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The map label for this gene is truB

Identifier: 46446393

GI number: 46446393

Start: 936827

End: 937615

Strand: Reverse

Name: truB

Synonym: pc0759

Alternate gene names: 46446393

Gene position: 937615-936827 (Counterclockwise)

Preceding gene: 46446394

Following gene: 46446392

Centisome position: 38.83

GC content: 38.78

Gene sequence:

>789_bases
TTGATAAACACATGCGCATTGAAGAATTACTTGGAAAAATCACAAAAGAAAGAGAGTCCCGCCAAGGAGACAATAGTGAC
CAGCTTGAACAGGAGCCTTAGCACTCCTCCTTCTCATTTAGAAGGAATTCTACTTATCAATAAACCTAAGGGTAAAACAT
CCTTTAGCCTTGTTAGAGATCTTCGCAAAAGACTTGGAGTAAAAAAAATTGGGCATGCCGGCACTTTAGACCCTTTTGCA
ACAGGCGTCATGGTGATGTTAGTCGGTCGTAATTACACGAGACTTTCCGATCAATTTCTTTTGAGTGATAAAGAATACAT
TGCTGAAGCTTATTTAGGAGTTGTTACAGATTCTTACGATTGTGAAGGCCAGGTATTATCTCAATCAAATATTATTCCGA
CTCTAGAACAAATCAAAGAAGCCTTTTCTTTGTTTCAAGGAAAAATAGAACAAGTCCCGCCTATGTTTTCTGCCAAAAAG
CAACAAGGGAAAAAGTTGTATGAATTAGCAAGACAGGGAATAGTGGTAGAGAGACAGCCCGTAAAAATTTCTATTCATAC
GGAGTTACTTTCGTACAACTATCCCTATTTAAATTTTCGCATCGAATGTAGCAAAGGAACCTATATACGTAGCATCGCGT
ATGACTTGGGTACAAAACTCGGTTGTGGAGCCCATTTATCCAATTTAACCCGCACGCGAAGCGGAGCTTTTTGTTTAGAA
AATTGCCTTAATGGGGCAGAGATTCATACCGTCACCAATTTAGAACAAAATTTGATTCGTAACGACTAA

Upstream 100 bases:

>100_bases
GAAGCTTTAAATTCTGCTGCTGGATTTATTGCAGTTAATGCTTCACAAAAAGTTGTTATGCGCTATTTTCCAGAGCTTAA
TTTTAAATTAGATGATAGCG

Downstream 100 bases:

>100_bases
AAATAATTCCTCAATGAAATTGTATAGTAAACTTGAAGAAGTTTTCGATAAAAAAATCCCTCAAATTCTCACGATAGGGA
ATTTTGATGGGGTTCACTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MINTCALKNYLEKSQKKESPAKETIVTSLNRSLSTPPSHLEGILLINKPKGKTSFSLVRDLRKRLGVKKIGHAGTLDPFA
TGVMVMLVGRNYTRLSDQFLLSDKEYIAEAYLGVVTDSYDCEGQVLSQSNIIPTLEQIKEAFSLFQGKIEQVPPMFSAKK
QQGKKLYELARQGIVVERQPVKISIHTELLSYNYPYLNFRIECSKGTYIRSIAYDLGTKLGCGAHLSNLTRTRSGAFCLE
NCLNGAEIHTVTNLEQNLIRND

Sequences:

>Translated_262_residues
MINTCALKNYLEKSQKKESPAKETIVTSLNRSLSTPPSHLEGILLINKPKGKTSFSLVRDLRKRLGVKKIGHAGTLDPFA
TGVMVMLVGRNYTRLSDQFLLSDKEYIAEAYLGVVTDSYDCEGQVLSQSNIIPTLEQIKEAFSLFQGKIEQVPPMFSAKK
QQGKKLYELARQGIVVERQPVKISIHTELLSYNYPYLNFRIECSKGTYIRSIAYDLGTKLGCGAHLSNLTRTRSGAFCLE
NCLNGAEIHTVTNLEQNLIRND
>Mature_262_residues
MINTCALKNYLEKSQKKESPAKETIVTSLNRSLSTPPSHLEGILLINKPKGKTSFSLVRDLRKRLGVKKIGHAGTLDPFA
TGVMVMLVGRNYTRLSDQFLLSDKEYIAEAYLGVVTDSYDCEGQVLSQSNIIPTLEQIKEAFSLFQGKIEQVPPMFSAKK
QQGKKLYELARQGIVVERQPVKISIHTELLSYNYPYLNFRIECSKGTYIRSIAYDLGTKLGCGAHLSNLTRTRSGAFCLE
NCLNGAEIHTVTNLEQNLIRND

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=246, Percent_Identity=33.739837398374, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4503337, Length=199, Percent_Identity=28.1407035175879, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI215599015, Length=199, Percent_Identity=28.1407035175879, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI2367200, Length=205, Percent_Identity=41.4634146341463, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553978, Length=214, Percent_Identity=28.0373831775701, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=131, Percent_Identity=38.9312977099237, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PARUW (Q6MD66)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007758.1
- ProteinModelPortal:   Q6MD66
- SMR:   Q6MD66
- STRING:   Q6MD66
- GeneID:   2780743
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0759
- NMPDR:   fig|264201.1.peg.759
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   Q6MD66
- ProtClustDB:   PRK00989
- BioCyc:   CPRO264201:PC0759-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 29308; Mature: 29308

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: ACT_SITE 77-77

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTCALKNYLEKSQKKESPAKETIVTSLNRSLSTPPSHLEGILLINKPKGKTSFSLVRD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCCHHHHHHHH
LRKRLGVKKIGHAGTLDPFATGVMVMLVGRNYTRLSDQFLLSDKEYIAEAYLGVVTDSYD
HHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
CEGQVLSQSNIIPTLEQIKEAFSLFQGKIEQVPPMFSAKKQQGKKLYELARQGIVVERQP
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCC
VKISIHTELLSYNYPYLNFRIECSKGTYIRSIAYDLGTKLGCGAHLSNLTRTRSGAFCLE
EEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHH
NCLNGAEIHTVTNLEQNLIRND
HCCCCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure
MINTCALKNYLEKSQKKESPAKETIVTSLNRSLSTPPSHLEGILLINKPKGKTSFSLVRD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCEEEEEECCCCCHHHHHHHH
LRKRLGVKKIGHAGTLDPFATGVMVMLVGRNYTRLSDQFLLSDKEYIAEAYLGVVTDSYD
HHHHCCHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
CEGQVLSQSNIIPTLEQIKEAFSLFQGKIEQVPPMFSAKKQQGKKLYELARQGIVVERQP
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCC
VKISIHTELLSYNYPYLNFRIECSKGTYIRSIAYDLGTKLGCGAHLSNLTRTRSGAFCLE
EEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHH
NCLNGAEIHTVTNLEQNLIRND
HCCCCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA