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The map label for this gene is lysS [H]

Identifier: 46446862

GI number: 46446862

Start: 1462049

End: 1463638

Strand: Direct

Name: lysS [H]

Synonym: pc1228

Alternate gene names: 46446862

Gene position: 1462049-1463638 (Clockwise)

Preceding gene: 46446861

Following gene: 46446863

Centisome position: 60.55

GC content: 37.42

Gene sequence:

>1590_bases
ATGAGTTTTATGACAAAAAAACCTGATTATCACCAACATGAAGAATACCAAAATCGCTTACGTAAACTTGTTGAAATACG
TCAATGCAAGATAGATCCTTATCCCGCTAAATATACACCAACTCATACAGCAGAATCTCTTCATCAAGAATACGATACAA
AACAAATTGGACATAGTGAAGATGCAGCTTCAGGATCCACACCTTTTGTTTGTTTGGCTGGCCGTCTTGTCCTTTTTCGT
TCCATGGGAAAAAATGCCTTTGGTCATCTACAAGATGAAACAGGACGCATACAAATCATGTTTAATCGGGACCAAACGCT
AGTCACGAACTTTGAACCTAGTTCTGAAGAACCCGAATTATCTCCATATAAATTTATCGAAAAAAAAATCGATCTCGGAG
ATATCATTGGAGTTGAAGGCTACTTATTCCATACTCAAAAAGGAGAACTAACTGTTTTTGTCAAACAGCTGACTCTTCTT
TGTAAAACTCTACTTCCGCTTGCCGATAAACATGGTGGACTGGCTGATAAAGAATTGCGTTATCGTAAACGTTGGTTAGA
TTTGATTTCCCATTTAGATGTAGCTAAAGTTTTTCGTATCCGTAGTCATATCTTGAAAGAAATCCGATCTTACTTTGACC
AGTATCAATTCCTAGAAGTAGAAACACCAATCCTACAAAGCATTTATGGAGGAGCAGAAGCCCGCCCCTTTATCACAAAA
TTAAATGCGCTGGATCAAGAAATGTTTTTACGCATTTCTTTAGAAATTCCTCTTAAAAAGTTAATTGTAGGAGGGATGCA
TAAAGTTTATGAAATGGCACGTGTATTTCGTAATGAAGGCATTGATCGCAATCATAATCCTGAATTTACCTTGTTAGAAG
CTTATGCTTCCTATTGGGATTATTTCGATATGATGAACTTTGTGGAAAATCTTTTCGAAAAGCTTGCTCTAGACCTCTAT
GGAACCACGAAAGTTCCTTATACAGCACATGAAGGAGCTGAAACAATTTATATCGATATGAAAGCTCCTTGGATTCGATT
GACTATGAAAGAAAGCATTAAAAAATATGCTAATCTCGATCCTAATGTTTTATCTGATGAAGAGATGCGACATTTATTGC
TCGAGAGTGCACATTGTGATCCTAAAAAAATTAAAGGATTGAGTCGCGGACTTCTGATTGCGGCTTTATTTGAGGTTTTT
GTAGAACCTCATTTAATTCAACCCCACCACATTTACGACCATCCCATTGAGACAACGCCTCTTTGTAAACCACATCGTGA
TCTTAAATACCGCGAAGAAGGACTTGTTGAACGTTTTGAAAGTTTTATTTTAACACAAGAAGTGTGCAATTCTTACAGCG
AATTGAACGATCCTGAAATTCAACGTCATTTGCTTGAGCTACAGTCAGATCGCCGTGAAGCTGGTGATGAAGAAGCTAGT
CCCTTGGATGAAGAATTTATTGAAGCGATTTGCCAAGGTATGCCTCCCACAGGGGGAATTGGAATTGGAATTGACCGACT
GGTAATGATATTAACTAATTCTCATTCGATTCGGGATGTCCTTTATTTTCCTTGGATGAAAACAATTTAA

Upstream 100 bases:

