQPKTYFAKDHGIDPALIDRDALYVLERLRHAGFTAYLVGGSVRDLLLKRVPKDFDISTSARPEQIKAIFQRQCILIGRR
FRLAHIRFGHKIIEVSTFRTGENDSGLILHDNEWGTPEEDVLRRDFTINGLFYDSSNHSIIDYVNGWEDVQKHLLRTIGE
PEKRFKQDPVRLLRLLKFHARYNFKIEEQTEQAIHVCRIEITKSSPARILEEILRMLESGASAPFIKLLAEYGILNILFP
SLTQLLKTTYGKTIFHYLTCADQLYQHKGKNVLDRAILTACLLFPVLERELDKHYLSKKQTPHIGEITLVASSLVKEILI
QSFSHFPRRITSTMLSILVAQYRLTPLSGKRHYREKLFRQKDFELALKFFKLRALVNEKLVETYSSIRDQYRQFVRHGEK
RHHNYHGRPAHSPHQVISHDCAAT
>Mature_424_residues
MQPKTYFAKDHGIDPALIDRDALYVLERLRHAGFTAYLVGGSVRDLLLKRVPKDFDISTSARPEQIKAIFQRQCILIGRR
FRLAHIRFGHKIIEVSTFRTGENDSGLILHDNEWGTPEEDVLRRDFTINGLFYDSSNHSIIDYVNGWEDVQKHLLRTIGE
PEKRFKQDPVRLLRLLKFHARYNFKIEEQTEQAIHVCRIEITKSSPARILEEILRMLESGASAPFIKLLAEYGILNILFP
SLTQLLKTTYGKTIFHYLTCADQLYQHKGKNVLDRAILTACLLFPVLERELDKHYLSKKQTPHIGEITLVASSLVKEILI
QSFSHFPRRITSTMLSILVAQYRLTPLSGKRHYREKLFRQKDFELALKFFKLRALVNEKLVETYSSIRDQYRQFVRHGEK
RHHNYHGRPAHSPHQVISHDCAAT

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=190, Percent_Identity=30, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI87081691, Length=384, Percent_Identity=35.6770833333333, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=33.4841628959276, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=204, Percent_Identity=26.9607843137255, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=130, Percent_Identity=32.3076923076923, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281360112, Length=183, Percent_Identity=30.0546448087432, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21357337, Length=183, Percent_Identity=30.0546448087432, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 49394; Mature: 49394

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPKTYFAKDHGIDPALIDRDALYVLERLRHAGFTAYLVGGSVRDLLLKRVPKDFDISTS
CCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCC
ARPEQIKAIFQRQCILIGRRFRLAHIRFGHKIIEVSTFRTGENDSGLILHDNEWGTPEED
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEEEEEECCCCCCCEEEECCCCCCCHHH
VLRRDFTINGLFYDSSNHSIIDYVNGWEDVQKHLLRTIGEPEKRFKQDPVRLLRLLKFHA
HHHHCCEEEEEEEECCCCCEEHHHCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
RYNFKIEEQTEQAIHVCRIEITKSSPARILEEILRMLESGASAPFIKLLAEYGILNILFP
HCCCEEHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SLTQLLKTTYGKTIFHYLTCADQLYQHKGKNVLDRAILTACLLFPVLERELDKHYLSKKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPHIGEITLVASSLVKEILIQSFSHFPRRITSTMLSILVAQYRLTPLSGKRHYREKLFRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KDFELALKFFKLRALVNEKLVETYSSIRDQYRQFVRHGEKRHHNYHGRPAHSPHQVISHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CAAT
HCCC
>Mature Secondary Structure
MQPKTYFAKDHGIDPALIDRDALYVLERLRHAGFTAYLVGGSVRDLLLKRVPKDFDISTS
CCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCC
ARPEQIKAIFQRQCILIGRRFRLAHIRFGHKIIEVSTFRTGENDSGLILHDNEWGTPEED
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEEEEEECCCCCCCEEEECCCCCCCHHH
VLRRDFTINGLFYDSSNHSIIDYVNGWEDVQKHLLRTIGEPEKRFKQDPVRLLRLLKFHA
HHHHCCEEEEEEEECCCCCEEHHHCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHH
RYNFKIEEQTEQAIHVCRIEITKSSPARILEEILRMLESGASAPFIKLLAEYGILNILFP
HCCCEEHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SLTQLLKTTYGKTIFHYLTCADQLYQHKGKNVLDRAILTACLLFPVLERELDKHYLSKKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPHIGEITLVASSLVKEILIQSFSHFPRRITSTMLSILVAQYRLTPLSGKRHYREKLFRQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KDFELALKFFKLRALVNEKLVETYSSIRDQYRQFVRHGEKRHHNYHGRPAHSPHQVISHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
CAAT
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]