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The map label for this gene is braB [H]

Identifier: 46446401

GI number: 46446401

Start: 948889

End: 950070

Strand: Reverse

Name: braB [H]

Synonym: pc0767

Alternate gene names: 46446401

Gene position: 950070-948889 (Counterclockwise)

Preceding gene: 46446409

Following gene: 46446398

Centisome position: 39.35

GC content: 33.76

Gene sequence:

>1182_bases
ATGAAAAAGCCTTCACCAAGGTCAAATACTTTAGCGACTGGCCTTGCTATGTTCTCAATGTTTTTTGGAGCAGGAAATGT
TGTATTCCCGCTCGCATTAGGGCAACATGCAAAAGATCAAAATATATATGCTATTATTGGACTTCTTATTACAGCTGTCG
GAGTTCCTTTTCTCGGTCTGATTGCAATGACTCTTTTTGACGGAAATTACCGAGCTTTTTTTAATCGTTTAGGCAAGATT
CCTGGTTTTTTAATAGCTCTTACTATCATGGGATTAATTGGCCCATTTGGAGCTTTACCACGATGTATCGCTCTTTCCTA
TTCTACGATTAGTCTTGCTATTCCTCATTTAGAACTGATTCCTTTTAGTATTATTTCTTGTATTTTAATTTATTTATTCA
CTTTTCGCAAAAATAACATTATCGATGTATTAGGGTATGTCTTAACTCCCTTTCTCTTATTTTCTTTGCTTATTATTATT
TTAAAGGGTTTTTGGGAAGCAAATGGTTTGCCTGCTTCAATTCATCAACCTTATGAAACATTTTTAGTAGGACTACAAGA
AGGCTACCAAACAATGGATCTTTTAGGAGCATTCTTTTTTTGTTCAGTCGTACTAGCTTGCTTGAAAAAAGGGGTTGAAC
CACAAACGCATGAACATGTTAATTATAAAAAAATGATAATATTAACTCTAAAAGCTAGCTGTATTGGGGCATTTTTGCTA
GGTTTGGTTTACTTAGGATTTAGCTATGTTGCTGCCTATAACAGCGAAAATCTCGCTGAAGTTTCTAAAGAACAAGTTTT
AGCAACTTTATCTCACCAAATTTTAGGTCCGAATGCAGGAATTGTCGCTTCGATTGCCGTCGCATTAGCCTGTCTAACTA
CAGCCATTGCACTTGCAGTTGTTTTTGCAGAATTTTTACATTTTGATGTTTCTAAAGGAAAGATTTCTTATCATAACTCT
CTCATTCTCACTCTAGTTGTTTCTTTCATTTTTTCAACATTGAATTTTACAGGAATTATTGCTTTTTTAGCTCCTATTTT
AGAAGTTTGTTATCCTGCGTTAATTGTTTTATGTATCGTCAATATCGGTTATAAACTTGTTCACTTTAAACCAGTTAAAA
TACCTGTATTTAGTATATTTGCTTTATCGCTTTTATACCATGCCTCAAGTTACTTAAAGTAA

Upstream 100 bases:

>100_bases
TCCATTTTAAATTAATTCATTATGATTCGTTACTATTATTAAACTATTTTGATTTTTTATCGATAAACTCATCTTACAGA
GTGAAAATTAAAGGAAATAT

Downstream 100 bases:

>100_bases
TATTTATCGCTTAAATCAGAGTTGTTTTCGCAGCTCTGATTTAAATTCATTATCTTCAAATAAAATGCGCGATATTCTAA
ATTTCACTTTATATTCATGC

Product: putative branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKKPSPRSNTLATGLAMFSMFFGAGNVVFPLALGQHAKDQNIYAIIGLLITAVGVPFLGLIAMTLFDGNYRAFFNRLGKI
PGFLIALTIMGLIGPFGALPRCIALSYSTISLAIPHLELIPFSIISCILIYLFTFRKNNIIDVLGYVLTPFLLFSLLIII
LKGFWEANGLPASIHQPYETFLVGLQEGYQTMDLLGAFFFCSVVLACLKKGVEPQTHEHVNYKKMIILTLKASCIGAFLL
GLVYLGFSYVAAYNSENLAEVSKEQVLATLSHQILGPNAGIVASIAVALACLTTAIALAVVFAEFLHFDVSKGKISYHNS
LILTLVVSFIFSTLNFTGIIAFLAPILEVCYPALIVLCIVNIGYKLVHFKPVKIPVFSIFALSLLYHASSYLK

Sequences:

>Translated_393_residues
MKKPSPRSNTLATGLAMFSMFFGAGNVVFPLALGQHAKDQNIYAIIGLLITAVGVPFLGLIAMTLFDGNYRAFFNRLGKI
PGFLIALTIMGLIGPFGALPRCIALSYSTISLAIPHLELIPFSIISCILIYLFTFRKNNIIDVLGYVLTPFLLFSLLIII
LKGFWEANGLPASIHQPYETFLVGLQEGYQTMDLLGAFFFCSVVLACLKKGVEPQTHEHVNYKKMIILTLKASCIGAFLL
GLVYLGFSYVAAYNSENLAEVSKEQVLATLSHQILGPNAGIVASIAVALACLTTAIALAVVFAEFLHFDVSKGKISYHNS
LILTLVVSFIFSTLNFTGIIAFLAPILEVCYPALIVLCIVNIGYKLVHFKPVKIPVFSIFALSLLYHASSYLK
>Mature_393_residues
MKKPSPRSNTLATGLAMFSMFFGAGNVVFPLALGQHAKDQNIYAIIGLLITAVGVPFLGLIAMTLFDGNYRAFFNRLGKI
PGFLIALTIMGLIGPFGALPRCIALSYSTISLAIPHLELIPFSIISCILIYLFTFRKNNIIDVLGYVLTPFLLFSLLIII
LKGFWEANGLPASIHQPYETFLVGLQEGYQTMDLLGAFFFCSVVLACLKKGVEPQTHEHVNYKKMIILTLKASCIGAFLL
GLVYLGFSYVAAYNSENLAEVSKEQVLATLSHQILGPNAGIVASIAVALACLTTAIALAVVFAEFLHFDVSKGKISYHNS
LILTLVVSFIFSTLNFTGIIAFLAPILEVCYPALIVLCIVNIGYKLVHFKPVKIPVFSIFALSLLYHASSYLK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=373, Percent_Identity=27.0777479892761, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 42838; Mature: 42838

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPSPRSNTLATGLAMFSMFFGAGNVVFPLALGQHAKDQNIYAIIGLLITAVGVPFLGL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IAMTLFDGNYRAFFNRLGKIPGFLIALTIMGLIGPFGALPRCIALSYSTISLAIPHLELI
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
PFSIISCILIYLFTFRKNNIIDVLGYVLTPFLLFSLLIIILKGFWEANGLPASIHQPYET
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHH
FLVGLQEGYQTMDLLGAFFFCSVVLACLKKGVEPQTHEHVNYKKMIILTLKASCIGAFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEHHHHHHHHHHH
GLVYLGFSYVAAYNSENLAEVSKEQVLATLSHQILGPNAGIVASIAVALACLTTAIALAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VFAEFLHFDVSKGKISYHNSLILTLVVSFIFSTLNFTGIIAFLAPILEVCYPALIVLCIV
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIGYKLVHFKPVKIPVFSIFALSLLYHASSYLK
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKPSPRSNTLATGLAMFSMFFGAGNVVFPLALGQHAKDQNIYAIIGLLITAVGVPFLGL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IAMTLFDGNYRAFFNRLGKIPGFLIALTIMGLIGPFGALPRCIALSYSTISLAIPHLELI
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
PFSIISCILIYLFTFRKNNIIDVLGYVLTPFLLFSLLIIILKGFWEANGLPASIHQPYET
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHH
FLVGLQEGYQTMDLLGAFFFCSVVLACLKKGVEPQTHEHVNYKKMIILTLKASCIGAFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEHHHHHHHHHHH
GLVYLGFSYVAAYNSENLAEVSKEQVLATLSHQILGPNAGIVASIAVALACLTTAIALAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VFAEFLHFDVSKGKISYHNSLILTLVVSFIFSTLNFTGIIAFLAPILEVCYPALIVLCIV
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIGYKLVHFKPVKIPVFSIFALSLLYHASSYLK
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]