>100_bases
AGCTTATGCACGTCTTGAATACAGCCGCAGTGACGAGCAAAAACCCATTCAAGTTCGTATTCCCAATTTGGATTTAGAAA
ATACCCCTTTAAAGAAATAG

Downstream 100 bases:

>100_bases
AACACATCGCTATAACGGGCTAATCTGAAAGGAGAATTTTATGAATTTTTGGGATTCCCACGGTATTTTCTTTTTACTCT
TTATCACGCTATTTCCTAGG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSFMTKKPDYHQHEEYQNRLRKLVEIRQCKIDPYPAKYTPTHTAESLHQEYDTKQIGHSEDAASGSTPFVCLAGRLVLFR
SMGKNAFGHLQDETGRIQIMFNRDQTLVTNFEPSSEEPELSPYKFIEKKIDLGDIIGVEGYLFHTQKGELTVFVKQLTLL
CKTLLPLADKHGGLADKELRYRKRWLDLISHLDVAKVFRIRSHILKEIRSYFDQYQFLEVETPILQSIYGGAEARPFITK
LNALDQEMFLRISLEIPLKKLIVGGMHKVYEMARVFRNEGIDRNHNPEFTLLEAYASYWDYFDMMNFVENLFEKLALDLY
GTTKVPYTAHEGAETIYIDMKAPWIRLTMKESIKKYANLDPNVLSDEEMRHLLLESAHCDPKKIKGLSRGLLIAALFEVF
VEPHLIQPHHIYDHPIETTPLCKPHRDLKYREEGLVERFESFILTQEVCNSYSELNDPEIQRHLLELQSDRREAGDEEAS
PLDEEFIEAICQGMPPTGGIGIGIDRLVMILTNSHSIRDVLYFPWMKTI

Sequences:

>Translated_529_residues
MSFMTKKPDYHQHEEYQNRLRKLVEIRQCKIDPYPAKYTPTHTAESLHQEYDTKQIGHSEDAASGSTPFVCLAGRLVLFR
SMGKNAFGHLQDETGRIQIMFNRDQTLVTNFEPSSEEPELSPYKFIEKKIDLGDIIGVEGYLFHTQKGELTVFVKQLTLL
CKTLLPLADKHGGLADKELRYRKRWLDLISHLDVAKVFRIRSHILKEIRSYFDQYQFLEVETPILQSIYGGAEARPFITK
LNALDQEMFLRISLEIPLKKLIVGGMHKVYEMARVFRNEGIDRNHNPEFTLLEAYASYWDYFDMMNFVENLFEKLALDLY
GTTKVPYTAHEGAETIYIDMKAPWIRLTMKESIKKYANLDPNVLSDEEMRHLLLESAHCDPKKIKGLSRGLLIAALFEVF
VEPHLIQPHHIYDHPIETTPLCKPHRDLKYREEGLVERFESFILTQEVCNSYSELNDPEIQRHLLELQSDRREAGDEEAS
PLDEEFIEAICQGMPPTGGIGIGIDRLVMILTNSHSIRDVLYFPWMKTI
>Mature_528_residues
SFMTKKPDYHQHEEYQNRLRKLVEIRQCKIDPYPAKYTPTHTAESLHQEYDTKQIGHSEDAASGSTPFVCLAGRLVLFRS
MGKNAFGHLQDETGRIQIMFNRDQTLVTNFEPSSEEPELSPYKFIEKKIDLGDIIGVEGYLFHTQKGELTVFVKQLTLLC
KTLLPLADKHGGLADKELRYRKRWLDLISHLDVAKVFRIRSHILKEIRSYFDQYQFLEVETPILQSIYGGAEARPFITKL
NALDQEMFLRISLEIPLKKLIVGGMHKVYEMARVFRNEGIDRNHNPEFTLLEAYASYWDYFDMMNFVENLFEKLALDLYG
TTKVPYTAHEGAETIYIDMKAPWIRLTMKESIKKYANLDPNVLSDEEMRHLLLESAHCDPKKIKGLSRGLLIAALFEVFV
EPHLIQPHHIYDHPIETTPLCKPHRDLKYREEGLVERFESFILTQEVCNSYSELNDPEIQRHLLELQSDRREAGDEEASP
LDEEFIEAICQGMPPTGGIGIGIDRLVMILTNSHSIRDVLYFPWMKTI

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=523, Percent_Identity=39.5793499043977, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI194272210, Length=523, Percent_Identity=39.5793499043977, Blast_Score=332, Evalue=7e-91,
Organism=Homo sapiens, GI40789249, Length=316, Percent_Identity=25.3164556962025, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790571, Length=516, Percent_Identity=43.6046511627907, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1789256, Length=518, Percent_Identity=42.8571428571429, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=28.3132530120482, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788173, Length=260, Percent_Identity=24.2307692307692, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=537, Percent_Identity=37.9888268156425, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17535927, Length=537, Percent_Identity=37.9888268156425, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=521, Percent_Identity=38.3877159309021, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6320242, Length=532, Percent_Identity=37.9699248120301, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324256, Length=545, Percent_Identity=30.6422018348624, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24640849, Length=510, Percent_Identity=37.2549019607843, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=37.2549019607843, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24584738, Length=253, Percent_Identity=25.6916996047431, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 61403; Mature: 61272

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFMTKKPDYHQHEEYQNRLRKLVEIRQCKIDPYPAKYTPTHTAESLHQEYDTKQIGHSE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
DAASGSTPFVCLAGRLVLFRSMGKNAFGHLQDETGRIQIMFNRDQTLVTNFEPSSEEPEL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCC
SPYKFIEKKIDLGDIIGVEGYLFHTQKGELTVFVKQLTLLCKTLLPLADKHGGLADKELR
CHHHHHHHHCCCHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YRKRWLDLISHLDVAKVFRIRSHILKEIRSYFDQYQFLEVETPILQSIYGGAEARPFITK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCCHHHHH
LNALDQEMFLRISLEIPLKKLIVGGMHKVYEMARVFRNEGIDRNHNPEFTLLEAYASYWD
HHHHCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
YFDMMNFVENLFEKLALDLYGTTKVPYTAHEGAETIYIDMKAPWIRLTMKESIKKYANLD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCEEEEHHHHHHHHHHCCC
PNVLSDEEMRHLLLESAHCDPKKIKGLSRGLLIAALFEVFVEPHLIQPHHIYDHPIETTP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LCKPHRDLKYREEGLVERFESFILTQEVCNSYSELNDPEIQRHLLELQSDRREAGDEEAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
PLDEEFIEAICQGMPPTGGIGIGIDRLVMILTNSHSIRDVLYFPWMKTI
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCHHHCC
>Mature Secondary Structure 
SFMTKKPDYHQHEEYQNRLRKLVEIRQCKIDPYPAKYTPTHTAESLHQEYDTKQIGHSE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
DAASGSTPFVCLAGRLVLFRSMGKNAFGHLQDETGRIQIMFNRDQTLVTNFEPSSEEPEL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCC
SPYKFIEKKIDLGDIIGVEGYLFHTQKGELTVFVKQLTLLCKTLLPLADKHGGLADKELR
CHHHHHHHHCCCHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YRKRWLDLISHLDVAKVFRIRSHILKEIRSYFDQYQFLEVETPILQSIYGGAEARPFITK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCCHHHHH
LNALDQEMFLRISLEIPLKKLIVGGMHKVYEMARVFRNEGIDRNHNPEFTLLEAYASYWD
HHHHCHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
YFDMMNFVENLFEKLALDLYGTTKVPYTAHEGAETIYIDMKAPWIRLTMKESIKKYANLD
HHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEEECCCCEEEEHHHHHHHHHHCCC
PNVLSDEEMRHLLLESAHCDPKKIKGLSRGLLIAALFEVFVEPHLIQPHHIYDHPIETTP
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
LCKPHRDLKYREEGLVERFESFILTQEVCNSYSELNDPEIQRHLLELQSDRREAGDEEAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
PLDEEFIEAICQGMPPTGGIGIGIDRLVMILTNSHSIRDVLYFPWMKTI
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA