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The map label for this gene is pfkA [H]

Identifier: 46446514

GI number: 46446514

Start: 1062582

End: 1064243

Strand: Direct

Name: pfkA [H]

Synonym: pc0880

Alternate gene names: 46446514

Gene position: 1062582-1064243 (Clockwise)

Preceding gene: 46446513

Following gene: 46446515

Centisome position: 44.01

GC content: 36.58

Gene sequence:

>1662_bases
ATGATCATGAGCATTGAGGTAGCAATGATTTCTTTTAGCCCGTTGCAAATTCACCGTTTAAAGTATCAACCTCAGCTTCC
CCTCCTTTTGCAAGATTTAAGTGTAATTGCAGCAAATTTTGAAAAATCAATTTGTCTCTCTGATTCTGCCTTGCAAGAGC
AGTTTCCACAAACATTTCAGCAACCTATTATTACTTTTCGCCGCGGCAACAAAAAAGAAGCCACACCTTTAAAAGTAGGT
GTCGTGTTATCTGGAGGTCAGGCTCCAGGAGGACATAATGTGATTGCCGGTTTATTTGATGCCTTAAAGCAAATCCATCC
AGAAAGCATATTACTGGGTTTCCTTGACGGTCCTTTAGGAATATTGAAGAACACCTCTATCGAATTAACAAAAATGGTGA
TCGATTCCTATCGCAATCAAGGAGGATTCGATATGATCGGATCTGGGAGAACAAAAATCGAAACTCCCGCTCAATTAGAA
GCAGCCATGTTAACTGTTCAAACTTTAGCGCTGGATGGATTAGTCATTATTGGAGGAGACGATTCTAACACCAATGCAGC
TATTTTAGCAGAATATTTTAAACAAGTTGGCGTAAAAACTTCTGTTATTGGAGTTCCAAAAACAATTGACGGGGACCTTA
AAAATGAATTTATAGACATTTCATTTGGATTTGATACTGCTAGTAAAATATATGCGGAATGTATTGGAAATATTTTGAAA
GATGCTTTATCAGCAAAAAAATATTATCATTTTATTAAATTGATGGGCCGCTCTGCTTCTCATCTTGCTCTCGAGTGCGC
TTTATTGACACACCCTAATTTAGTTTTTATTAGTGAAGAAGTTGCAGAAAAGAAACAAACTCTTCAAGAAATTGTTGGGC
AAATCACTACCATGATTTGTAAAAGAGCTGAAAAAAATCTTCACTACGGAGTGATTTTAATACCGGAAGGTTTAATAGAA
TCTATCTCTGAAATTGGAGCGTTGATTCAGAAGCTCAATGAATTACTTTTTTCTCACTCAGAAAAAATAGAAGGATTATT
AATAACGGAAGAAAAAGATAATTATATTATTTCTCAGCTTACTTCAAACGAAGCAAAAACATTTTTAGCCCTCCCAAAAG
AGGTACGCGATCAACTTTTGTTAGAAAGAGACTCACATGGAAATGTACAGGTATCGAAAATAGAAACGGAACGTTTATTA
ATAAAGTTAGTAGAAAAAGAGCTAAAACGACTTAAGCAAGAAGGCATTTATAAAGGAACATTTAGCCCTCAACCTCAATT
TTGTGGTTATGAAGGACGGTCTGGATTACCAAGCAACTTTGATTGTCAATATTGCTATGCTCTCGGATTTGTAGCTACTT
TGTTGGTTCAATCCGGTGTTACGGGTTATATGAGTTGTCTAAAAAAATTATCAGAGCCTATTGAGAGATGGAGCCCTATG
GGAGTGCCAATTTATCGAATGCTTCAGATAGAAAAAAGACAAGGTAAACAAAAATCTGTGATTCGAAAATTTCTTGTCGA
TTTGCAAGGACCGTTTTTTAAACAGTATGCCTCAGTAAGAGACCAATGGCAAATTACTGATGACTATCGTTCGCCCGGCC
CCATTCAGTTTGAAGGACCTAAAAATCTGACTGAGGTGAAGCCTCTTTCACTTTTGCTTTAA

Upstream 100 bases:

>100_bases
TATCTTGCTCCCGCAGTCTGATCATGATTTTTCGGATAAAAGTGAGCGAGAAAAGGCACTTCAGGAAACCTGTACTTGGT
TTAAAGAAACTTTGTAAAAT

Downstream 100 bases:

>100_bases
AAAAGTTTGGCAAAGAATTTTTAGAGCGCTATATAGAAATTAAATAAGTTTTGAAAGAAGTTTAACAAATTTGAACGGCA
TCTTTAGTTTTTTTGAGGCA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MIMSIEVAMISFSPLQIHRLKYQPQLPLLLQDLSVIAANFEKSICLSDSALQEQFPQTFQQPIITFRRGNKKEATPLKVG
VVLSGGQAPGGHNVIAGLFDALKQIHPESILLGFLDGPLGILKNTSIELTKMVIDSYRNQGGFDMIGSGRTKIETPAQLE
AAMLTVQTLALDGLVIIGGDDSNTNAAILAEYFKQVGVKTSVIGVPKTIDGDLKNEFIDISFGFDTASKIYAECIGNILK
DALSAKKYYHFIKLMGRSASHLALECALLTHPNLVFISEEVAEKKQTLQEIVGQITTMICKRAEKNLHYGVILIPEGLIE
SISEIGALIQKLNELLFSHSEKIEGLLITEEKDNYIISQLTSNEAKTFLALPKEVRDQLLLERDSHGNVQVSKIETERLL
IKLVEKELKRLKQEGIYKGTFSPQPQFCGYEGRSGLPSNFDCQYCYALGFVATLLVQSGVTGYMSCLKKLSEPIERWSPM
GVPIYRMLQIEKRQGKQKSVIRKFLVDLQGPFFKQYASVRDQWQITDDYRSPGPIQFEGPKNLTEVKPLSLLL

Sequences:

>Translated_553_residues
MIMSIEVAMISFSPLQIHRLKYQPQLPLLLQDLSVIAANFEKSICLSDSALQEQFPQTFQQPIITFRRGNKKEATPLKVG
VVLSGGQAPGGHNVIAGLFDALKQIHPESILLGFLDGPLGILKNTSIELTKMVIDSYRNQGGFDMIGSGRTKIETPAQLE
AAMLTVQTLALDGLVIIGGDDSNTNAAILAEYFKQVGVKTSVIGVPKTIDGDLKNEFIDISFGFDTASKIYAECIGNILK
DALSAKKYYHFIKLMGRSASHLALECALLTHPNLVFISEEVAEKKQTLQEIVGQITTMICKRAEKNLHYGVILIPEGLIE
SISEIGALIQKLNELLFSHSEKIEGLLITEEKDNYIISQLTSNEAKTFLALPKEVRDQLLLERDSHGNVQVSKIETERLL
IKLVEKELKRLKQEGIYKGTFSPQPQFCGYEGRSGLPSNFDCQYCYALGFVATLLVQSGVTGYMSCLKKLSEPIERWSPM
GVPIYRMLQIEKRQGKQKSVIRKFLVDLQGPFFKQYASVRDQWQITDDYRSPGPIQFEGPKNLTEVKPLSLLL
>Mature_553_residues
MIMSIEVAMISFSPLQIHRLKYQPQLPLLLQDLSVIAANFEKSICLSDSALQEQFPQTFQQPIITFRRGNKKEATPLKVG
VVLSGGQAPGGHNVIAGLFDALKQIHPESILLGFLDGPLGILKNTSIELTKMVIDSYRNQGGFDMIGSGRTKIETPAQLE
AAMLTVQTLALDGLVIIGGDDSNTNAAILAEYFKQVGVKTSVIGVPKTIDGDLKNEFIDISFGFDTASKIYAECIGNILK
DALSAKKYYHFIKLMGRSASHLALECALLTHPNLVFISEEVAEKKQTLQEIVGQITTMICKRAEKNLHYGVILIPEGLIE
SISEIGALIQKLNELLFSHSEKIEGLLITEEKDNYIISQLTSNEAKTFLALPKEVRDQLLLERDSHGNVQVSKIETERLL
IKLVEKELKRLKQEGIYKGTFSPQPQFCGYEGRSGLPSNFDCQYCYALGFVATLLVQSGVTGYMSCLKKLSEPIERWSPM
GVPIYRMLQIEKRQGKQKSVIRKFLVDLQGPFFKQYASVRDQWQITDDYRSPGPIQFEGPKNLTEVKPLSLLL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=256, Percent_Identity=28.125, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 61545; Mature: 61545

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMSIEVAMISFSPLQIHRLKYQPQLPLLLQDLSVIAANFEKSICLSDSALQEQFPQTFQ
CEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHH
QPIITFRRGNKKEATPLKVGVVLSGGQAPGGHNVIAGLFDALKQIHPESILLGFLDGPLG
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHH
ILKNTSIELTKMVIDSYRNQGGFDMIGSGRTKIETPAQLEAAMLTVQTLALDGLVIIGGD
HHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECC
DSNTNAAILAEYFKQVGVKTSVIGVPKTIDGDLKNEFIDISFGFDTASKIYAECIGNILK
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DALSAKKYYHFIKLMGRSASHLALECALLTHPNLVFISEEVAEKKQTLQEIVGQITTMIC
HHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
KRAEKNLHYGVILIPEGLIESISEIGALIQKLNELLFSHSEKIEGLLITEEKDNYIISQL
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEHH
TSNEAKTFLALPKEVRDQLLLERDSHGNVQVSKIETERLLIKLVEKELKRLKQEGIYKGT
HCCCCHHHHHCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCEECC
FSPQPQFCGYEGRSGLPSNFDCQYCYALGFVATLLVQSGVTGYMSCLKKLSEPIERWSPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
GVPIYRMLQIEKRQGKQKSVIRKFLVDLQGPFFKQYASVRDQWQITDDYRSPGPIQFEGP
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCCCCCCCEEECCC
KNLTEVKPLSLLL
CCCCCCCCHHHCC
>Mature Secondary Structure
MIMSIEVAMISFSPLQIHRLKYQPQLPLLLQDLSVIAANFEKSICLSDSALQEQFPQTFQ
CEEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHH
QPIITFRRGNKKEATPLKVGVVLSGGQAPGGHNVIAGLFDALKQIHPESILLGFLDGPLG
HHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHH
ILKNTSIELTKMVIDSYRNQGGFDMIGSGRTKIETPAQLEAAMLTVQTLALDGLVIIGGD
HHCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECC
DSNTNAAILAEYFKQVGVKTSVIGVPKTIDGDLKNEFIDISFGFDTASKIYAECIGNILK
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DALSAKKYYHFIKLMGRSASHLALECALLTHPNLVFISEEVAEKKQTLQEIVGQITTMIC
HHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
KRAEKNLHYGVILIPEGLIESISEIGALIQKLNELLFSHSEKIEGLLITEEKDNYIISQL
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEHH
TSNEAKTFLALPKEVRDQLLLERDSHGNVQVSKIETERLLIKLVEKELKRLKQEGIYKGT
HCCCCHHHHHCCHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCEECC
FSPQPQFCGYEGRSGLPSNFDCQYCYALGFVATLLVQSGVTGYMSCLKKLSEPIERWSPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
GVPIYRMLQIEKRQGKQKSVIRKFLVDLQGPFFKQYASVRDQWQITDDYRSPGPIQFEGP
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEECCCCCCCCCEEECCC
KNLTEVKPLSLLL
CCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA